Publicação
Transcriptomic Analyses Reveal That Coffea arabica and Coffea canephora Have More Complex Responses under Combined Heat and Drought than under Individual Stressors
| Resumo: | Increasing exposure to unfavorable temperatures and water deficit imposes major con- straints on most crops worldwide. Despite several studies regarding coffee responses to abiotic stresses, transcriptome modulation due to simultaneous stresses remains poorly understood. This study unravels transcriptomic responses under the combined action of drought and temperature in leaves from the two most traded species: Coffea canephora cv. Conilon Clone 153 (CL153) and C. arabica cv. Icatu. Substantial transcriptomic changes were found, especially in response to the combination of stresses that cannot be explained by an additive effect. A large number of genes were involved in stress responses, with photosynthesis and other physiologically related genes usually being nega- tively affected. In both genotypes, genes encoding for protective proteins, such as dehydrins and heat shock proteins, were positively regulated. Transcription factors (TFs), including MADS-box genes, were down-regulated, although responses were genotype-dependent. In contrast to Icatu, only a few drought- and heat-responsive DEGs were recorded in CL153, which also reacted more significantly in terms of the number of DEGs and enriched GO terms, suggesting a high ability to cope with stresses. This research provides novel insights into the molecular mechanisms underlying leaf Coffea responses to drought and heat, revealing their influence on gene expression. |
|---|---|
| Autores principais: | Marques, Isabel |
| Outros Autores: | Fernandes, Isabel; Paulo, Octávio S.; Rodrigues, Ana P.; Partelli, Fábio L.; DaMatta, Fábio M.; Ribeiro-Barros, Ana I.; Ramalho, José C.; Batista, Dora; Lidon, Fernando C. |
| Assunto: | climate changes coffee drought functional analysis heat stress responses |
| Ano: | 2024 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1866811066012401664 |
|---|---|
| author | Marques, Isabel |
| author2 | Fernandes, Isabel Paulo, Octávio S. Rodrigues, Ana P. Partelli, Fábio L. DaMatta, Fábio M. Ribeiro-Barros, Ana I. Ramalho, José C. Batista, Dora Lidon, Fernando C. |
| author2_role | author author author author author author author author author |
| author_facet | Marques, Isabel Fernandes, Isabel Paulo, Octávio S. Rodrigues, Ana P. Partelli, Fábio L. DaMatta, Fábio M. Ribeiro-Barros, Ana I. Ramalho, José C. Batista, Dora Lidon, Fernando C. |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Marques, Isabel\",\"Person.identifier.orcid\":\"0000-0001-9788-4831\"},{\"Person.name\":\"Fernandes, Isabel\"},{\"Person.name\":\"Paulo, Octávio S.\"},{\"Person.name\":\"Rodrigues, Ana P.\",\"Person.identifier.orcid\":\"0000-0002-9704-6579\"},{\"Person.name\":\"Partelli, Fábio L.\"},{\"Person.name\":\"DaMatta, Fábio M.\"},{\"Person.name\":\"Ribeiro-Barros, Ana I.\",\"Person.identifier.orcid\":\"0000-0002-6071-6460\"},{\"Person.name\":\"Ramalho, José C.\",\"Person.identifier.orcid\":\"0000-0002-7639-7214\"},{\"Person.name\":\"Batista, Dora\",\"Person.identifier.orcid\":\"0000-0003-4031-8999\"},{\"Person.name\":\"Lidon, Fernando C.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Marques, Isabel Fernandes, Isabel Paulo, Octávio S. Rodrigues, Ana P. Partelli, Fábio L. DaMatta, Fábio M. Ribeiro-Barros, Ana I. Ramalho, José C. Batista, Dora Lidon, Fernando C. |
| datacite.date.Accepted.fl_str_mv | 2024-07-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2025-08-05T12:48:03Z |
| datacite.date.embargoed.fl_str_mv | 2025-08-05T12:48:03Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | climate changes coffee drought functional analysis heat stress responses |
| datacite.titles.title.fl_str_mv | Transcriptomic Analyses Reveal That Coffea arabica and Coffea canephora Have More Complex Responses under Combined Heat and Drought than under Individual Stressors |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Marques, Isabel Fernandes, Isabel Paulo, Octávio S. Rodrigues, Ana P. Partelli, Fábio L. DaMatta, Fábio M. Ribeiro-Barros, Ana I. Ramalho, José C. Batista, Dora Lidon, Fernando C. |
| dc.date.Accepted.fl_str_mv | 2024-07-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2025-08-05T12:48:03Z |
| dc.date.embargoed.fl_str_mv | 2025-08-05T12:48:03Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.5/102664 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | climate changes coffee drought functional analysis heat stress responses |
