Publicação
Lignin composition and structure differs between xylem, phloem and phellem in Quercus suber
| Resumo: | The composition and structure of lignin in different tissues—phellem (cork), phloem and xylem (wood)—of Quercus suber was studied. Whole cell walls and their respective isolated milled lignins were analyzed by pyrolysis coupled with gas chromatography/mass spectrometry (Py-GC/MS), two-dimensional nuclear magnetic resonance spectroscopy (2D-NMR) and derivatization followed by reductive cleavage (DFRC). Different tissues presented varied p-hydroxyphenyl:guaiacyl:syringyl (H:G:S) lignin compositions. Whereas lignin from cork has a G-rich lignin (H:G:S molar ratio 2:85:13), lignin from phloem presents more S-units (H:G:S molar ratio of 1:58:41) and lignin from xylem is slightly enriched in S-lignin (H:G:S molar ratio 1:45:55). These differences were reflected in the relative abundances of the different interunit linkages. Alkyl-aryl ethers (β–O–4′) were predominant, increasing from 68% in cork, to 71% in phloem and 77% in xylem, as consequence of the enrichment in S-lignin units. Cork lignin was enriched in condensed structures such as phenylcoumarans (β-5′, 20%), dibenzodioxocins (5–5′, 5%), as corresponds to a lignin enriched in G-units. In comparison, lignin from phloem and xylem presented lower levels of condensed linkages. The lignin from cork was highly acetylated at the γ-OH of the side-chain (48% lignin acetylation), predominantly over G-units; while the lignins from phloem and xylem were barely acetylated and this occurred mainly over S-units. These results are a first time overview of the lignin structure in xylem, phloem (generated by cambium), and in cork (generated by phellogen), in agreement with literature that reports that lignin biosynthesis is flexible and cell specific. |
|---|---|
| Autores principais: | Lourenço, Ana |
| Outros Autores: | Rencoret, Jorge; Chemetova, Catarina; Gominho, Jorge; Gutierrez, Ana; Rio, José C. del; Pereira, Helena |
| Assunto: | cork DFRC milled lignin NMR phloem Py-GC/MS Quercus suber xylem |
| Ano: | 2016 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1865920832003375104 |
|---|---|
| author | Lourenço, Ana |
| author2 | Rencoret, Jorge Chemetova, Catarina Gominho, Jorge Gutierrez, Ana Rio, José C. del Pereira, Helena |
| author2_role | author author author author author author |
| author_facet | Lourenço, Ana Lourenço, Ana Rencoret, Jorge Chemetova, Catarina Gominho, Jorge Gutierrez, Ana Rio, José C. del Pereira, Helena Rencoret, Jorge Chemetova, Catarina Gominho, Jorge Gutierrez, Ana Rio, José C. del Pereira, Helena |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Lourenço, Ana\"},{\"Person.name\":\"Rencoret, Jorge\"},{\"Person.name\":\"Chemetova, Catarina\"},{\"Person.name\":\"Gominho, Jorge\"},{\"Person.name\":\"Gutierrez, Ana\"},{\"Person.name\":\"Rio, José C. del\"},{\"Person.name\":\"Pereira, Helena\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Lourenço, Ana Rencoret, Jorge Chemetova, Catarina Gominho, Jorge Gutierrez, Ana Rio, José C. del Pereira, Helena |
| datacite.date.Accepted.fl_str_mv | 2016-10-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2017-06-09T10:25:41Z |
| datacite.date.embargoed.fl_str_mv | 2017-06-09T10:25:41Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | cork DFRC milled lignin NMR phloem Py-GC/MS Quercus suber xylem |
| datacite.titles.title.fl_str_mv | Lignin composition and structure differs between xylem, phloem and phellem in Quercus suber |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Lourenço, Ana Rencoret, Jorge Chemetova, Catarina Gominho, Jorge Gutierrez, Ana Rio, José C. del Pereira, Helena |
| dc.date.Accepted.fl_str_mv | 2016-10-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2017-06-09T10:25:41Z |
| dc.date.embargoed.fl_str_mv | 2017-06-09T10:25:41Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.5/13741 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Danièle Werk (ed.) |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | cork DFRC milled lignin NMR phloem Py-GC/MS Quercus suber xylem |
| dc.title.fl_str_mv | Lignin composition and structure differs between xylem, phloem and phellem in Quercus suber |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The composition and structure of lignin in different tissues—phellem (cork), phloem and xylem (wood)—of Quercus suber was studied. Whole cell walls and their respective isolated milled lignins were analyzed by pyrolysis coupled with gas chromatography/mass spectrometry (Py-GC/MS), two-dimensional nuclear magnetic resonance spectroscopy (2D-NMR) and derivatization followed by reductive cleavage (DFRC). Different tissues presented varied p-hydroxyphenyl:guaiacyl:syringyl (H:G:S) lignin compositions. Whereas lignin from cork has a G-rich lignin (H:G:S molar ratio 2:85:13), lignin from phloem presents more S-units (H:G:S molar ratio of 1:58:41) and lignin from xylem is slightly enriched in S-lignin (H:G:S molar ratio 1:45:55). These differences were reflected in the relative abundances of the different interunit linkages. Alkyl-aryl ethers (β–O–4′) were predominant, increasing from 68% in cork, to 71% in phloem and 77% in xylem, as consequence of the enrichment in S-lignin units. Cork lignin was enriched in condensed structures such as phenylcoumarans (β-5′, 20%), dibenzodioxocins (5–5′, 5%), as corresponds to a lignin enriched in G-units. In comparison, lignin from phloem and xylem presented lower levels of condensed linkages. The lignin from cork was highly acetylated at the γ-OH of the side-chain (48% lignin acetylation), predominantly over G-units; while the lignins from phloem and xylem were barely acetylated and this occurred mainly over S-units. These results are a first time overview of the lignin structure in xylem, phloem (generated by cambium), and in cork (generated by phellogen), in agreement with literature that reports that lignin biosynthesis is flexible and cell specific. