Publicação
Effects of Glyphosate-Based Herbicide on Primary Production and Physiological Fitness of the Macroalgae Ulva lactuca
| Resumo: | The use of glyphosate-based herbicides (GBHs) worldwide has increased exponentially over the last two decades increasing the environmental risk to marine and coastal habitats. The present study investigated the effects of GBHs at environmentally relevant concentrations (0, 10, 50, 100, 250, and 500 μg·L−1) on the physiology and biochemistry (photosynthesis, pigment, and lipid composition, antioxidative systems and energy balance) of Ulva lactuca, a cosmopolitan marine macroalgae species. Although GBHs cause deleterious effects such as the inhibition of photosynthetic activity, particularly at 250 μg·L−1, due to the impairment of the electron transport in the chloroplasts, these changes are almost completely reverted at the highest concentration (500 μg·L−1). This could be related to the induction of tolerance mechanisms at a certain threshold or tipping point. While no changes occurred in the energy balance, an increase in the pigment antheraxanthin is observed jointly with an increase in ascorbate peroxidase activity. These mechanisms might have contributed to protecting thylakoids against excess radiation and the increase in reactive oxygen species, associated with stress conditions, as no increase in lipid peroxidation products was observed. Furthermore, changes in the fatty acids profile, usually attributed to the induction of plant stress response mechanisms, demonstrated the high resilience of this macroalgae. Notably, the application of bio-optical tools in ecotoxicology, such as pulse amplitude modulated (PAM) fluorometry and laser-induced fluorescence (LIF), allowed separation of the control samples and those treated by GBHs in different concentrations with a high degree of accuracy, with PAM more accurate in identifying the different treatments. |
|---|---|
| Autores principais: | Cruz De Carvalho, Ricardo |
| Outros Autores: | Feijão, Eduardo; Matos, Ana Rita; Cabrita, Maria Teresa; Utkin, Andrei B.; Novais, Sara C.; Lemos, Marco F. L.; Caçador, Isabel; Marques, João Carlos; Reis-Santos, Patrick; Fonseca, Vanessa F.; Duarte, Bernardo |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1866811285746745344 |
|---|---|
| author | Cruz De Carvalho, Ricardo |
| author2 | Feijão, Eduardo Matos, Ana Rita Cabrita, Maria Teresa Utkin, Andrei B. Novais, Sara C. Lemos, Marco F. L. Caçador, Isabel Marques, João Carlos Reis-Santos, Patrick Fonseca, Vanessa F. Duarte, Bernardo |
| author2_role | author author author author author author author author author author author |
| author_facet | Cruz De Carvalho, Ricardo Feijão, Eduardo Matos, Ana Rita Cabrita, Maria Teresa Utkin, Andrei B. Novais, Sara C. Lemos, Marco F. L. Caçador, Isabel Marques, João Carlos Reis-Santos, Patrick Fonseca, Vanessa F. Duarte, Bernardo |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Cruz De Carvalho, Ricardo\",\"Person.identifier.orcid\":\"0000-0003-4612-9013\"},{\"Person.name\":\"Feijão, Eduardo\"},{\"Person.name\":\"Matos, Ana Rita\"},{\"Person.name\":\"Cabrita, Maria Teresa\"},{\"Person.name\":\"Utkin, Andrei B.\"},{\"Person.name\":\"Novais, Sara C.\"},{\"Person.name\":\"Lemos, Marco F. L.\"},{\"Person.name\":\"Caçador, Isabel\"},{\"Person.name\":\"Marques, João Carlos\"},{\"Person.name\":\"Reis-Santos, Patrick\"},{\"Person.name\":\"Fonseca, Vanessa F.\"},{\"Person.name\":\"Duarte, Bernardo\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Cruz De Carvalho, Ricardo Feijão, Eduardo Matos, Ana Rita Cabrita, Maria Teresa Utkin, Andrei B. Novais, Sara C. Lemos, Marco F. L. Caçador, Isabel Marques, João Carlos Reis-Santos, Patrick Fonseca, Vanessa F. Duarte, Bernardo |
| datacite.date.Accepted.fl_str_mv | 2022-07-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-01-09T19:04:38Z |
