Publicação
Acetylcholine and choline effects on erythrocyte nitrite and nitrate levels
| Resumo: | Acetylcholine has been detected in human blood. Acetylcholine receptors and acetylcholinesterase are present in erythrocyte membranes. We tested the acetylcholine and choline effects on nitric oxide metabolites (NOx), namely nitrites and nitrates, and observed if they are dependent on interactions with muscarinic receptors and acetylcholinesterase. Human erythrocyte suspensions were incubated with acetylcholine and choline in the absence or presence of 10 microM atropine or 10 microM velnacrine maleate. The nitrite and nitrate concentrations were determined by the Griess method. Acetylcholine or choline increased NOx control concentrations (P <0.001). The nitrite concentrations decreased in the presence of atropine or velnacrine maleate (P <0.03). The nitrate concentrations only decreased when velnacrine maleate was incubated with acetylcholine or choline (10 microM, P <0.03). These results demonstrated that acetylcholine and choline modulate nitric oxide metabolites on erythrocytes and this effect is mediated by interactions with erythrocyte membrane muscarinic receptors and membrane enzyme acetylcholinesterase. A hypothesis for the signal transduction mechanism has been discussed for acetylcholinesterase and muscarinic receptor (M1) participation. |
|---|---|
| Autores principais: | Carvalho, Filomena Almeida |
| Outros Autores: | Mesquita, Rui; Martins e Silva, João; Saldanha, Carlota |
| Assunto: | Erythrocytes Acetylcholine Choline Nitric oxide metabolites Muscarinic receptor Acetylcholinesterase |
| Ano: | 2004 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1863857325349011456 |
|---|---|
| author | Carvalho, Filomena Almeida |
| author2 | Mesquita, Rui Martins e Silva, João Saldanha, Carlota |
| author2_role | author author author |
| author_facet | Carvalho, Filomena Almeida Mesquita, Rui Martins e Silva, João Saldanha, Carlota |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Carvalho, Filomena Almeida\",\"Person.identifier.orcid\":\"0000-0001-6088-3894\"},{\"Person.name\":\"Mesquita, Rui\"},{\"Person.name\":\"Martins e Silva, João\"},{\"Person.name\":\"Saldanha, Carlota\",\"Person.identifier.orcid\":\"0000-0002-5058-2112\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Carvalho, Filomena Almeida Mesquita, Rui Martins e Silva, João Saldanha, Carlota |
| datacite.date.Accepted.fl_str_mv | 2004-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2020-11-25T14:38:55Z |
| datacite.date.embargoed.fl_str_mv | 2020-11-25T14:38:55Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Erythrocytes Acetylcholine Choline Nitric oxide metabolites Muscarinic receptor Acetylcholinesterase |
| datacite.titles.title.fl_str_mv | Acetylcholine and choline effects on erythrocyte nitrite and nitrate levels |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Carvalho, Filomena Almeida Mesquita, Rui Martins e Silva, João Saldanha, Carlota |
| dc.date.Accepted.fl_str_mv | 2004-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2020-11-25T14:38:55Z |
| dc.date.embargoed.fl_str_mv | 2020-11-25T14:38:55Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10451/45026 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | John Wiley & Sons, Inc. |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Erythrocytes Acetylcholine Choline Nitric oxide metabolites Muscarinic receptor Acetylcholinesterase |
| dc.title.fl_str_mv | Acetylcholine and choline effects on erythrocyte nitrite and nitrate levels |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Acetylcholine has been detected in human blood. Acetylcholine receptors and acetylcholinesterase are present in erythrocyte membranes. We tested the acetylcholine and choline effects on nitric oxide metabolites (NOx), namely nitrites and nitrates, and observed if they are dependent on interactions with muscarinic receptors and acetylcholinesterase. Human erythrocyte suspensions were incubated with acetylcholine and choline in the absence or presence of 10 microM atropine or 10 microM velnacrine maleate. The nitrite and nitrate concentrations were determined by the Griess method. Acetylcholine or choline increased NOx control concentrations (P <0.001). The nitrite concentrations decreased in the presence of atropine or velnacrine maleate (P <0.03). The nitrate concentrations only decreased when velnacrine maleate was incubated with acetylcholine or choline (10 microM, P <0.03). These results demonstrated that acetylcholine and choline modulate nitric oxide metabolites on erythrocytes and this effect is mediated by interactions with erythrocyte membrane muscarinic receptors and membrane enzyme acetylcholinesterase. A hypothesis for the signal transduction mechanism has been discussed for acetylcholinesterase and muscarinic receptor (M1) participation. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/18c9f8c5-4e86-4738-8860-80c891c641ba/download |
