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Enantiopure Indolizinoindolones with in vitro activity against blood- and liver-stage malaria parasites

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Resumo:Malaria continues to be a major cause of morbidity and mortality to this day, and resistance to drugs like chloroquine has led to an urgent need to discover novel chemical entities aimed at new targets. Here, we report the discovery of a novel class of potential antimalarial compounds containing an indolizinoindolone scaffold. These novel enantiopure indolizinoindolones were synthesized, in good-to-excellent yields and excellent diastereoselectivities, by cyclocondensation reaction of (S)- or (R)-tryptophanol and 2-acyl benzoic acids, followed by intramolecular α-amidoalkylation. Interestingly, we were able to synthesize for the first time 7,13b-cis indolizinoindolones in a two-step route. The novel compounds showed promising activity against erythrocytic stages of the human malaria parasite, Plasmodium falciparum, and liver stages of the rodent parasite Plasmodium berghei. In particular, an (S)-tryptophanol-derived isoindolinone was identified as a promising starting scaffold to search for novel antimalarials, combining excellent activity against both stages of the parasite's life cycle with low cytotoxicity and excellent metabolic and chemical stability in vitro.
Autores principais:Pereira, Nuno A. L.
Outros Autores:Monteiro, Ângelo; Machado, Marta; Gut, Jiri; Molins, Elies; Perry, Maria Jesus; Dourado, Jorge; Moreira, Rui; Rosenthal, Philip J.; Prudêncio, Miguel; Santos, Maria M. M.
Assunto:Diastereoselectivity Drug design Dual-stage antimalarials Malaria Nitrogen heterocycles
Ano:2015
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso restrito
Instituição associada:Universidade de Lisboa
Idioma:inglês
Origem:Repositório da Universidade de Lisboa
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author Pereira, Nuno A. L.
author2 Monteiro, Ângelo
Machado, Marta
Gut, Jiri
Molins, Elies
Perry, Maria Jesus
Dourado, Jorge
Moreira, Rui
Rosenthal, Philip J.
Prudêncio, Miguel
Santos, Maria M. M.
author2_role author
author
author
author
author
author
author
author
author
author
author_facet Pereira, Nuno A. L.
Monteiro, Ângelo
Machado, Marta
Gut, Jiri
Molins, Elies
Perry, Maria Jesus
Dourado, Jorge
Moreira, Rui
Rosenthal, Philip J.
Prudêncio, Miguel
Santos, Maria M. M.
author_role author
contributor_name_str_mv Repositório Científico de Acesso Aberto da ULisboa
country_str PT
creators_json_txt [{\"Person.name\":\"Pereira, Nuno A. L.\"},{\"Person.name\":\"Monteiro, Ângelo\",\"Person.identifier.orcid\":\"0000-0002-3262-4374\"},{\"Person.name\":\"Machado, Marta\"},{\"Person.name\":\"Gut, Jiri\"},{\"Person.name\":\"Molins, Elies\"},{\"Person.name\":\"Perry, Maria Jesus\",\"Person.identifier.orcid\":\"0000-0002-8110-2740\"},{\"Person.name\":\"Dourado, Jorge\"},{\"Person.name\":\"Moreira, Rui\",\"Person.identifier.orcid\":\"0000-0003-0727-9852\"},{\"Person.name\":\"Rosenthal, Philip J.\"},{\"Person.name\":\"Prudêncio, Miguel\",\"Person.identifier.orcid\":\"0000-0003-1746-6029\"},{\"Person.name\":\"Santos, Maria M. M.\",\"Person.identifier.orcid\":\"0000-0002-2239-9353\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Repositório Científico de Acesso Aberto da ULisboa
datacite.creators.creator.creatorName.fl_str_mv Pereira, Nuno A. L.
Monteiro, Ângelo
Machado, Marta
Gut, Jiri
Molins, Elies
Perry, Maria Jesus
Dourado, Jorge
Moreira, Rui
Rosenthal, Philip J.
Prudêncio, Miguel
Santos, Maria M. M.
datacite.date.Accepted.fl_str_mv 2015-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2022-02-11T16:01:53Z
datacite.date.embargoed.fl_str_mv 2022-02-11T16:01:53Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_16ec
datacite.subjects.subject.fl_str_mv Diastereoselectivity
Drug design
Dual-stage antimalarials
Malaria
Nitrogen heterocycles
datacite.titles.title.fl_str_mv Enantiopure Indolizinoindolones with in vitro activity against blood- and liver-stage malaria parasites
dc.contributor.none.fl_str_mv Repositório Científico de Acesso Aberto da ULisboa
dc.creator.none.fl_str_mv Pereira, Nuno A. L.
Monteiro, Ângelo
Machado, Marta
Gut, Jiri
Molins, Elies
Perry, Maria Jesus
Dourado, Jorge
Moreira, Rui
Rosenthal, Philip J.