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/9b3e9c55-9390-4ec1-86f9-b6b2b1e288e6/download |
| id | ul_be7e1c1f6b4502b30b8a8e5300a79047 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.5/13741 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10400.5/13741 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Lourenço, Ana Rencoret, Jorge Chemetova, Catarina Gominho, Jorge Gutierrez, Ana Rio, José C. del Pereira, Helena |
| publishDate | 2016 |
| publisher.none.fl_str_mv | Danièle Werk (ed.) |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engDanièle Werk (ed.)pt_PTThe composition and structure of lignin in different tissues—phellem (cork), phloem and xylem (wood)—of Quercus suber was studied. Whole cell walls and their respective isolated milled lignins were analyzed by pyrolysis coupled with gas chromatography/mass spectrometry (Py-GC/MS), two-dimensional nuclear magnetic resonance spectroscopy (2D-NMR) and derivatization followed by reductive cleavage (DFRC). Different tissues presented varied p-hydroxyphenyl:guaiacyl:syringyl (H:G:S) lignin compositions. Whereas lignin from cork has a G-rich lignin (H:G:S molar ratio 2:85:13), lignin from phloem presents more S-units (H:G:S molar ratio of 1:58:41) and lignin from xylem is slightly enriched in S-lignin (H:G:S molar ratio 1:45:55). These differences were reflected in the relative abundances of the different interunit linkages. Alkyl-aryl ethers (β–O–4′) were predominant, increasing from 68% in cork, to 71% in phloem and 77% in xylem, as consequence of the enrichment in S-lignin units. Cork lignin was enriched in condensed structures such as phenylcoumarans (β-5′, 20%), dibenzodioxocins (5–5′, 5%), as corresponds to a lignin enriched in G-units. In comparison, lignin from phloem and xylem presented lower levels of condensed linkages. The lignin from cork was highly acetylated at the γ-OH of the side-chain (48% lignin acetylation), predominantly over G-units; while the lignins from phloem and xylem were barely acetylated and this occurred mainly over S-units. These results are a first time overview of the lignin structure in xylem, phloem (generated by cambium), and in cork (generated by phellogen), in agreement with literature that reports that lignin biosynthesis is flexible and cell specific.application/pdfpt_PTLignin composition and structure differs between xylem, phloem and phellem in Quercus suberLourenço, AnaRencoret, JorgeChemetova, CatarinaGominho, JorgeGutierrez, AnaRio, José C. delPereira, HelenaHostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptDOIIsPartOf10.3389/fpls.2016.016122017-06-09T10:25:41Z2016-102016-10-01T00:00:00ZHandlehttp://hdl.handle.net/10400.5/13741http://purl.org/coar/access_right/c_abf2open accesscorkDFRCmilled ligninNMRphloemPy-GC/MSQuercus suberxylem8904135 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/9b3e9c55-9390-4ec1-86f9-b6b2b1e288e6/downloadFrontiers in Plant Science |
| spellingShingle | Lignin composition and structure differs between xylem, phloem and phellem in Quercus suber Lignin composition and structure differs between xylem, phloem and phellem in Quercus suber Lourenço, Ana cork DFRC milled lignin NMR phloem Py-GC/MS Quercus suber xylem Lourenço, Ana cork DFRC milled lignin NMR phloem Py-GC/MS Quercus suber xylem |
| status | SINGLETON |
| subject.fl_str_mv | cork DFRC milled lignin NMR phloem Py-GC/MS Quercus suber xylem |
| title | Lignin composition and structure differs between xylem, phloem and phellem in Quercus suber |
| title_full | Lignin composition and structure differs between xylem, phloem and phellem in Quercus suber |
| title_fullStr | Lignin composition and structure differs between xylem, phloem and phellem in Quercus suber Lignin composition and structure differs between xylem, phloem and phellem in Quercus suber |
| title_full_unstemmed | Lignin composition and structure differs between xylem, phloem and phellem in Quercus suber Lignin composition and structure differs between xylem, phloem and phellem in Quercus suber |
| title_short | Lignin composition and structure differs between xylem, phloem and phellem in Quercus suber |
| title_sort | Lignin composition and structure differs between xylem, phloem and phellem in Quercus suber |
| topic | cork DFRC milled lignin NMR phloem Py-GC/MS Quercus suber xylem |
| topic_facet | cork DFRC milled lignin NMR phloem Py-GC/MS Quercus suber xylem |
| url | http://hdl.handle.net/10400.5/13741 |
| visible | 1 |