Publicação
Reprogramming of rye rDNA in triticale during microsporogenesis
| Resumo: | To test the hypothesis that interspecific genomic and chromosome interactions leading to nucleolar dominance could be reprogrammed in meiosis, we compared the expression of distinct nucleolar organizing region (NOR) loci in hexaploid triticale root tip meristematic cells, pollen mother cells and young pollen grains. Interphase and metaphase cells were silver stained to quantify nucleoli and active NOR loci respectively. A marked difference in the ribosomal RNA gene activity of each locus was observed when different types of cells were compared: in somatic and pollen mother cells, rRNA gene activity was mainly restricted to major wheat NORs (1B and 6B) with only a small contribution from rye NORs (1R). In contrast, in young pollen grains, all NORs present, including the 1R NORs, were consistently active. The expression of all NORs just after meiosis is considered to be a consequence of meiotic reprogramming of rye origin rDNA. Gene reprogramming mediated by the resetting of methylation patterns established early in embryogenesis is suggested to be responsible for the differential expression of the NORs of rye origin in distinct developmental stages of triticale. |
|---|---|
| Autores principais: | Silva, M. |
| Outros Autores: | Queiroz, A.; Neves, N.; Barão, A.; Castilho, A.; Morais-Cecilio, L.; Viegas, W. |
| Assunto: | meiotic reprogramming microspores nucleolar dominance rDNA expression rye NOR |
| Ano: | 1995 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1866810414403158016 |
|---|---|
| author | Silva, M. |
| author2 | Queiroz, A. Neves, N. Barão, A. Castilho, A. Morais-Cecilio, L. Viegas, W. |
| author2_role | author author author author author author |
| author_facet | Silva, M. Queiroz, A. Neves, N. Barão, A. Castilho, A. Morais-Cecilio, L. Viegas, W. |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Silva, M.\"},{\"Person.name\":\"Queiroz, A.\"},{\"Person.name\":\"Neves, N.\"},{\"Person.name\":\"Barão, A.\"},{\"Person.name\":\"Castilho, A.\"},{\"Person.name\":\"Morais-Cecilio, L.\",\"Person.identifier.orcid\":\"0000-0001-9313-2253\"},{\"Person.name\":\"Viegas, W.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Silva, M. Queiroz, A. Neves, N. Barão, A. Castilho, A. Morais-Cecilio, L. Viegas, W. |
| datacite.date.Accepted.fl_str_mv | 1995-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2011-04-05T13:22:53Z |
| datacite.date.embargoed.fl_str_mv | 2011-04-05T13:22:53Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | meiotic reprogramming microspores nucleolar dominance rDNA expression rye NOR |
| datacite.titles.title.fl_str_mv | Reprogramming of rye rDNA in triticale during microsporogenesis |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Silva, M. Queiroz, A. Neves, N. Barão, A. Castilho, A. Morais-Cecilio, L. Viegas, W. |
| dc.date.Accepted.fl_str_mv | 1995-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2011-04-05T13:22:53Z |
| dc.date.embargoed.fl_str_mv | 2011-04-05T13:22:53Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.5/3047 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Springer |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | meiotic reprogramming microspores nucleolar dominance rDNA expression rye NOR |
| dc.title.fl_str_mv | Reprogramming of rye rDNA in triticale during microsporogenesis |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | To test the hypothesis that interspecific genomic and chromosome interactions leading to nucleolar dominance could be reprogrammed in meiosis, we compared the expression of distinct nucleolar organizing region (NOR) loci in hexaploid triticale root tip meristematic cells, pollen mother cells and young pollen grains. Interphase and metaphase cells were silver stained to quantify nucleoli and active NOR loci respectively. A marked difference in the ribosomal RNA gene activity of each locus was observed when different types of cells were compared: in somatic and pollen mother cells, rRNA gene activity was mainly restricted to major wheat NORs (1B and 6B) with only a small contribution from rye NORs (1R). In contrast, in young pollen grains, all NORs present, including the 1R NORs, were consistently active. The expression of all NORs just after meiosis is considered to be a consequence of meiotic reprogramming of rye origin rDNA. Gene reprogramming mediated by the resetting of methylation patterns established early in embryogenesis is suggested to be responsible for the differential expression of the NORs of rye origin in distinct developmental stages of triticale. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/979f5bf9-31cb-4f9f-a450-ebf2db12e6ad/download |