| dc.title.fl_str_mv | Transcriptomic Analyses Reveal That Coffea arabica and Coffea canephora Have More Complex Responses under Combined Heat and Drought than under Individual Stressors |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Increasing exposure to unfavorable temperatures and water deficit imposes major con- straints on most crops worldwide. Despite several studies regarding coffee responses to abiotic stresses, transcriptome modulation due to simultaneous stresses remains poorly understood. This study unravels transcriptomic responses under the combined action of drought and temperature in leaves from the two most traded species: Coffea canephora cv. Conilon Clone 153 (CL153) and C. arabica cv. Icatu. Substantial transcriptomic changes were found, especially in response to the combination of stresses that cannot be explained by an additive effect. A large number of genes were involved in stress responses, with photosynthesis and other physiologically related genes usually being nega- tively affected. In both genotypes, genes encoding for protective proteins, such as dehydrins and heat shock proteins, were positively regulated. Transcription factors (TFs), including MADS-box genes, were down-regulated, although responses were genotype-dependent. In contrast to Icatu, only a few drought- and heat-responsive DEGs were recorded in CL153, which also reacted more significantly in terms of the number of DEGs and enriched GO terms, suggesting a high ability to cope with stresses. This research provides novel insights into the molecular mechanisms underlying leaf Coffea responses to drought and heat, revealing their influence on gene expression. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/f9bc749a-fd9d-4f3d-9db8-8102ad9f1cf2/download |
| funding.funder.alternateName_str_mv | FCT FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | CEEC IND4ed 6817 - DCRRNI ID 6817 - DCRRNI ID |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10400.5/102664 |
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| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10400.5/102664 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Marques, Isabel Marques, Isabel https://www.ciencia-id.pt/A71A-611E-44B3 A71A-611E-44B3 http://orcid.org/0000-0001-9788-4831 0000-0001-9788-4831 Fernandes, Isabel Paulo, Octávio S. Rodrigues, Ana P. Rodrigues, Ana P. https://www.ciencia-id.pt/0313-F387-CFFE 0313-F387-CFFE http://orcid.org/0000-0002-9704-6579 0000-0002-9704-6579 Partelli, Fábio L. DaMatta, Fábio M. Ribeiro-Barros, Ana I. Ribeiro-Barros, Ana I. https://www.ciencia-id.pt/081F-E3CE-9D52 081F-E3CE-9D52 http://orcid.org/0000-0002-6071-6460 0000-0002-6071-6460 Ramalho, José C. Ramalho, José C. https://www.ciencia-id.pt/2C14-07F8-4EFE 2C14-07F8-4EFE http://orcid.org/0000-0002-7639-7214 0000-0002-7639-7214 Batista, Dora Batista, Dora https://www.ciencia-id.pt/621A-E288-BDF5 621A-E288-BDF5 http://orcid.org/0000-0003-4031-8999 0000-0003-4031-8999 Lidon, Fernando C. |
| publishDate | 2024 |
| publisher.none.fl_str_mv | MDPI |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engMDPIpt_PTIncreasing exposure to unfavorable temperatures and water deficit imposes major con- straints on most crops worldwide. Despite several studies regarding coffee responses to abiotic stresses, transcriptome modulation due to simultaneous stresses remains poorly understood. This study unravels transcriptomic responses under the combined action of drought and temperature in leaves from the two most traded species: Coffea canephora cv. Conilon Clone 153 (CL153) and C. arabica cv. Icatu. Substantial transcriptomic changes were found, especially in response to the combination of stresses that cannot be explained by an additive effect. A large number of genes were involved in stress responses, with photosynthesis and other physiologically related genes usually being nega- tively affected. In both genotypes, genes encoding for protective proteins, such as dehydrins and heat shock proteins, were positively regulated. Transcription factors (TFs), including MADS-box genes, were down-regulated, although responses were genotype-dependent. In contrast to Icatu, only a few drought- and heat-responsive DEGs were recorded in CL153, which also reacted more significantly in terms of the number of DEGs and enriched GO terms, suggesting a high ability to cope with stresses. This research provides novel insights into the molecular mechanisms underlying leaf Coffea responses to drought and heat, revealing their influence on gene