| id | ul_c9ea8d61906cf29bc77a331cd1121b21 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10451/45026 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10451/45026 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Carvalho, Filomena Almeida Carvalho, Filomena Almeida https://www.ciencia-id.pt/4615-4AEC-8D91 4615-4AEC-8D91 http://orcid.org/0000-0001-6088-3894 0000-0001-6088-3894 Mesquita, Rui Martins e Silva, João Saldanha, Carlota Saldanha, Carlota http://orcid.org/0000-0002-5058-2112 0000-0002-5058-2112 |
| publishDate | 2004 |
| publisher.none.fl_str_mv | John Wiley & Sons, Inc. |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engJohn Wiley & Sons, Inc.pt_PTAcetylcholine has been detected in human blood. Acetylcholine receptors and acetylcholinesterase are present in erythrocyte membranes. We tested the acetylcholine and choline effects on nitric oxide metabolites (NOx), namely nitrites and nitrates, and observed if they are dependent on interactions with muscarinic receptors and acetylcholinesterase. Human erythrocyte suspensions were incubated with acetylcholine and choline in the absence or presence of 10 microM atropine or 10 microM velnacrine maleate. The nitrite and nitrate concentrations were determined by the Griess method. Acetylcholine or choline increased NOx control concentrations (P <0.001). The nitrite concentrations decreased in the presence of atropine or velnacrine maleate (P <0.03). The nitrate concentrations only decreased when velnacrine maleate was incubated with acetylcholine or choline (10 microM, P <0.03). These results demonstrated that acetylcholine and choline modulate nitric oxide metabolites on erythrocytes and this effect is mediated by interactions with erythrocyte membrane muscarinic receptors and membrane enzyme acetylcholinesterase. A hypothesis for the signal transduction mechanism has been discussed for acetylcholinesterase and muscarinic receptor (M1) participation.application/pdfpt_PTAcetylcholine and choline effects on erythrocyte nitrite and nitrate levelsPersonalCarvalho, Filomena AlmeidaDSpacehttp://dspace.org/items/547cb993-880c-4608-b1e0-493ad469eecbDSpacehttp://dspace.org/items/547cb993-880c-4608-b1e0-493ad469eecbCarvalhoFilomenaCiência IDhttps://www.ciencia-id.pt4615-4AEC-8D91ORCIDhttp://orcid.org0000-0001-6088-3894Researcher IDhttps://www.researcherid.comM-9202-2013Scopus Author IDhttps://www.scopus.com7103069694Mesquita, RuiMartins e Silva, JoãoPersonalSaldanha, CarlotaDSpacehttp://dspace.org/items/ad6de30c-87ca-4613-bb87-488dd5d285d0DSpacehttp://dspace.org/items/ad6de30c-87ca-4613-bb87-488dd5d285d0SaldanhaCarlotaORCIDhttp://orcid.org0000-0002-5058-2112HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptISSNIsPartOf0260-437XDOIIsPartOf10.1002/jat.9932020-11-25T14:38:55Z20042004-01-01T00:00:00ZHandlehttp://hdl.handle.net/10451/45026http://purl.org/coar/access_right/c_16ecrestricted accessErythrocytesAcetylcholineCholineNitric oxide metabolitesMuscarinic receptorAcetylcholinesterase238648 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/18c9f8c5-4e86-4738-8860-80c891c641ba/downloadJournal of Applied Toxicology246419427 |
| spellingShingle | Acetylcholine and choline effects on erythrocyte nitrite and nitrate levels Carvalho, Filomena Almeida Erythrocytes Acetylcholine Choline Nitric oxide metabolites Muscarinic receptor Acetylcholinesterase |
| subject.fl_str_mv | Erythrocytes Acetylcholine Choline Nitric oxide metabolites Muscarinic receptor Acetylcholinesterase |
| title | Acetylcholine and choline effects on erythrocyte nitrite and nitrate levels |
| title_full | Acetylcholine and choline effects on erythrocyte nitrite and nitrate levels |
| title_fullStr | Acetylcholine and choline effects on erythrocyte nitrite and nitrate levels |
| title_full_unstemmed | Acetylcholine and choline effects on erythrocyte nitrite and nitrate levels |
| title_short | Acetylcholine and choline effects on erythrocyte nitrite and nitrate levels |
| title_sort | Acetylcholine and choline effects on erythrocyte nitrite and nitrate levels |
| topic | Erythrocytes Acetylcholine Choline Nitric oxide metabolites Muscarinic receptor Acetylcholinesterase |
| topic_facet | Erythrocytes Acetylcholine Choline Nitric oxide metabolites Muscarinic receptor Acetylcholinesterase |
| url | http://hdl.handle.net/10451/45026 |
| visible | 1 |