Publicação
Slaughterhouses fungal burden assessment: a contribution for the pursuit of a better assessment strategy
| Resumo: | In slaughterhouses, the biological risk is present not only from the direct or indirect contact with animal matter, but also from the exposure to bioaerosols. Fungal contamination was already reported from the floors and walls of slaughterhouses. This study intends to assess fungal contamination by cultural and molecular methods in poultry, swine/bovine and large animal slaughterhouses. Air samples were collected through an impaction method, while surface samples were collected by the swabbing method and subjected to further macro- and micro-scopic observations. In addition, we collected air samples using the impinger method in order to perform real-time quantitative PCR (qPCR) amplification of genes from specific fungal species, namely A. flavus, A. fumigatus and A. ochraceus complexes. Poultry and swine/bovine slaughterhouses presented each two sampling sites that surpass the guideline of 150 CFU/m3. Scopulariopsis candida was the most frequently isolated (59.5%) in poultry slaughterhouse air; Cladosporium sp. (45.7%) in the swine/bovine slaughterhouse; and Penicillium sp. (80.8%) in the large animal slaughterhouse. Molecular tools successfully amplified DNA from the A. fumigatus complex in six sampling sites where the presence of this fungal species was not identified by conventional methods. This study besides suggesting the indicators that are representative of harmful fungal contamination, also indicates a strategy as a protocol to ensure a proper characterization of fungal occupational exposure. |
|---|---|
| Autores principais: | Viegas, Carla |
| Outros Autores: | Faria, Tiago; dos Santos, Mateus; Carolino, Elisabete; Sabino, Raquel; Gomes, Anita Q.; Viegas, Susana |
| Assunto: | Fungal burden Assessment strategy Slaughterhouses |
| Ano: | 2016 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1865920806541852672 |
|---|---|
| author | Viegas, Carla |
| author2 | Faria, Tiago dos Santos, Mateus Carolino, Elisabete Sabino, Raquel Gomes, Anita Q. Viegas, Susana |
| author2_role | author author author author author author |
| author_facet | Viegas, Carla Viegas, Carla Faria, Tiago dos Santos, Mateus Carolino, Elisabete Sabino, Raquel Gomes, Anita Q. Viegas, Susana Faria, Tiago dos Santos, Mateus Carolino, Elisabete Sabino, Raquel Gomes, Anita Q. Viegas, Susana |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Viegas, Carla\"},{\"Person.name\":\"Faria, Tiago\"},{\"Person.name\":\"dos Santos, Mateus\"},{\"Person.name\":\"Carolino, Elisabete\"},{\"Person.name\":\"Sabino, Raquel\"},{\"Person.name\":\"Gomes, Anita Q.\",\"Person.identifier.orcid\":\"0000-0002-3348-0448\"},{\"Person.name\":\"Viegas, Susana\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Viegas, Carla Faria, Tiago dos Santos, Mateus Carolino, Elisabete Sabino, Raquel Gomes, Anita Q. Viegas, Susana |
| datacite.date.Accepted.fl_str_mv | 2016-03-08T00:00:00Z |
| datacite.date.available.fl_str_mv | 2021-06-25T11:57:42Z |
| datacite.date.embargoed.fl_str_mv | 2021-06-25T11:57:42Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Fungal burden Assessment strategy Slaughterhouses |
| datacite.titles.title.fl_str_mv | Slaughterhouses fungal burden assessment: a contribution for the pursuit of a better assessment strategy |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Viegas, Carla Faria, Tiago dos Santos, Mateus Carolino, Elisabete Sabino, Raquel Gomes, Anita Q. Viegas, Susana |
| dc.date.Accepted.fl_str_mv | 2016-03-08T00:00:00Z |
| dc.date.available.fl_str_mv | 2021-06-25T11:57:42Z |
| dc.date.embargoed.fl_str_mv | 2021-06-25T11:57:42Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10451/48750 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Fungal burden Assessment strategy Slaughterhouses |
| dc.title.fl_str_mv | Slaughterhouses fungal burden assessment: a contribution for the pursuit of a better assessment strategy |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | In slaughterhouses, the biological risk is present not only from the direct or indirect contact with animal matter, but also from the exposure to bioaerosols. Fungal contamination was already reported from the floors and walls of slaughterhouses. This study intends to assess fungal contamination by cultural and molecular methods in poultry, swine/bovine and large animal slaughterhouses. Air samples were collected through an impaction method, while surface samples were collected by the swabbing method and subjected to further macro- and micro-scopic observations. In addition, we collected air samples using the impinger method in order to perform real-time quantitative PCR (qPCR) amplification of genes from specific fungal species, namely A. flavus, A. fumigatus and A. ochraceus complexes. Poultry and swine/bovine slaughterhouses presented each two sampling sites that surpass the guideline of 150 CFU/m3. Scopulariopsis candida was the most frequently isolated (59.5%) in poultry slaughterhouse air; Cladosporium sp. (45.7%) in the swine/bovine slaughterhouse; and Penicillium sp. (80.8%) in the large animal slaughterhouse. Molecular tools successfully amplified DNA from the A. fumigatus complex in six sampling sites where the presence of this fungal species was not identified by conventional methods. This study besides suggesting the indicators that are representative of harmful fungal contamination, also indicates a strategy as a protocol to ensure a proper characterization of fungal occupational exposure. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/88e4d534-5743-4bc7-ac61-ff8d5b6dbe6a/download |
