Publicação
The interplay between membrane lipids and phospholipase A family members in grapevine resistance against Plasmopara viticola
| Resumo: | Grapevine downy mildew, caused by the biotrophic oomycete Plasmopara viticola, is one of the most important diseases in modern viticulture. The search for sustainable disease control measure is of extreme importance, thus becoming imperative to fully characterize the mechanisms leading to an incompatible interaction. We have previously shown that lipid signalling events play an important role in grapevine's response to this pathogen, namely through changes in linolenic acid content, lipid peroxidation and jasmonic acid synthesis. Here, we have characterized the modulation of lipid metabolism in leaves from two V. vinifera cultivars (resistant and susceptible to P. viticola) in the first hours after pathogen inoculation. Prior to pathogen inoculation both genotypes present an inherently different fatty acid composition that is highly modulated in the resistant genotype after pathogen challenge. Such changes involve modulation of phospholipase A activity suggesting that the source of lipids mobilized upon pathogen infection are the chloroplast membranes. This work thus provides original evidence on the involvement of lipid signalling and phospholipases in grapevine immune responses to pathogen infection. The results are discussed considering the implications on the plant's physiological status and the use of discriminating lipid/fatty acids pattern in future selection procedures of cultivars. |
|---|---|
| Autores principais: | Laureano, Gonçalo |
| Outros Autores: | Figueiredo, Joana; Cavaco, Ana Rita; Duarte, Bernardo; Caçador, Isabel; Malhó, Rui; Sousa Silva, Marta; Matos, Ana Rita; Figueiredo, Andreia |
| Ano: | 2018 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1868467332237492224 |
|---|---|
| author | Laureano, Gonçalo |
| author2 | Figueiredo, Joana Cavaco, Ana Rita Duarte, Bernardo Caçador, Isabel Malhó, Rui Sousa Silva, Marta Matos, Ana Rita Figueiredo, Andreia |
| author2_role | author author author author author author author author |
| author_facet | Laureano, Gonçalo Figueiredo, Joana Cavaco, Ana Rita Duarte, Bernardo Caçador, Isabel Malhó, Rui Sousa Silva, Marta Matos, Ana Rita Figueiredo, Andreia |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Laureano, Gonçalo\"},{\"Person.name\":\"Figueiredo, Joana\"},{\"Person.name\":\"Cavaco, Ana Rita\"},{\"Person.name\":\"Duarte, Bernardo\",\"Person.identifier.orcid\":\"0000-0003-1914-7435\"},{\"Person.name\":\"Caçador, Isabel\",\"Person.identifier.orcid\":\"0000-0002-4475-6091\"},{\"Person.name\":\"Malhó, Rui\"},{\"Person.name\":\"Sousa Silva, Marta\"},{\"Person.name\":\"Matos, Ana Rita\"},{\"Person.name\":\"Figueiredo, Andreia\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Laureano, Gonçalo Figueiredo, Joana Cavaco, Ana Rita Duarte, Bernardo Caçador, Isabel Malhó, Rui Sousa Silva, Marta Matos, Ana Rita Figueiredo, Andreia |
| datacite.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2019-06-25T09:44:59Z |
| datacite.date.embargoed.fl_str_mv | 2019-06-25T09:44:59Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.titles.title.fl_str_mv | The interplay between membrane lipids and phospholipase A family members in grapevine resistance against Plasmopara viticola |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Laureano, Gonçalo Figueiredo, Joana Cavaco, Ana Rita Duarte, Bernardo Caçador, Isabel Malhó, Rui Sousa Silva, Marta Matos, Ana Rita Figueiredo, Andreia |
| dc.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2019-06-25T09:44:59Z |
| dc.date.embargoed.fl_str_mv | 2019-06-25T09:44:59Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10451/38791 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.title.fl_str_mv | The interplay between membrane lipids and phospholipase A family members in grapevine resistance against Plasmopara viticola |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Grapevine downy mildew, caused by the biotrophic oomycete Plasmopara viticola, is one of the most important diseases in modern viticulture. The search for sustainable disease control measure is of extreme importance, thus becoming imperative to fully characterize the mechanisms leading to an incompatible interaction. We have previously shown that lipid signalling events play an important role in grapevine's response to this pathogen, namely through changes in linolenic acid content, lipid peroxidation and jasmonic acid synthesis. Here, we have characterized the modulation of lipid metabolism in leaves from two V. vinifera cultivars (resistant and susceptible to P. viticola) in the first hours after pathogen inoculation. Prior to pathogen inoculation both genotypes present an inherently different fatty acid composition that is highly modulated in the resistant genotype after pathogen challenge. Such changes involve modulation of phospholipase A activity suggesting that the source of lipids mobilized upon pathogen infection are the chloroplast membranes. This work thus provides original evidence on the involvement of lipid signalling and phospholipases in grapevine immune responses to pathogen infection. The results are discussed considering the implications on the plant's physiological status and the use of discriminating lipid/fatty acids pattern in future selection procedures of cultivars. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/61819aa2-d7c6-450d-9d67-f9e64126f8fe/download |
