Publicação
Antibiotic resistance and virulence profiles of Gram-Negative bacteria isolated from loggerhead sea turtles (Caretta caretta) of the Island of Maio, Cape Verde
| Resumo: | Previous studies revealed high levels of antimicrobial resistance (AMR) in loggerhead sea turtles (Caretta caretta), describing this species as prime reservoir of antimicrobial-resistant bacteria. This study aimed to characterise, for the first time, the AMR and virulence profiles of Gram-negative bacteria isolated from 33 nesting loggerhead turtles of the island of Maio, Cape Verde. Cloacal, oral, and egg content swab samples (n = 99) were collected and analysed using conventional bacteriological techniques. Shewanella putrefaciens, Morganella morganii, and Vibrio alginolyticus were isolated from the samples under study. The isolates obtained from this loggerhead subpopulation (North-East Atlantic) revealed lower levels of AMR, compared with the results of studies performed in other subpopulations (e.g., Mediterranean). However, the detection of resistance to carbapenems and multiple antimicrobial resistance indices higher than 0.20, raises concern about the potential association of these animals to points of high antimicrobial exposure. Furthermore, virulence phenotypic characterisation revealed that the isolates presented complex virulence profiles, including the ability to produce biofilms. Finally, due to their pathogenic potential, and considering the evidence of illegal consumption of turtle-related products on the island of Maio, the identified bacteria may represent a significant threat to public health. |
|---|---|
| Autores principais: | Fernandes, Matilde |
| Outros Autores: | Grilo, Miguel; Cunha, Eva; Carneiro, Carla; Tavares, Luis; Patino-Martinez, Juan; Oliveira, Manuela |
| Assunto: | Caretta caretta Island of Maio Antimicrobial resistance Bacterial virulence factors One Health |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1866809072921083904 |
|---|---|
| author | Fernandes, Matilde |
| author2 | Grilo, Miguel Cunha, Eva Carneiro, Carla Tavares, Luis Patino-Martinez, Juan Oliveira, Manuela |
| author2_role | author author author author author author |
| author_facet | Fernandes, Matilde Grilo, Miguel Cunha, Eva Carneiro, Carla Tavares, Luis Patino-Martinez, Juan Oliveira, Manuela |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Fernandes, Matilde\"},{\"Person.name\":\"Grilo, Miguel\",\"Person.identifier.orcid\":\"0000-0001-7364-821X\"},{\"Person.name\":\"Cunha, Eva\",\"Person.identifier.orcid\":\"0000-0002-5641-4400\"},{\"Person.name\":\"Carneiro, Carla\"},{\"Person.name\":\"Tavares, Luis\",\"Person.identifier.orcid\":\"0000-0001-8671-6285\"},{\"Person.name\":\"Patino-Martinez, Juan\"},{\"Person.name\":\"Oliveira, Manuela\",\"Person.identifier.orcid\":\"0000-0002-3910-1525\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Fernandes, Matilde Grilo, Miguel Cunha, Eva Carneiro, Carla Tavares, Luis Patino-Martinez, Juan Oliveira, Manuela |
| datacite.date.Accepted.fl_str_mv | 2021-06-24T00:00:00Z |
| datacite.date.available.fl_str_mv | 2021-11-08T17:43:26Z |
| datacite.date.embargoed.fl_str_mv | 2021-11-08T17:43:26Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Caretta caretta Island of Maio Antimicrobial resistance Bacterial virulence factors One Health |
| datacite.titles.title.fl_str_mv | Antibiotic resistance and virulence profiles of Gram-Negative bacteria isolated from loggerhead sea turtles (Caretta caretta) of the Island of Maio, Cape Verde |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Fernandes, Matilde Grilo, Miguel Cunha, Eva Carneiro, Carla Tavares, Luis Patino-Martinez, Juan Oliveira, Manuela |
| dc.date.Accepted.fl_str_mv | 2021-06-24T00:00:00Z |
| dc.date.available.fl_str_mv | 2021-11-08T17:43:26Z |
| dc.date.embargoed.fl_str_mv | 2021-11-08T17:43:26Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.5/22528 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Caretta caretta Island of Maio Antimicrobial resistance Bacterial virulence factors One Health |