| id | ul_ace11313eeff71aaea0cd7f93d2efaae |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.5/3047 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10400.5/3047 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Silva, M. Queiroz, A. Neves, N. Barão, A. Castilho, A. Morais-Cecilio, L. Morais-Cecilio, L. https://www.ciencia-id.pt/B013-3032-6A83 B013-3032-6A83 http://orcid.org/0000-0001-9313-2253 0000-0001-9313-2253 Viegas, W. |
| publishDate | 1995 |
| publisher.none.fl_str_mv | Springer |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engSpringerporTo test the hypothesis that interspecific genomic and chromosome interactions leading to nucleolar dominance could be reprogrammed in meiosis, we compared the expression of distinct nucleolar organizing region (NOR) loci in hexaploid triticale root tip meristematic cells, pollen mother cells and young pollen grains. Interphase and metaphase cells were silver stained to quantify nucleoli and active NOR loci respectively. A marked difference in the ribosomal RNA gene activity of each locus was observed when different types of cells were compared: in somatic and pollen mother cells, rRNA gene activity was mainly restricted to major wheat NORs (1B and 6B) with only a small contribution from rye NORs (1R). In contrast, in young pollen grains, all NORs present, including the 1R NORs, were consistently active. The expression of all NORs just after meiosis is considered to be a consequence of meiotic reprogramming of rye origin rDNA. Gene reprogramming mediated by the resetting of methylation patterns established early in embryogenesis is suggested to be responsible for the differential expression of the NORs of rye origin in distinct developmental stages of triticale.application/pdfporReprogramming of rye rDNA in triticale during microsporogenesisSilva, M.Queiroz, A.Neves, N.Barão, A.Castilho, A.PersonalMorais-Cecilio, L.DSpacehttp://dspace.org/items/ecba15d2-48ef-458a-83ed-524894adef07DSpacehttp://dspace.org/items/ecba15d2-48ef-458a-83ed-524894adef07Morais-CecilioLeonorCiência IDhttps://www.ciencia-id.ptB013-3032-6A83ORCIDhttp://orcid.org0000-0001-9313-2253Scopus Author IDhttps://www.scopus.com8235554800Scopus Author IDhttps://www.scopus.com55958947400Viegas, W.HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptISSNIsPartOf0967-38492011-04-05T13:22:53Z19951995-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.5/3047http://purl.org/coar/access_right/c_abf2open accessmeiotic reprogrammingmicrosporesnucleolar dominancerDNA expressionrye NOR458609 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/979f5bf9-31cb-4f9f-a450-ebf2db12e6ad/downloadChromosome Research492496 |
| spellingShingle | Reprogramming of rye rDNA in triticale during microsporogenesis Silva, M. meiotic reprogramming microspores nucleolar dominance rDNA expression rye NOR |
| status | SINGLETON |
| subject.fl_str_mv | meiotic reprogramming microspores nucleolar dominance rDNA expression rye NOR |
| title | Reprogramming of rye rDNA in triticale during microsporogenesis |
| title_full | Reprogramming of rye rDNA in triticale during microsporogenesis |
| title_fullStr | Reprogramming of rye rDNA in triticale during microsporogenesis |
| title_full_unstemmed | Reprogramming of rye rDNA in triticale during microsporogenesis |
| title_short | Reprogramming of rye rDNA in triticale during microsporogenesis |
| title_sort | Reprogramming of rye rDNA in triticale during microsporogenesis |
| topic | meiotic reprogramming microspores nucleolar dominance rDNA expression rye NOR |
| topic_facet | meiotic reprogramming microspores nucleolar dominance rDNA expression rye NOR |
| url | http://hdl.handle.net/10400.5/3047 |
| visible | 1 |