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Durum wheat diversity for heat stress tolerance during inflorescence emergence is correlated to TdHSP101C expression in early developmental stages

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Resumo:The predicted world population increase along with climate changes threatens sustainable agricultural supply in the coming decades. It is therefore vital to understand crops diversity associated to abiotic stress response. Heat stress is considered one of the major constrains on crops productivity thus it is essential to develop new approaches for a precocious and rigorous evaluation of varietal diversity regarding heat tolerance. Plant cell membrane thermostability (CMS) is a widely used method for wheat thermotolerance assessment although its limitations require complementary solutions. In this work we used CMS assay and explored TdHSP101C genes as an additional tool for durum wheat screening. Genomic and transcriptomic analyses of TdHSP101C genes were performed in varieties with contrasting CMS results and further correlated with heat stress tolerance during fertilization and seed development. Although the durum wheat varieties studied presented a very high homology on TdHSP101C genes (>99%) the transcriptomic assessment allowed the discrimination between varieties with good CMS results and its correlation with differential impacts of heat treatment during inflorescence emergence and seed development on grain yield. The evidences here reported indicate that TdHSP101C transcription levels induced by heat stress in fully expanded leaves may be a promising complementary screening tool to discriminate between durum wheat varieties identified as thermotolerant through CMS
Autores principais:Bento, Miguel
Outros Autores:Pereira, Sónia Gomes; Viegas, Wanda; Silva, Manuela
Assunto:durum wheat Triticum durum heat stress inflorescence emergence
Ano:2017
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade de Lisboa
Idioma:inglês
Origem:Repositório da Universidade de Lisboa
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author Bento, Miguel
author2 Pereira, Sónia Gomes
Viegas, Wanda
Silva, Manuela
author2_role author
author
author
author_facet Bento, Miguel
Pereira, Sónia Gomes
Viegas, Wanda
Silva, Manuela
author_role author
contributor_name_str_mv Repositório Científico de Acesso Aberto da ULisboa
country_str PT
creators_json_txt [{\"Person.name\":\"Bento, Miguel\"},{\"Person.name\":\"Pereira, Sónia Gomes\"},{\"Person.name\":\"Viegas, Wanda\"},{\"Person.name\":\"Silva, Manuela\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Repositório Científico de Acesso Aberto da ULisboa
datacite.creators.creator.creatorName.fl_str_mv Bento, Miguel
Pereira, Sónia Gomes
Viegas, Wanda
Silva, Manuela
datacite.date.Accepted.fl_str_mv 2017-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2018-01-02T10:23:44Z
datacite.date.embargoed.fl_str_mv 2018-01-02T10:23:44Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv durum wheat
Triticum durum
heat stress
inflorescence emergence
datacite.titles.title.fl_str_mv Durum wheat diversity for heat stress tolerance during inflorescence emergence is correlated to TdHSP101C expression in early developmental stages
dc.contributor.none.fl_str_mv Repositório Científico de Acesso Aberto da ULisboa
dc.creator.none.fl_str_mv Bento, Miguel
Pereira, Sónia Gomes
Viegas, Wanda
Silva, Manuela
dc.date.Accepted.fl_str_mv 2017-01-01T00:00:00Z
dc.date.available.fl_str_mv 2018-01-02T10:23:44Z
dc.date.embargoed.fl_str_mv 2018-01-02T10:23:44Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10400.5/14589
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv Guangxiao Yang (ed.)
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.subject.none.fl_str_mv durum wheat
Triticum durum
heat stress
inflorescence emergence
dc.title.fl_str_mv Durum wheat diversity for heat stress tolerance during inflorescence emergence is correlated to TdHSP101C expression in early developmental stages
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description The predicted world population increase along with climate changes threatens sustainable agricultural supply in the coming decades. It is therefore vital to understand crops diversity associated to abiotic stress response. Heat stress is considered one of the major constrains on crops productivity thus it is essential to develop new approaches for a precocious and rigorous evaluation of varietal diversity regarding heat tolerance. Plant cell membrane thermostability (CMS) is a widely used method for wheat thermotolerance assessment although its limitations require complementary solutions. In this work we used CMS assay and explored TdHSP101C genes as an additional tool for durum wheat screening. Genomic and transcriptomic analyses of TdHSP101C genes were performed in varieties with contrasting CMS results and further correlated with heat stress tolerance during fertilization and seed development. Although the durum wheat varieties studied presented a very high homology on TdHSP101C genes (>99%) the transcriptomic assessment allowed the discrimination between varieties with good CMS results and its correlation with differential impacts of heat treatment during inflorescence emergence and seed development on grain yield. The evidences here reported indicate that TdHSP101C transcription levels induced by heat stress in fully expanded leaves may be a promising complementary screening tool to discriminate between durum wheat varieties identified as thermotolerant through CMS
dirty 0
eu_rights_str_mv openAccess
format article
fulltext.url.fl_str_mv https://repositorio.ulisboa.pt/bitstreams/13ed6971-eb11-4415-8962-ffe32ebcab3d/download
funding.funder.alternateName_str_mv FCT
funding.funder.identifier_str_mv http://doi.org/10.13039/501100001871
funding.funder.name_str_mv Fundação para a Ciência e a Tecnologia
id ul_bd05fbfff3968aaddd4e2a4e209824ae
identifier.url.fl_str_mv http://hdl.handle.net/10400.5/14589
instacron_str ul
institution Universidade de Lisboa
instname_str Universidade de Lisboa
language eng
network_acronym_str ul
network_name_str Repositório da Universidade de Lisboa
oai_identifier_str oai:repositorio.ulisboa.pt:10400.5/14589
organization_str_mv urn:organizationAcronym:ul
person_str_mv Bento, Miguel
Pereira, Sónia Gomes
Viegas, Wanda
Silva, Manuela
publishDate 2017
publisher.none.fl_str_mv Guangxiao Yang (ed.)
