Publicação
Antimicrobial effects of essential oils on oral microbiota biofilms: the toothbrush In vitro model
| Resumo: | The present investigation intended to evaluate the bacteriostatic and bactericidal abilities of clove, oregano and thyme essential oils against oral bacteria in planktonic and biofilm states. Furthermore, aiming to mimic everyday conditions, a toothbrush in vitro model was developed. Determination of the minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration and minimum biofilm eradication concentration were achieved using the microdilution procedure. To simulate the toothbrush environment, nylon fibers were inoculated with oral bacteria, which, after incubation to allow biofilm development, were submitted to contact with the essential oils under study. Thyme and oregano essential oils revealed promising antimicrobial effects, both in growth inhibition and the destruction of cells in planktonic and biofilm states, while clove essential oil showed a weaker potential. Regarding the toothbrush in vitro model, observation of the nylon fibers under a magnifying glass proved the essential oil anti-biofilm properties. Considering the effects observed using the in vitro toothbrush model, a realistic approximation to oral biofilm establishment in an everyday use object, a putative application of essential oils as toothbrush sanitizers to help prevent the establishment of bacterial biofilm can be verified. |
|---|---|
| Autores principais: | Aires, Andreia |
| Outros Autores: | BARRETO, ANTONIO; Semedo-Lemsaddek, Teresa |
| Assunto: | Oral microbiota Biofilm Essential oils Antimicrobial activity Toothbrush model |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1865920807012663296 |
|---|---|
| author | Aires, Andreia |
| author2 | BARRETO, ANTONIO Semedo-Lemsaddek, Teresa |
| author2_role | author author |
| author_facet | Aires, Andreia Aires, Andreia BARRETO, ANTONIO Semedo-Lemsaddek, Teresa BARRETO, ANTONIO Semedo-Lemsaddek, Teresa |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Aires, Andreia\"},{\"Person.name\":\"BARRETO, ANTONIO\",\"Person.identifier.orcid\":\"0000-0002-1679-4461\"},{\"Person.name\":\"Semedo-Lemsaddek, Teresa\",\"Person.identifier.orcid\":\"0000-0002-8349-3802\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Aires, Andreia BARRETO, ANTONIO Semedo-Lemsaddek, Teresa |
| datacite.date.Accepted.fl_str_mv | 2021-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2022-01-07T15:42:19Z |
| datacite.date.embargoed.fl_str_mv | 2022-01-07T15:42:19Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Oral microbiota Biofilm Essential oils Antimicrobial activity Toothbrush model |
| datacite.titles.title.fl_str_mv | Antimicrobial effects of essential oils on oral microbiota biofilms: the toothbrush In vitro model |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Aires, Andreia BARRETO, ANTONIO Semedo-Lemsaddek, Teresa |
| dc.date.Accepted.fl_str_mv | 2021-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2022-01-07T15:42:19Z |
| dc.date.embargoed.fl_str_mv | 2022-01-07T15:42:19Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.5/22944 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Oral microbiota Biofilm Essential oils Antimicrobial activity Toothbrush model |
| dc.title.fl_str_mv | Antimicrobial effects of essential oils on oral microbiota biofilms: the toothbrush In vitro model |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The present investigation intended to evaluate the bacteriostatic and bactericidal abilities of clove, oregano and thyme essential oils against oral bacteria in planktonic and biofilm states. Furthermore, aiming to mimic everyday conditions, a toothbrush in vitro model was developed. Determination of the minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration and minimum biofilm eradication concentration were achieved using the microdilution procedure. To simulate the toothbrush environment, nylon fibers were inoculated with oral bacteria, which, after incubation to allow biofilm development, were submitted to contact with the essential oils under study. Thyme and oregano essential oils revealed promising antimicrobial effects, both in growth inhibition and the destruction of cells in planktonic and biofilm states, while clove essential oil showed a weaker potential. Regarding the toothbrush in vitro model, observation of the nylon fibers under a magnifying glass proved the essential oil anti-biofilm properties. Considering the effects observed using the in vitro toothbrush model, a realistic approximation to oral biofilm establishment in an everyday use object, a putative application of essential oils as toothbrush sanitizers to help prevent the establishment of bacterial biofilm can be verified. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/d6ec89e3-c2b2-413c-b4c3-d86c6a972875/download |
| funding.funder.alternateName_str_mv | FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 6817 - DCRRNI ID |