| datacite.date.embargoed.fl_str_mv | 2023-01-09T19:04:38Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.titles.title.fl_str_mv | Effects of Glyphosate-Based Herbicide on Primary Production and Physiological Fitness of the Macroalgae Ulva lactuca |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Cruz De Carvalho, Ricardo Feijão, Eduardo Matos, Ana Rita Cabrita, Maria Teresa Utkin, Andrei B. Novais, Sara C. Lemos, Marco F. L. Caçador, Isabel Marques, João Carlos Reis-Santos, Patrick Fonseca, Vanessa F. Duarte, Bernardo |
| dc.date.Accepted.fl_str_mv | 2022-07-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-01-09T19:04:38Z |
| dc.date.embargoed.fl_str_mv | 2023-01-09T19:04:38Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10451/55765 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.title.fl_str_mv | Effects of Glyphosate-Based Herbicide on Primary Production and Physiological Fitness of the Macroalgae Ulva lactuca |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The use of glyphosate-based herbicides (GBHs) worldwide has increased exponentially over the last two decades increasing the environmental risk to marine and coastal habitats. The present study investigated the effects of GBHs at environmentally relevant concentrations (0, 10, 50, 100, 250, and 500 μg·L−1) on the physiology and biochemistry (photosynthesis, pigment, and lipid composition, antioxidative systems and energy balance) of Ulva lactuca, a cosmopolitan marine macroalgae species. Although GBHs cause deleterious effects such as the inhibition of photosynthetic activity, particularly at 250 μg·L−1, due to the impairment of the electron transport in the chloroplasts, these changes are almost completely reverted at the highest concentration (500 μg·L−1). This could be related to the induction of tolerance mechanisms at a certain threshold or tipping point. While no changes occurred in the energy balance, an increase in the pigment antheraxanthin is observed jointly with an increase in ascorbate peroxidase activity. These mechanisms might have contributed to protecting thylakoids against excess radiation and the increase in reactive oxygen species, associated with stress conditions, as no increase in lipid peroxidation products was observed. Furthermore, changes in the fatty acids profile, usually attributed to the induction of plant stress response mechanisms, demonstrated the high resilience of this macroalgae. Notably, the application of bio-optical tools in ecotoxicology, such as pulse amplitude modulated (PAM) fluorometry and laser-induced fluorescence (LIF), allowed separation of the control samples and those treated by GBHs in different concentrations with a high degree of accuracy, with PAM more accurate in identifying the different treatments. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/16e19ed3-baca-4c6e-b1ca-89c1df39db96/download |
| id | ul_c8decd9ea4620f87a88824cfe5ee09d7 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10451/55765 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10451/55765 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Cruz De Carvalho, Ricardo Cruz De Carvalho, Ricardo https://www.ciencia-id.pt/311B-E5EE-6CD7 311B-E5EE-6CD7 http://orcid.org/0000-0003-4612-9013 0000-0003-4612-9013 Feijão, Eduardo Matos, Ana Rita Cabrita, Maria Teresa Utkin, Andrei B. Novais, Sara C. Lemos, Marco F. L. Caçador, Isabel Marques, João Carlos Reis-Santos, Patrick Fonseca, Vanessa F. Duarte, Bernardo |
| publishDate | 2022 |