| dc.title.fl_str_mv | Antibiotic resistance and virulence profiles of Gram-Negative bacteria isolated from loggerhead sea turtles (Caretta caretta) of the Island of Maio, Cape Verde |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Previous studies revealed high levels of antimicrobial resistance (AMR) in loggerhead sea turtles (Caretta caretta), describing this species as prime reservoir of antimicrobial-resistant bacteria. This study aimed to characterise, for the first time, the AMR and virulence profiles of Gram-negative bacteria isolated from 33 nesting loggerhead turtles of the island of Maio, Cape Verde. Cloacal, oral, and egg content swab samples (n = 99) were collected and analysed using conventional bacteriological techniques. Shewanella putrefaciens, Morganella morganii, and Vibrio alginolyticus were isolated from the samples under study. The isolates obtained from this loggerhead subpopulation (North-East Atlantic) revealed lower levels of AMR, compared with the results of studies performed in other subpopulations (e.g., Mediterranean). However, the detection of resistance to carbapenems and multiple antimicrobial resistance indices higher than 0.20, raises concern about the potential association of these animals to points of high antimicrobial exposure. Furthermore, virulence phenotypic characterisation revealed that the isolates presented complex virulence profiles, including the ability to produce biofilms. Finally, due to their pathogenic potential, and considering the evidence of illegal consumption of turtle-related products on the island of Maio, the identified bacteria may represent a significant threat to public health. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/0211b90f-2a55-4ffd-8455-1b1e210b200a/download |
| id | ul_bd8a3d7fd329e2863e1ec55368d4bd8d |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.5/22528 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10400.5/22528 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Fernandes, Matilde Grilo, Miguel Grilo, Miguel https://www.ciencia-id.pt/4B17-DF6F-1A51 4B17-DF6F-1A51 http://orcid.org/0000-0001-7364-821X 0000-0001-7364-821X Cunha, Eva Cunha, Eva https://www.ciencia-id.pt/9811-7B6B-E2B9 9811-7B6B-E2B9 http://orcid.org/0000-0002-5641-4400 0000-0002-5641-4400 Carneiro, Carla Carneiro, Carla https://www.ciencia-id.pt/F817-1D4D-8411 F817-1D4D-8411 Tavares, Luis Tavares, Luis https://www.ciencia-id.pt/9011-18C6-482D 9011-18C6-482D http://orcid.org/0000-0001-8671-6285 0000-0001-8671-6285 Patino-Martinez, Juan Oliveira, Manuela Oliveira, Manuela https://www.ciencia-id.pt/BE1E-2B3B-92D2 BE1E-2B3B-92D2 http://orcid.org/0000-0002-3910-1525 0000-0002-3910-1525 |
| publishDate | 2021 |
| publisher.none.fl_str_mv | MDPI |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engMDPIpt_PTPrevious studies revealed high levels of antimicrobial resistance (AMR) in loggerhead sea turtles (Caretta caretta), describing this species as prime reservoir of antimicrobial-resistant bacteria. This study aimed to characterise, for the first time, the AMR and virulence profiles of Gram-negative bacteria isolated from 33 nesting loggerhead turtles of the island of Maio, Cape Verde. Cloacal, oral, and egg content swab samples (n = 99) were collected and analysed using conventional bacteriological techniques. Shewanella putrefaciens, Morganella morganii, and Vibrio alginolyticus were isolated from the samples under study. The isolates obtained from this loggerhead subpopulation (North-East Atlantic) revealed lower levels of AMR, compared with the results of studies performed in other subpopulations (e.g., Mediterranean). However, the detection of resistance to carbapenems and multiple antimicrobial resistance indices higher than 0.20, raises concern about the potential association of these animals to points of high antimicrobial exposure. Furthermore, virulence phenotypic characterisation revealed that the isolates presented complex virulence profiles, including the ability to produce biofilms. Finally, due to their pathogenic potential, and