| id | ul_770258be41f862fdd1b9ae78e3d2bd4e |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.5/22944 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10400.5/22944 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Aires, Andreia BARRETO, ANTONIO BARRETO, ANTONIO https://www.ciencia-id.pt/F713-6444-D446 F713-6444-D446 http://orcid.org/0000-0002-1679-4461 0000-0002-1679-4461 Semedo-Lemsaddek, Teresa Semedo-Lemsaddek, Teresa https://www.ciencia-id.pt/A819-03CE-B03E A819-03CE-B03E http://orcid.org/0000-0002-8349-3802 0000-0002-8349-3802 |
| publishDate | 2021 |
| publisher.none.fl_str_mv | MDPI |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engMDPIpt_PTThe present investigation intended to evaluate the bacteriostatic and bactericidal abilities of clove, oregano and thyme essential oils against oral bacteria in planktonic and biofilm states. Furthermore, aiming to mimic everyday conditions, a toothbrush in vitro model was developed. Determination of the minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration and minimum biofilm eradication concentration were achieved using the microdilution procedure. To simulate the toothbrush environment, nylon fibers were inoculated with oral bacteria, which, after incubation to allow biofilm development, were submitted to contact with the essential oils under study. Thyme and oregano essential oils revealed promising antimicrobial effects, both in growth inhibition and the destruction of cells in planktonic and biofilm states, while clove essential oil showed a weaker potential. Regarding the toothbrush in vitro model, observation of the nylon fibers under a magnifying glass proved the essential oil anti-biofilm properties. Considering the effects observed using the in vitro toothbrush model, a realistic approximation to oral biofilm establishment in an everyday use object, a putative application of essential oils as toothbrush sanitizers to help prevent the establishment of bacterial biofilm can be verified.application/pdfpt_PTAntimicrobial effects of essential oils on oral microbiota biofilms: the toothbrush In vitro modelAires, AndreiaPersonalBARRETO, ANTONIODSpacehttp://dspace.org/items/da570f3f-6cbd-496f-8e35-70f1c21c44d2DSpacehttp://dspace.org/items/da570f3f-6cbd-496f-8e35-70f1c21c44d2BARRETOANTONIO SALVADOR FERREIRA HENRIQUES BARRETOCiência IDhttps://www.ciencia-id.ptF713-6444-D446ORCIDhttp://orcid.org0000-0002-1679-4461PersonalSemedo-Lemsaddek, TeresaDSpacehttp://dspace.org/items/a5451375-8abb-4b3b-9e96-e34d334baa52DSpacehttp://dspace.org/items/a5451375-8abb-4b3b-9e96-e34d334baa52Semedo-LemsaddekTeresaCiência IDhttps://www.ciencia-id.ptA819-03CE-B03EORCIDhttp://orcid.org0000-0002-8349-3802Researcher IDhttps://www.researcherid.comJ-3010-2013Scopus Author IDhttps://www.scopus.com25926127100HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptISSNIsPartOf2079-6382DOIIsPartOf10.3390/antibiotics100100212022-01-07T15:42:19Z2021-012021-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.5/22944http://purl.org/coar/access_right/c_abf2open accessOral microbiotaBiofilmEssential oilsAntimicrobial activityToothbrush model1628978 bytesFundação para a Ciência e a TecnologiaCentre for Interdisciplinary Research in Animal Health6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal article2021-01http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/d6ec89e3-c2b2-413c-b4c3-d86c6a972875/downloadAntibiotics10(1):21Basel, Switzerland |
| spellingShingle | Antimicrobial effects of essential oils on oral microbiota biofilms: the toothbrush In vitro model Antimicrobial effects of essential oils on oral microbiota biofilms: the toothbrush In vitro model Aires, Andreia Oral microbiota Biofilm Essential oils Antimicrobial activity Toothbrush model Aires, Andreia Oral microbiota Biofilm Essential oils Antimicrobial activity Toothbrush model |
| status | SINGLETON |
| subject.fl_str_mv | Oral microbiota Biofilm Essential oils Antimicrobial activity Toothbrush model |
| title | Antimicrobial effects of essential oils on oral microbiota biofilms: the toothbrush In vitro model |
| title_full | Antimicrobial effects of essential oils on oral microbiota biofilms: the toothbrush In vitro model |
| title_fullStr | Antimicrobial effects of essential oils on oral microbiota biofilms: the toothbrush In vitro model Antimicrobial effects of essential oils on oral microbiota biofilms: the toothbrush In vitro model |
| title_full_unstemmed | Antimicrobial effects of essential oils on oral microbiota biofilms: the toothbrush In vitro model Antimicrobial effects of essential oils on oral microbiota biofilms: the toothbrush In vitro model |
| title_short | Antimicrobial effects of essential oils on oral microbiota biofilms: the toothbrush In vitro model |
| title_sort | Antimicrobial effects of essential oils on oral microbiota biofilms: the toothbrush In vitro model |
| topic | Oral microbiota Biofilm Essential oils Antimicrobial activity Toothbrush model |
| topic_facet | Oral microbiota Biofilm Essential oils Antimicrobial activity Toothbrush model |
| url | http://hdl.handle.net/10400.5/22944 |
| visible | 1 |