| id | ul_bd992f97a28db8bc85df7abb0971b0d7 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10451/38791 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
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| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10451/38791 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Laureano, Gonçalo Figueiredo, Joana Cavaco, Ana Rita Duarte, Bernardo Duarte, Bernardo https://www.ciencia-id.pt/731E-093F-D4C8 731E-093F-D4C8 http://orcid.org/0000-0003-1914-7435 0000-0003-1914-7435 Caçador, Isabel Caçador, Isabel https://www.ciencia-id.pt/631C-9FFE-CA81 631C-9FFE-CA81 http://orcid.org/0000-0002-4475-6091 0000-0002-4475-6091 Malhó, Rui Sousa Silva, Marta Matos, Ana Rita Figueiredo, Andreia |
| publishDate | 2018 |
| reponame_str | Repositório da Universidade de Lisboa |
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| spelling | engpt_PTGrapevine downy mildew, caused by the biotrophic oomycete Plasmopara viticola, is one of the most important diseases in modern viticulture. The search for sustainable disease control measure is of extreme importance, thus becoming imperative to fully characterize the mechanisms leading to an incompatible interaction. We have previously shown that lipid signalling events play an important role in grapevine's response to this pathogen, namely through changes in linolenic acid content, lipid peroxidation and jasmonic acid synthesis. Here, we have characterized the modulation of lipid metabolism in leaves from two V. vinifera cultivars (resistant and susceptible to P. viticola) in the first hours after pathogen inoculation. Prior to pathogen inoculation both genotypes present an inherently different fatty acid composition that is highly modulated in the resistant genotype after pathogen challenge. Such changes involve modulation of phospholipase A activity suggesting that the source of lipids mobilized upon pathogen infection are the chloroplast membranes. This work thus provides original evidence on the involvement of lipid signalling and phospholipases in grapevine immune responses to pathogen infection. The results are discussed considering the implications on the plant's physiological status and the use of discriminating lipid/fatty acids pattern in future selection procedures of cultivars.application/pdfpt_PTThe interplay between membrane lipids and phospholipase A family members in grapevine resistance against Plasmopara viticolaLaureano, GonçaloFigueiredo, JoanaCavaco, Ana RitaPersonalDuarte, BernardoDSpacehttp://dspace.org/items/a72f0f40-c224-49ad-ba21-ebb5f9aab09cDSpacehttp://dspace.org/items/a72f0f40-c224-49ad-ba21-ebb5f9aab09cDuarteBernardoCiência IDhttps://www.ciencia-id.pt731E-093F-D4C8ORCIDhttp://orcid.org0000-0003-1914-7435Researcher IDhttps://www.researcherid.comH-2001-2011Scopus Author IDhttps://www.scopus.com20734149900PersonalCaçador, IsabelDSpacehttp://dspace.org/items/02ecb390-88b9-4e2f-8690-eba66c3b5311DSpacehttp://dspace.org/items/02ecb390-88b9-4e2f-8690-eba66c3b5311CaçadorIsabelCiência IDhttps://www.ciencia-id.pt631C-9FFE-CA81ORCIDhttp://orcid.org0000-0002-4475-6091Researcher IDhttps://www.researcherid.comC-2618-2012Scopus Author IDhttps://www.scopus.com6602533871Scopus Author IDhttps://www.scopus.com55126637700Malhó, RuiSousa Silva, MartaMatos, Ana RitaFigueiredo, AndreiaHostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptISSNIsPartOf2045-2322DOIIsPartOf10.1038/s41598-018-32559-z2019-06-25T09:44:59Z20182018-01-01T00:00:00ZHandlehttp://hdl.handle.net/10451/38791http://purl.org/coar/access_right/c_16ecrestricted access3602902 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/61819aa2-d7c6-450d-9d67-f9e64126f8fe/downloadScientific Reports8114538 |
| spellingShingle | The interplay between membrane lipids and phospholipase A family members in grapevine resistance against Plasmopara viticola Laureano, Gonçalo |
| status | SINGLETON |
| title | The interplay between membrane lipids and phospholipase A family members in grapevine resistance against Plasmopara viticola |
| title_full | The interplay between membrane lipids and phospholipase A family members in grapevine resistance against Plasmopara viticola |
| title_fullStr | The interplay between membrane lipids and phospholipase A family members in grapevine resistance against Plasmopara viticola |
| title_full_unstemmed | The interplay between membrane lipids and phospholipase A family members in grapevine resistance against Plasmopara viticola |
| title_short | The interplay between membrane lipids and phospholipase A family members in grapevine resistance against Plasmopara viticola |
| title_sort | The interplay between membrane lipids and phospholipase A family members in grapevine resistance against Plasmopara viticola |
| url | http://hdl.handle.net/10451/38791 |
| visible | 1 |