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The use of metagenomic approaches by NGS-based 16S rRNA to analyse tick’s microbial communities

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Resumo:Ticks are ectoparasitic arthropods that feed exclusively on blood from a large range of hosts, including humans. These arthropods are included in families Argasidae, Ixodidae and Nuttalliellidae. They are of great importance in health, especially the Ixodidae, due to their capacity to transmit microorganisms, some of which may be responsible for high morbidity and mortality rates in domestic and wild animals and humans. For the present study, tick samples were collected from dogs and cats in veterinary clinics distributed throughout national territory, which constitute the FCISA - Tick Collection, deposited in the Department of Animal Biology of the Faculty of Sciences of the University of Lisbon. This work aims to evaluate tick species circulating in domestic dogs and cats (mainland Portugal) and their bacterial microbiome. Ticks were morphologically identified and confirmed by COI genetic marker. To understand the tick microbiome, eight DNA pools were created to perform metagenomic analysis by NGS-based 16S rRNA. Morphological and molecular identification of tick species were concordant, highlighting the presence of several species parasitizing dogs and cats. The 16S rRNA metagenomic results showed a high diversity of bacteria in the tick species studied, with "Candidatus Midichloria”, Coxiella, Francisella, Wolbachia and Pseudomonas being the most abundant bacterial genera. Other Bacteria associated with soil, water, plants, arthropods and vertebrates were identified in this study and have already been related to ticks, suggesting that these bacteria may be acquired from the environment and from host, which potentially result in variations in the tick microbiome. Many other minor genera Bacteria (<1%) have also been detected, which have not yet been associated with ticks and whose importance is unknown. This study demonstrated which Bacteria are present in the microbiome of the most abundant ticks in Portugal, but it can be improved with further studies.
Autores principais:Silva, Diogo Alexandre Martins da
Assunto:Carraças Metagenómica Agentes Patogénicos Microbioma 16S rRNA Teses de mestrado - 2025
Ano:2025
País:Portugal
Tipo de documento:dissertação de mestrado
Tipo de acesso:acesso embargado
Instituição associada:Universidade de Lisboa
Idioma:inglês
Origem:Repositório da Universidade de Lisboa

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