Prudêncio, Miguel
Santos, Maria M. M.
dc.date.Accepted.fl_str_mv 2015-01-01T00:00:00Z
dc.date.available.fl_str_mv 2022-02-11T16:01:53Z
dc.date.embargoed.fl_str_mv 2022-02-11T16:01:53Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10451/51232
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv Wiley
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_16ec
dc.subject.none.fl_str_mv Diastereoselectivity
Drug design
Dual-stage antimalarials
Malaria
Nitrogen heterocycles
dc.title.fl_str_mv Enantiopure Indolizinoindolones with in vitro activity against blood- and liver-stage malaria parasites
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description Malaria continues to be a major cause of morbidity and mortality to this day, and resistance to drugs like chloroquine has led to an urgent need to discover novel chemical entities aimed at new targets. Here, we report the discovery of a novel class of potential antimalarial compounds containing an indolizinoindolone scaffold. These novel enantiopure indolizinoindolones were synthesized, in good-to-excellent yields and excellent diastereoselectivities, by cyclocondensation reaction of (S)- or (R)-tryptophanol and 2-acyl benzoic acids, followed by intramolecular α-amidoalkylation. Interestingly, we were able to synthesize for the first time 7,13b-cis indolizinoindolones in a two-step route. The novel compounds showed promising activity against erythrocytic stages of the human malaria parasite, Plasmodium falciparum, and liver stages of the rodent parasite Plasmodium berghei. In particular, an (S)-tryptophanol-derived isoindolinone was identified as a promising starting scaffold to search for novel antimalarials, combining excellent activity against both stages of the parasite's life cycle with low cytotoxicity and excellent metabolic and chemical stability in vitro.
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fulltext.url.fl_str_mv https://repositorio.ulisboa.pt/bitstreams/08e2543d-deec-418a-9588-032990f684a5/download
funding.funder.alternateName_str_mv FCT
FCT
funding.funder.identifier_str_mv http://doi.org/10.13039/501100001871
http://doi.org/10.13039/501100001871
funding.funder.name_str_mv Fundação para a Ciência e a Tecnologia
Fundação para a Ciência e a Tecnologia
funding.name_str_mv 3599-PPCDT
3599-PPCDT
id ul_85d0d2e7e7e232dc6f4dda523de7a4a7
identifier.url.fl_str_mv http://hdl.handle.net/10451/51232
instacron_str ul
institution Universidade de Lisboa
instname_str Universidade de Lisboa
language eng
network_acronym_str ul
network_name_str Repositório da Universidade de Lisboa
oai_identifier_str oai:repositorio.ulisboa.pt:10451/51232
organization_str_mv urn:organizationAcronym:ul
person_str_mv Pereira, Nuno A. L.
Monteiro, Ângelo
Monteiro, Ângelo
http://orcid.org/0000-0002-3262-4374
0000-0002-3262-4374
Machado, Marta
Gut, Jiri
Molins, Elies
Perry, Maria Jesus
Perry, Maria Jesus
https://www.ciencia-id.pt/A516-1A46-D715
A516-1A46-D715
http://orcid.org/0000-0002-8110-2740
0000-0002-8110-2740
Dourado, Jorge
Moreira, Rui
Moreira, Rui
https://www.ciencia-id.pt/8B11-E8C0-DFD0
8B11-E8C0-DFD0
http://orcid.org/0000-0003-0727-9852
0000-0003-0727-9852
Rosenthal, Philip J.
Prudêncio, Miguel
Prudêncio, Miguel
https://www.ciencia-id.pt/5511-16ED-48E0
5511-16ED-48E0
http://orcid.org/0000-0003-1746-6029
0000-0003-1746-6029
Santos, Maria M. M.
Santos, Maria M. M.
