Publicação
New insights into the genetic composition and phylogenetic relationship of wolves and dogs in the Iberian Peninsula
| Resumo: | This study investigates the gene pool of Portuguese autochthonous dog breeds and their wild counterpart, the Iberian wolf subspecies (Canis lupus signatus), using standard molecular markers. A combination of paternal and maternal molecular markers was used to investigate the genetic composition, genetic differentiation and genetic relationship of native Portuguese dogs and the Iberian wolf. A total of 196 unrelated dogs, including breed and village dogs from Portugal, and other dogs from Spain and North Africa, and 56 Iberian wolves (wild and captive) were analyzed for nuclear markers, namely Y chromosome SNPs, Y chromosome STR loci, autosomal STR loci, and a mitochondrial fragment of the control region I. Our data reveal new variants for the molecular markers and confirm significant genetic differentiation between Iberian wolf and native domestic dogs from Portugal. Based on our sampling, no signs of recent introgression between the two subspecies were detected. Y chromosome data do not reveal genetic differentiation among the analyzed dog breeds, suggesting they share the same patrilineal origin. Moreover, the genetic distinctiveness of the Iberian wolf from other wolf populations is further confirmed with the description of new mtDNA variants for this endemism. Our research also discloses new molecular markers for wolf and dog subspecies assignment, which might become particularly relevant in the case of forensic or noninvasive genetic studies. The Iberian wolf represents a relic of the once widespread wolf population in Europe and our study reveals that it is a reservoir of unique genetic diversity of the grey wolf, Canis lupus. These results stress the need for conservation plans that will guarantee the sustainability of this threatened top predator in Iberia. |
|---|---|
| Autores principais: | Pires, Ana Elisabete |
| Outros Autores: | Amorim, Isabel R.; Borges, Carla; Simões, Fernanda; Teixeira, Tatiana; Quaresma, Andreia; Petrucci-Fonseca, Francisco; