Publicação
The retinoid X receptor: a nuclear receptor that modulates the sleep-wake cycle in rats
| Resumo: | Rationale: The nuclear receptor retinoid X receptor (RXR) belongs to a nuclear receptor superfamily that modulates diverse functions via homodimerization with itself or several other nuclear receptors, including PPARα. While the activation of PPARα by natural or synthetic agonists regulates the sleep-wake cycle, the role of RXR in the sleep modulation is unknown. Objectives: We investigated the effects of bexarotene (Bexa, a RXR agonist) or UVI 3003 (UVI, a RXR antagonist) on sleep, sleep homeostasis, levels of neurochemical related to sleep modulation, and c-Fos and NeuN expression. Methods: The sleep-wake cycle and sleep homeostasis were analyzed after application of Bexa or UVI. Moreover, we also evaluated whether Bexa or UVI could induce effects on dopamine, serotonin, norepinephrine epinephrine, adenosine, and acetylcholine contents, collected from either the nucleus accumbens or basal forebrain. In addition, c-Fos and NeuN expression in the hypothalamus was determined after Bexa or UVI treatments. Results: Systemic application of Bexa (1 mM, i.p.) attenuated slow-wave sleep and rapid eye movement sleep. In addition, Bexa increased the levels of dopamine, serotonin, norepinephrine epinephrine, adenosine, and acetylcholine sampled from either the nucleus accumbens or basal forebrain. Moreover, Bexa blocked the sleep rebound period after total sleep deprivation, increased in the hypothalamus the expression of c-Fos, and decreased NeuN activity. Remarkably, UVI 3003 (1 mM, i.p.) induced opposite effects in sleep, sleep homeostasis, neurochemicals levels, and c-Fos and NeuN activity. Conclusions: The administration of RXR agonist or antagonist significantly impaired the sleep-wake cycle and exerted effects on the levels of neurochemicals related to sleep modulation. Moreover, Bexa or UVI administration significantly affected c-Fos and NeuN expression in the hypothalamus. Our findings highlight the neurobiological role of RXR on sleep modulation. |
|---|---|
| Autores principais: | Murillo-Rodríguez, Eric |
| Outros Autores: | Millán-Aldaco, Diana; Arankowsky-Sandoval, Gloria; Yamamoto, Tetsuya; Cid, Luis; Monteiro, Diogo; Rocha, Nuno Barbosa; Telles-Correia, Diogo; Teixeira, Diogo S.; Veras, André Barciela; Budde, Henning; Machado, Sérgio; Imperatori, Claudio; Torterolo, Pablo |
| Assunto: | Adenosine Dopamine Retinoid X receptor Serotonin Sleep |
| Ano: | 2020 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1866809809353834496 |
|---|---|
| author | Murillo-Rodríguez, Eric |
| author2 | Millán-Aldaco, Diana Arankowsky-Sandoval, Gloria Yamamoto, Tetsuya Cid, Luis Monteiro, Diogo Rocha, Nuno Barbosa Telles-Correia, Diogo Teixeira, Diogo S. Veras, André Barciela Budde, Henning Machado, Sérgio Imperatori, Claudio Torterolo, Pablo |
| author2_role | author author author author author author author author author author author author author |
| author_facet | Murillo-Rodríguez, Eric Millán-Aldaco, Diana Arankowsky-Sandoval, Gloria Yamamoto, Tetsuya Cid, Luis Monteiro, Diogo Rocha, Nuno Barbosa Telles-Correia, Diogo Teixeira, Diogo S. Veras, André Barciela Budde, Henning Machado, Sérgio Imperatori, Claudio Torterolo, Pablo |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Murillo-Rodríguez, Eric\"},{\"Person.name\":\"Millán-Aldaco, Diana\"},{\"Person.name\":\"Arankowsky-Sandoval, Gloria\"},{\"Person.name\":\"Yamamoto, Tetsuya\"},{\"Person.name\":\"Cid, Luis\"},{\"Person.name\":\"Monteiro, Diogo\"},{\"Person.name\":\"Rocha, Nuno Barbosa\"},{\"Person.name\":\"Telles-Correia, Diogo\",\"Person.identifier.orcid\":\"0000-0003-4217-2823\"},{\"Person.name\":\"Teixeira, Diogo S.\"},{\"Person.name\":\"Veras, André Barciela\"},{\"Person.name\":\"Budde, Henning\"},{\"Person.name\":\"Machado, Sérgio\"},{\"Person.name\":\"Imperatori, Claudio\"},{\"Person.name\":\"Torterolo, Pablo\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Murillo-Rodríguez, Eric Millán-Aldaco, Diana Arankowsky-Sandoval, Gloria Yamamoto, Tetsuya Cid, Luis Monteiro, Diogo Rocha, Nuno Barbosa Telles-Correia, Diogo Teixeira, Diogo S. Veras, André Barciela Budde, Henning Machado, Sérgio Imperatori, Claudio Torterolo, Pablo |
| datacite.date.Accepted.fl_str_mv | 2020-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2022-06-06T15:21:39Z |
| datacite.date.embargoed.fl_str_mv | 2022-06-06T15:21:39Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Adenosine Dopamine Retinoid X receptor Serotonin Sleep |
| datacite.titles.title.fl_str_mv | The retinoid X receptor: a nuclear receptor that modulates the sleep-wake cycle in rats |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Murillo-Rodríguez, Eric Millán-Aldaco, Diana Arankowsky-Sandoval, Gloria Yamamoto, Tetsuya Cid, Luis Monteiro, Diogo Rocha, Nuno Barbosa Telles-Correia, Diogo Teixeira, Diogo S. Veras, André Barciela Budde, Henning Machado, Sérgio Imperatori, Claudio Torterolo, Pablo |
| dc.date.Accepted.fl_str_mv | 2020-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2022-06-06T15:21:39Z |
| dc.date.embargoed.fl_str_mv | 2022-06-06T15:21:39Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10451/53293 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Adenosine Dopamine Retinoid X receptor Serotonin Sleep |
| dc.title.fl_str_mv | The retinoid X receptor: a nuclear receptor that modulates the sleep-wake cycle in rats |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Rationale: The nuclear receptor retinoid X receptor (RXR) belongs to a nuclear receptor superfamily that modulates diverse functions via homodimerization with itself or several other nuclear receptors, including PPARα. While the activation of PPARα by natural or synthetic agonists regulates the sleep-wake cycle, the role of RXR in the sleep modulation is unknown. Objectives: We investigated the effects of bexarotene (Bexa, a RXR agonist) or UVI 3003 (UVI, a RXR antagonist) on sleep, sleep homeostasis, levels of neurochemical related to sleep modulation, and c-Fos and NeuN expression. Methods: The sleep-wake cycle and sleep homeostasis were analyzed after application of Bexa or UVI. Moreover, we also evaluated whether Bexa or UVI could induce effects on dopamine, serotonin, norepinephrine epinephrine, adenosine, and acetylcholine contents, collected from either the nucleus accumbens or basal forebrain. In addition, c-Fos and NeuN expression in the hypothalamus was determined after Bexa or UVI treatments. Results: Systemic application of Bexa (1 mM, i.p.) attenuated slow-wave sleep and rapid eye movement sleep. In addition, Bexa increased the levels of dopamine, serotonin, norepinephrine epinephrine, adenosine, and acetylcholine sampled from either the nucleus accumbens or basal forebrain. Moreover, Bexa blocked the sleep rebound period after total sleep deprivation, increased in the hypothalamus the expression of c-Fos, and decreased NeuN activity. Remarkably, UVI 3003 (1 mM, i.p.) induced opposite effects in sleep, sleep homeostasis, neurochemicals levels, and c-Fos and NeuN activity. Conclusions: The administration of RXR agonist or antagonist significantly impaired the sleep-wake cycle and exerted effects on the levels of neurochemicals related to sleep modulation. Moreover, Bexa or UVI administration significantly affected c-Fos and NeuN expression in the hypothalamus. Our findings highlight the neurobiological role of RXR on sleep modulation. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/a9bddbf4-1d46-4624-be46-04c15c620763/download |
| id | ul_a00a90ed06dc65b8e00c6c21e8de9d77 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10451/53293 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10451/53293 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Murillo-Rodríguez, Eric Millán-Aldaco, Diana Arankowsky-Sandoval, Gloria Yamamoto, Tetsuya Cid, Luis Monteiro, Diogo Rocha, Nuno Barbosa Telles-Correia, Diogo Telles-Correia, Diogo https://www.ciencia-id.pt/B219-3860-D2C5 B219-3860-D2C5 http://orcid.org/0000-0003-4217-2823 0000-0003-4217-2823 Teixeira, Diogo S. Veras, André Barciela Budde, Henning Machado, Sérgio Imperatori, Claudio Torterolo, Pablo |