https://www.ciencia-id.pt/5910-2BF6-5F65
5910-2BF6-5F65
http://orcid.org/0000-0002-2239-9353
0000-0002-2239-9353
publishDate 2015
publisher.none.fl_str_mv Wiley
reponame_str Repositório da Universidade de Lisboa
repository_id_str urn:repositoryAcronym:ul
service_str_mv urn:repositoryAcronym:ul
spelling engWileypt_PTMalaria continues to be a major cause of morbidity and mortality to this day, and resistance to drugs like chloroquine has led to an urgent need to discover novel chemical entities aimed at new targets. Here, we report the discovery of a novel class of potential antimalarial compounds containing an indolizinoindolone scaffold. These novel enantiopure indolizinoindolones were synthesized, in good-to-excellent yields and excellent diastereoselectivities, by cyclocondensation reaction of (S)- or (R)-tryptophanol and 2-acyl benzoic acids, followed by intramolecular α-amidoalkylation. Interestingly, we were able to synthesize for the first time 7,13b-cis indolizinoindolones in a two-step route. The novel compounds showed promising activity against erythrocytic stages of the human malaria parasite, Plasmodium falciparum, and liver stages of the rodent parasite Plasmodium berghei. In particular, an (S)-tryptophanol-derived isoindolinone was identified as a promising starting scaffold to search for novel antimalarials, combining excellent activity against both stages of the parasite's life cycle with low cytotoxicity and excellent metabolic and chemical stability in vitro.application/pdfpt_PTEnantiopure Indolizinoindolones with in vitro activity against blood- and liver-stage malaria parasitesPereira, Nuno A. L.PersonalMonteiro, ÂngeloDSpacehttp://dspace.org/items/0165a0df-71b9-4b11-89cd-27187b1289a7DSpacehttp://dspace.org/items/0165a0df-71b9-4b11-89cd-27187b1289a7MonteiroÂngeloORCIDhttp://orcid.org0000-0002-3262-4374Machado, MartaGut, JiriMolins, EliesPersonalPerry, Maria JesusDSpacehttp://dspace.org/items/710e88bf-5294-4176-ab7c-784fc8717e90DSpacehttp://dspace.org/items/710e88bf-5294-4176-ab7c-784fc8717e90PerryMaria JesusCiência IDhttps://www.ciencia-id.ptA516-1A46-D715ORCIDhttp://orcid.org0000-0002-8110-2740Researcher IDhttps://www.researcherid.comE-1746-2015Scopus Author IDhttps://www.scopus.com55254669000Dourado, JorgePersonalMoreira, RuiDSpacehttp://dspace.org/items/7a2ba2a9-2433-4055-9264-bc3164c6cd0dDSpacehttp://dspace.org/items/7a2ba2a9-2433-4055-9264-bc3164c6cd0dMoreiraRuiCiência IDhttps://www.ciencia-id.pt8B11-E8C0-DFD0ORCIDhttp://orcid.org0000-0003-0727-9852Scopus Author IDhttps://www.scopus.com7102554088Rosenthal, Philip J.PersonalPrudêncio, MiguelDSpacehttp://dspace.org/items/80e4e74d-bf34-4a71-8530-cc3280543b65DSpacehttp://dspace.org/items/80e4e74d-bf34-4a71-8530-cc3280543b65PrudêncioMiguelCiência IDhttps://www.ciencia-id.pt5511-16ED-48E0ORCIDhttp://orcid.org0000-0003-1746-6029Scopus Author IDhttps://www.scopus.com6603561872PersonalSantos, Maria M. M.DSpacehttp://dspace.org/items/07fcf050-9273-4a16-9053-47d46229729aDSpacehttp://dspace.org/items/07fcf050-9273-4a16-9053-47d46229729aSantosMariaCiência IDhttps://www.ciencia-id.pt5910-2BF6-5F65ORCIDhttp://orcid.org0000-0002-2239-9353Researcher IDhttps://www.researcherid.comI-7189-2013Scopus Author IDhttps://www.scopus.com8634544500Scopus Author IDhttps://www.scopus.com57200135931HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptISSNIsPartOf1860-7179DOIIsPartOf10.1002/cmdc.2015004292022-02-11T16:01:53Z20152015-01-01T00:00:00ZHandlehttp://hdl.handle.net/10451/51232http://purl.org/coar/access_right/c_16ecrestricted accessDiastereoselectivityDrug designDual-stage antimalarialsMalariaNitrogen heterocycles1703642 bytesFundação para a Ciência e a TecnologiaEnantioselective synthesis of indole alkaloids and their application as NMDA antagonists3599-PPCDTCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaUtilization of host cell resources by the malaria parasite in the liver3599-PPCDTCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/08e2543d-deec-418a-9588-032990f684a5/downloadChemMedChem101220802089
spellingShingle Enantiopure Indolizinoindolones with in vitro activity against blood- and liver-stage malaria parasites
Pereira, Nuno A. L.
Diastereoselectivity
Drug design
Dual-stage antimalarials
Malaria
Nitrogen heterocycles
status SINGLETON
subject.fl_str_mv Diastereoselectivity
Drug design
Dual-stage antimalarials
Malaria
Nitrogen heterocycles
title Enantiopure Indolizinoindolones with in vitro activity against blood- and liver-stage malaria parasites
title_full Enantiopure Indolizinoindolones with in vitro activity against blood- and liver-stage malaria parasites
title_fullStr Enantiopure Indolizinoindolones with in vitro activity against blood- and liver-stage malaria parasites
title_full_unstemmed Enantiopure Indolizinoindolones with in vitro activity against blood- and liver-stage malaria parasites
title_short Enantiopure Indolizinoindolones with in vitro activity against blood- and liver-stage malaria parasites
title_sort Enantiopure Indolizinoindolones with in vitro activity against blood- and liver-stage malaria parasites
topic Diastereoselectivity
Drug design
Dual-stage antimalarials
Malaria
Nitrogen heterocycles
topic_facet Diastereoselectivity
Drug design
Dual-stage antimalarials
Malaria
Nitrogen heterocycles
url http://hdl.handle.net/10451/51232
visible 1