considering the evidence of illegal consumption of turtle-related products on the island of Maio, the identified bacteria may represent a significant threat to public health.application/pdfpt_PTAntibiotic resistance and virulence profiles of Gram-Negative bacteria isolated from loggerhead sea turtles (Caretta caretta) of the Island of Maio, Cape VerdeFernandes, MatildePersonalGrilo, MiguelDSpacehttp://dspace.org/items/1f136cc1-1f56-40b3-971b-aab3edaa7cabDSpacehttp://dspace.org/items/1f136cc1-1f56-40b3-971b-aab3edaa7cabGriloMiguelCiência IDhttps://www.ciencia-id.pt4B17-DF6F-1A51ORCIDhttp://orcid.org0000-0001-7364-821XScopus Author IDhttps://www.scopus.com57195729611PersonalCunha, EvaDSpacehttp://dspace.org/items/6177b175-6c2b-4b46-b383-f521ae7880ccDSpacehttp://dspace.org/items/6177b175-6c2b-4b46-b383-f521ae7880ccGonçalves da CunhaEva SofiaCiência IDhttps://www.ciencia-id.pt9811-7B6B-E2B9ORCIDhttp://orcid.org0000-0002-5641-4400PersonalCarneiro, CarlaDSpacehttp://dspace.org/items/24550fc3-f1f8-4dd4-82b1-599263055a57DSpacehttp://dspace.org/items/24550fc3-f1f8-4dd4-82b1-599263055a57Nóbrega CarneiroCarla VanessaCiência IDhttps://www.ciencia-id.ptF817-1D4D-8411PersonalTavares, LuisDSpacehttp://dspace.org/items/1afd8082-0715-4fa2-8314-d2d7de3fa937DSpacehttp://dspace.org/items/1afd8082-0715-4fa2-8314-d2d7de3fa937Morgado TavaresLuis ManuelCiência IDhttps://www.ciencia-id.pt9011-18C6-482DORCIDhttp://orcid.org0000-0001-8671-6285Patino-Martinez, JuanPersonalOliveira, ManuelaDSpacehttp://dspace.org/items/abd012d1-0b2c-43f6-ba34-5f65c785a479DSpacehttp://dspace.org/items/abd012d1-0b2c-43f6-ba34-5f65c785a479OliveiraManuelaCiência IDhttps://www.ciencia-id.ptBE1E-2B3B-92D2ORCIDhttp://orcid.org0000-0002-3910-1525HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptISSNIsPartOf2079-6382DOIIsPartOf10.3390/antibiotics100707712021-11-08T17:43:26Z2021-06-242021-06-24T00:00:00ZHandlehttp://hdl.handle.net/10400.5/22528http://purl.org/coar/access_right/c_abf2open accessCaretta carettaIsland of MaioAntimicrobial resistanceBacterial virulence factorsOne Health832768 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2021-06-24http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/0211b90f-2a55-4ffd-8455-1b1e210b200a/downloadAntibiotics771Basel, SWITZERLAND |
| spellingShingle | Antibiotic resistance and virulence profiles of Gram-Negative bacteria isolated from loggerhead sea turtles (Caretta caretta) of the Island of Maio, Cape Verde Fernandes, Matilde Caretta caretta Island of Maio Antimicrobial resistance Bacterial virulence factors One Health |
| status | SINGLETON |
| subject.fl_str_mv | Caretta caretta Island of Maio Antimicrobial resistance Bacterial virulence factors One Health |
| title | Antibiotic resistance and virulence profiles of Gram-Negative bacteria isolated from loggerhead sea turtles (Caretta caretta) of the Island of Maio, Cape Verde |
| title_full | Antibiotic resistance and virulence profiles of Gram-Negative bacteria isolated from loggerhead sea turtles (Caretta caretta) of the Island of Maio, Cape Verde |
| title_fullStr | Antibiotic resistance and virulence profiles of Gram-Negative bacteria isolated from loggerhead sea turtles (Caretta caretta) of the Island of Maio, Cape Verde |
| title_full_unstemmed | Antibiotic resistance and virulence profiles of Gram-Negative bacteria isolated from loggerhead sea turtles (Caretta caretta) of the Island of Maio, Cape Verde |
| title_short | Antibiotic resistance and virulence profiles of Gram-Negative bacteria isolated from loggerhead sea turtles (Caretta caretta) of the Island of Maio, Cape Verde |
| title_sort | Antibiotic resistance and virulence profiles of Gram-Negative bacteria isolated from loggerhead sea turtles (Caretta caretta) of the Island of Maio, Cape Verde |
| topic | Caretta caretta Island of Maio Antimicrobial resistance Bacterial virulence factors One Health |
| topic_facet | Caretta caretta Island of Maio Antimicrobial resistance Bacterial virulence factors One Health |
| url | http://hdl.handle.net/10400.5/22528 |
| visible | 1 |