reponame_str Repositório da Universidade de Lisboa
repository_id_str urn:repositoryAcronym:ul
service_str_mv urn:repositoryAcronym:ul
spelling engGuangxiao Yang (ed.)pt_PTThe predicted world population increase along with climate changes threatens sustainable agricultural supply in the coming decades. It is therefore vital to understand crops diversity associated to abiotic stress response. Heat stress is considered one of the major constrains on crops productivity thus it is essential to develop new approaches for a precocious and rigorous evaluation of varietal diversity regarding heat tolerance. Plant cell membrane thermostability (CMS) is a widely used method for wheat thermotolerance assessment although its limitations require complementary solutions. In this work we used CMS assay and explored TdHSP101C genes as an additional tool for durum wheat screening. Genomic and transcriptomic analyses of TdHSP101C genes were performed in varieties with contrasting CMS results and further correlated with heat stress tolerance during fertilization and seed development. Although the durum wheat varieties studied presented a very high homology on TdHSP101C genes (>99%) the transcriptomic assessment allowed the discrimination between varieties with good CMS results and its correlation with differential impacts of heat treatment during inflorescence emergence and seed development on grain yield. The evidences here reported indicate that TdHSP101C transcription levels induced by heat stress in fully expanded leaves may be a promising complementary screening tool to discriminate between durum wheat varieties identified as thermotolerant through CMSapplication/pdfpt_PTDurum wheat diversity for heat stress tolerance during inflorescence emergence is correlated to TdHSP101C expression in early developmental stagesBento, MiguelPereira, Sónia GomesViegas, WandaSilva, ManuelaHostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptDOIIsPartOfhttps://doi.org/10.1371/journal. pone.01900852018-01-02T10:23:44Z20172017-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.5/14589http://purl.org/coar/access_right/c_abf2open accessdurum wheatTriticum durumheat stressinflorescence emergence1176599 bytesFundação para a Ciência e a TecnologiaSUSTAINED ENRICHMENT OF TRITICUM DURUM BIODIVERSITY POOL: DISCLOSURE OF MOLECULAR MARKERS ASSOCIATED TO EXTREME TEMPERATURE AND ACID SOIL TOLERANCECrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/13ed6971-eb11-4415-8962-ffe32ebcab3d/downloadPLoS ONE
spellingShingle Durum wheat diversity for heat stress tolerance during inflorescence emergence is correlated to TdHSP101C expression in early developmental stages
Bento, Miguel
durum wheat
Triticum durum
heat stress
inflorescence emergence
status SINGLETON
subject.fl_str_mv durum wheat
Triticum durum
heat stress
inflorescence emergence
title Durum wheat diversity for heat stress tolerance during inflorescence emergence is correlated to TdHSP101C expression in early developmental stages
title_full Durum wheat diversity for heat stress tolerance during inflorescence emergence is correlated to TdHSP101C expression in early developmental stages
title_fullStr Durum wheat diversity for heat stress tolerance during inflorescence emergence is correlated to TdHSP101C expression in early developmental stages
title_full_unstemmed Durum wheat diversity for heat stress tolerance during inflorescence emergence is correlated to TdHSP101C expression in early developmental stages
title_short Durum wheat diversity for heat stress tolerance during inflorescence emergence is correlated to TdHSP101C expression in early developmental stages
title_sort Durum wheat diversity for heat stress tolerance during inflorescence emergence is correlated to TdHSP101C expression in early developmental stages
topic durum wheat
Triticum durum
heat stress
inflorescence emergence
topic_facet durum wheat
Triticum durum
heat stress
inflorescence emergence
url http://hdl.handle.net/10400.5/14589
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