| publishDate | 2020 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engElsevierpt_PTRationale: The nuclear receptor retinoid X receptor (RXR) belongs to a nuclear receptor superfamily that modulates diverse functions via homodimerization with itself or several other nuclear receptors, including PPARα. While the activation of PPARα by natural or synthetic agonists regulates the sleep-wake cycle, the role of RXR in the sleep modulation is unknown. Objectives: We investigated the effects of bexarotene (Bexa, a RXR agonist) or UVI 3003 (UVI, a RXR antagonist) on sleep, sleep homeostasis, levels of neurochemical related to sleep modulation, and c-Fos and NeuN expression. Methods: The sleep-wake cycle and sleep homeostasis were analyzed after application of Bexa or UVI. Moreover, we also evaluated whether Bexa or UVI could induce effects on dopamine, serotonin, norepinephrine epinephrine, adenosine, and acetylcholine contents, collected from either the nucleus accumbens or basal forebrain. In addition, c-Fos and NeuN expression in the hypothalamus was determined after Bexa or UVI treatments. Results: Systemic application of Bexa (1 mM, i.p.) attenuated slow-wave sleep and rapid eye movement sleep. In addition, Bexa increased the levels of dopamine, serotonin, norepinephrine epinephrine, adenosine, and acetylcholine sampled from either the nucleus accumbens or basal forebrain. Moreover, Bexa blocked the sleep rebound period after total sleep deprivation, increased in the hypothalamus the expression of c-Fos, and decreased NeuN activity. Remarkably, UVI 3003 (1 mM, i.p.) induced opposite effects in sleep, sleep homeostasis, neurochemicals levels, and c-Fos and NeuN activity. Conclusions: The administration of RXR agonist or antagonist significantly impaired the sleep-wake cycle and exerted effects on the levels of neurochemicals related to sleep modulation. Moreover, Bexa or UVI administration significantly affected c-Fos and NeuN expression in the hypothalamus. Our findings highlight the neurobiological role of RXR on sleep modulation.application/pdfpt_PTThe retinoid X receptor: a nuclear receptor that modulates the sleep-wake cycle in ratsMurillo-Rodríguez, EricMillán-Aldaco, DianaArankowsky-Sandoval, GloriaYamamoto, TetsuyaCid, LuisMonteiro, DiogoRocha, Nuno BarbosaPersonalTelles-Correia, DiogoDSpacehttp://dspace.org/items/7eec6382-2f4a-45b0-b4be-7b6ac9a77786DSpacehttp://dspace.org/items/7eec6382-2f4a-45b0-b4be-7b6ac9a77786Telles-CorreiaDiogoCiência IDhttps://www.ciencia-id.ptB219-3860-D2C5ORCIDhttp://orcid.org0000-0003-4217-2823Scopus Author IDhttps://www.scopus.com15030253900Teixeira, Diogo S.Veras, André BarcielaBudde, HenningMachado, SérgioImperatori, ClaudioTorterolo, PabloHostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptISSNIsPartOf0033-3158DOIIsPartOf10.1007/s00213-020-05518-82022-06-06T15:21:39Z20202020-01-01T00:00:00ZHandlehttp://hdl.handle.net/10451/53293http://purl.org/coar/access_right/c_16ecrestricted accessAdenosineDopamineRetinoid X receptorSerotoninSleep1865849 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/a9bddbf4-1d46-4624-be46-04c15c620763/downloadPsychopharmacology237720552073 |
| spellingShingle | The retinoid X receptor: a nuclear receptor that modulates the sleep-wake cycle in rats Murillo-Rodríguez, Eric Adenosine Dopamine Retinoid X receptor Serotonin Sleep |
| status | SINGLETON |
| subject.fl_str_mv | Adenosine Dopamine Retinoid X receptor Serotonin Sleep |
| title | The retinoid X receptor: a nuclear receptor that modulates the sleep-wake cycle in rats |
| title_full | The retinoid X receptor: a nuclear receptor that modulates the sleep-wake cycle in rats |
| title_fullStr | The retinoid X receptor: a nuclear receptor that modulates the sleep-wake cycle in rats |
| title_full_unstemmed | The retinoid X receptor: a nuclear receptor that modulates the sleep-wake cycle in rats |
| title_short | The retinoid X receptor: a nuclear receptor that modulates the sleep-wake cycle in rats |
| title_sort | The retinoid X receptor: a nuclear receptor that modulates the sleep-wake cycle in rats |
| topic | Adenosine Dopamine Retinoid X receptor Serotonin Sleep |
| topic_facet | Adenosine Dopamine Retinoid X receptor Serotonin Sleep |
| url | http://hdl.handle.net/10451/53293 |
| visible | 1 |