| publisher.none.fl_str_mv | MDPI |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engMDPIpt_PTThe use of glyphosate-based herbicides (GBHs) worldwide has increased exponentially over the last two decades increasing the environmental risk to marine and coastal habitats. The present study investigated the effects of GBHs at environmentally relevant concentrations (0, 10, 50, 100, 250, and 500 μg·L−1) on the physiology and biochemistry (photosynthesis, pigment, and lipid composition, antioxidative systems and energy balance) of Ulva lactuca, a cosmopolitan marine macroalgae species. Although GBHs cause deleterious effects such as the inhibition of photosynthetic activity, particularly at 250 μg·L−1, due to the impairment of the electron transport in the chloroplasts, these changes are almost completely reverted at the highest concentration (500 μg·L−1). This could be related to the induction of tolerance mechanisms at a certain threshold or tipping point. While no changes occurred in the energy balance, an increase in the pigment antheraxanthin is observed jointly with an increase in ascorbate peroxidase activity. These mechanisms might have contributed to protecting thylakoids against excess radiation and the increase in reactive oxygen species, associated with stress conditions, as no increase in lipid peroxidation products was observed. Furthermore, changes in the fatty acids profile, usually attributed to the induction of plant stress response mechanisms, demonstrated the high resilience of this macroalgae. Notably, the application of bio-optical tools in ecotoxicology, such as pulse amplitude modulated (PAM) fluorometry and laser-induced fluorescence (LIF), allowed separation of the control samples and those treated by GBHs in different concentrations with a high degree of accuracy, with PAM more accurate in identifying the different treatments.application/pdfpt_PTEffects of Glyphosate-Based Herbicide on Primary Production and Physiological Fitness of the Macroalgae Ulva lactucaPersonalCruz De Carvalho, RicardoDSpacehttp://dspace.org/items/5252acf8-8578-4089-bf97-e5736043eb67DSpacehttp://dspace.org/items/5252acf8-8578-4089-bf97-e5736043eb67Cruz de CarvalhoRicardoCiência IDhttps://www.ciencia-id.pt311B-E5EE-6CD7ORCIDhttp://orcid.org0000-0003-4612-9013Researcher IDhttps://www.researcherid.comB-1091-2009Researcher IDhttps://www.researcherid.comB-1091-2009Scopus Author IDhttps://www.scopus.com55365227900Scopus Author IDhttps://www.scopus.com36987421400Feijão, EduardoMatos, Ana RitaCabrita, Maria TeresaUtkin, Andrei B.Novais, Sara C.Lemos, Marco F. L.Caçador, IsabelMarques, João CarlosReis-Santos, PatrickFonseca, Vanessa F.Duarte, BernardoHostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptDOIIsPartOf10.3390/toxics100804302023-01-09T19:04:38Z2022-072022-07-01T00:00:00ZHandlehttp://hdl.handle.net/10451/55765http://purl.org/coar/access_right/c_abf2open access4731822 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2022-07http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/16e19ed3-baca-4c6e-b1ca-89c1df39db96/downloadToxics108430 |
| spellingShingle | Effects of Glyphosate-Based Herbicide on Primary Production and Physiological Fitness of the Macroalgae Ulva lactuca Cruz De Carvalho, Ricardo |
| status | SINGLETON |
| title | Effects of Glyphosate-Based Herbicide on Primary Production and Physiological Fitness of the Macroalgae Ulva lactuca |
| title_full | Effects of Glyphosate-Based Herbicide on Primary Production and Physiological Fitness of the Macroalgae Ulva lactuca |
| title_fullStr | Effects of Glyphosate-Based Herbicide on Primary Production and Physiological Fitness of the Macroalgae Ulva lactuca |
| title_full_unstemmed | Effects of Glyphosate-Based Herbicide on Primary Production and Physiological Fitness of the Macroalgae Ulva lactuca |
| title_short | Effects of Glyphosate-Based Herbicide on Primary Production and Physiological Fitness of the Macroalgae Ulva lactuca |
| title_sort | Effects of Glyphosate-Based Herbicide on Primary Production and Physiological Fitness of the Macroalgae Ulva lactuca |
| url | http://hdl.handle.net/10451/55765 |
| visible | 1 |