| id | ul_756c1906fb9640ff275760420bdf2dec |
| identifier.url.fl_str_mv | http://hdl.handle.net/10451/48750 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10451/48750 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Viegas, Carla Faria, Tiago dos Santos, Mateus Carolino, Elisabete Sabino, Raquel Gomes, Anita Q. Gomes, Anita Q. https://www.ciencia-id.pt/4B10-E015-52B7 4B10-E015-52B7 http://orcid.org/0000-0002-3348-0448 0000-0002-3348-0448 Viegas, Susana |
| publishDate | 2016 |
| publisher.none.fl_str_mv | MDPI |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engMDPIpt_PTIn slaughterhouses, the biological risk is present not only from the direct or indirect contact with animal matter, but also from the exposure to bioaerosols. Fungal contamination was already reported from the floors and walls of slaughterhouses. This study intends to assess fungal contamination by cultural and molecular methods in poultry, swine/bovine and large animal slaughterhouses. Air samples were collected through an impaction method, while surface samples were collected by the swabbing method and subjected to further macro- and micro-scopic observations. In addition, we collected air samples using the impinger method in order to perform real-time quantitative PCR (qPCR) amplification of genes from specific fungal species, namely A. flavus, A. fumigatus and A. ochraceus complexes. Poultry and swine/bovine slaughterhouses presented each two sampling sites that surpass the guideline of 150 CFU/m3. Scopulariopsis candida was the most frequently isolated (59.5%) in poultry slaughterhouse air; Cladosporium sp. (45.7%) in the swine/bovine slaughterhouse; and Penicillium sp. (80.8%) in the large animal slaughterhouse. Molecular tools successfully amplified DNA from the A. fumigatus complex in six sampling sites where the presence of this fungal species was not identified by conventional methods. This study besides suggesting the indicators that are representative of harmful fungal contamination, also indicates a strategy as a protocol to ensure a proper characterization of fungal occupational exposure.application/pdfpt_PTSlaughterhouses fungal burden assessment: a contribution for the pursuit of a better assessment strategyViegas, CarlaFaria, Tiagodos Santos, MateusCarolino, ElisabeteSabino, RaquelPersonalGomes, Anita Q.DSpacehttp://dspace.org/items/329776ed-aea9-4b80-810c-50e80fc675f9DSpacehttp://dspace.org/items/329776ed-aea9-4b80-810c-50e80fc675f9GomesAnitaCiência IDhttps://www.ciencia-id.pt4B10-E015-52B7ORCIDhttp://orcid.org0000-0002-3348-0448Researcher IDhttps://www.researcherid.comC-3580-2014Scopus Author IDhttps://www.scopus.com7202386033Viegas, SusanaHostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptISSNIsPartOf1661-7827DOIIsPartOf10.3390/ijerph130302972021-06-25T11:57:42Z2016-03-082016-03-08T00:00:00ZHandlehttp://hdl.handle.net/10451/48750http://purl.org/coar/access_right/c_abf2open accessFungal burdenAssessment strategySlaughterhouses653454 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2016-03-08http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/88e4d534-5743-4bc7-ac61-ff8d5b6dbe6a/downloadInternational Journal of Environmental Research and Public Health133 |
| spellingShingle | Slaughterhouses fungal burden assessment: a contribution for the pursuit of a better assessment strategy Slaughterhouses fungal burden assessment: a contribution for the pursuit of a better assessment strategy Viegas, Carla Fungal burden Assessment strategy Slaughterhouses Viegas, Carla Fungal burden Assessment strategy Slaughterhouses |
| status | SINGLETON |
| subject.fl_str_mv | Fungal burden Assessment strategy Slaughterhouses |
| title | Slaughterhouses fungal burden assessment: a contribution for the pursuit of a better assessment strategy |
| title_full | Slaughterhouses fungal burden assessment: a contribution for the pursuit of a better assessment strategy |
| title_fullStr | Slaughterhouses fungal burden assessment: a contribution for the pursuit of a better assessment strategy Slaughterhouses fungal burden assessment: a contribution for the pursuit of a better assessment strategy |
| title_full_unstemmed | Slaughterhouses fungal burden assessment: a contribution for the pursuit of a better assessment strategy Slaughterhouses fungal burden assessment: a contribution for the pursuit of a better assessment strategy |
| title_short | Slaughterhouses fungal burden assessment: a contribution for the pursuit of a better assessment strategy |
| title_sort | Slaughterhouses fungal burden assessment: a contribution for the pursuit of a better assessment strategy |
| topic | Fungal burden Assessment strategy Slaughterhouses |
| topic_facet | Fungal burden Assessment strategy Slaughterhouses |
| url | http://hdl.handle.net/10451/48750 |
| visible | 1 |