expression.application/pdfpt_PTTranscriptomic Analyses Reveal That Coffea arabica and Coffea canephora Have More Complex Responses under Combined Heat and Drought than under Individual StressorsPersonalMarques, IsabelDSpacehttp://dspace.org/items/554ac833-5950-4e34-8e69-cb36c20e8847DSpacehttp://dspace.org/items/554ac833-5950-4e34-8e69-cb36c20e8847Dos Santos Lourenço MarquesIsabel CristinaCiência IDhttps://www.ciencia-id.ptA71A-611E-44B3ORCIDhttp://orcid.org0000-0001-9788-4831Scopus Author IDhttps://www.scopus.com19337527300Fernandes, IsabelPaulo, Octávio S.PersonalRodrigues, Ana P.DSpacehttp://dspace.org/items/1f2e33db-63d7-4640-91ca-2f4bb120d39dDSpacehttp://dspace.org/items/1f2e33db-63d7-4640-91ca-2f4bb120d39dDias RodriguesAna PaulaCiência IDhttps://www.ciencia-id.pt0313-F387-CFFEORCIDhttp://orcid.org0000-0002-9704-6579Scopus Author IDhttps://www.scopus.com9940165000Partelli, Fábio L.DaMatta, Fábio M.PersonalRibeiro-Barros, Ana I.DSpacehttp://dspace.org/items/589221f0-6470-48c0-be2c-a48386fcbf89DSpacehttp://dspace.org/items/589221f0-6470-48c0-be2c-a48386fcbf89RIBEIRO-BARROSANA ICiência IDhttps://www.ciencia-id.pt081F-E3CE-9D52ORCIDhttp://orcid.org0000-0002-6071-6460Researcher IDhttps://www.researcherid.comJ-4322-2017Scopus Author IDhttps://www.scopus.com35557486600PersonalRamalho, José C.DSpacehttp://dspace.org/items/2943728c-2bda-4707-b35d-8395ae36c7edDSpacehttp://dspace.org/items/2943728c-2bda-4707-b35d-8395ae36c7edRamalhoJoséCiência IDhttps://www.ciencia-id.pt2C14-07F8-4EFEORCIDhttp://orcid.org0000-0002-7639-7214Researcher IDhttps://www.researcherid.comG-2427-2014Scopus Author IDhttps://www.scopus.com57196449038PersonalBatista, DoraDSpacehttp://dspace.org/items/9d3f84b6-fa9c-4a39-81a4-d7642c2ba0bfDSpacehttp://dspace.org/items/9d3f84b6-fa9c-4a39-81a4-d7642c2ba0bfBatistaDoraCiência IDhttps://www.ciencia-id.pt621A-E288-BDF5ORCIDhttp://orcid.org0000-0003-4031-8999Scopus Author IDhttps://www.scopus.com6603861581Lidon, Fernando C.HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptDOIIsPartOf10.3390/ijms251479952025-08-05T12:48:03Z2024-072024-07-01T00:00:00ZHandlehttp://hdl.handle.net/10400.5/102664http://purl.org/coar/access_right/c_abf2open accessclimate changescoffeedroughtfunctional analysisheatstress responses2733553 bytesFundação para a Ciência e a TecnologiaSUSTROP: SUStainable management of TROPpical resources: functional genomic innovation into the service of local communitiesCEEC IND4edCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaForest Research Centre6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaLaboratory for Sustainable Land Use and Ecosystem Services6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal article2024-07http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/f9bc749a-fd9d-4f3d-9db8-8102ad9f1cf2/downloadInternational Journal of Molecular Sciences25147995 |
| spellingShingle | Transcriptomic Analyses Reveal That Coffea arabica and Coffea canephora Have More Complex Responses under Combined Heat and Drought than under Individual Stressors Marques, Isabel climate changes coffee drought functional analysis heat stress responses |
| status | SINGLETON |
| subject.fl_str_mv | climate changes coffee drought functional analysis heat stress responses |
| title | Transcriptomic Analyses Reveal That Coffea arabica and Coffea canephora Have More Complex Responses under Combined Heat and Drought than under Individual Stressors |
| title_full | Transcriptomic Analyses Reveal That Coffea arabica and Coffea canephora Have More Complex Responses under Combined Heat and Drought than under Individual Stressors |
| title_fullStr | Transcriptomic Analyses Reveal That Coffea arabica and Coffea canephora Have More Complex Responses under Combined Heat and Drought than under Individual Stressors |
| title_full_unstemmed | Transcriptomic Analyses Reveal That Coffea arabica and Coffea canephora Have More Complex Responses under Combined Heat and Drought than under Individual Stressors |
| title_short | Transcriptomic Analyses Reveal That Coffea arabica and Coffea canephora Have More Complex Responses under Combined Heat and Drought than under Individual Stressors |
| title_sort | Transcriptomic Analyses Reveal That Coffea arabica and Coffea canephora Have More Complex Responses under Combined Heat and Drought than under Individual Stressors |
| topic | climate changes coffee drought functional analysis heat stress responses |
| topic_facet | climate changes coffee drought functional analysis heat stress responses |
| url | http://hdl.handle.net/10400.5/102664 |
| visible | 1 |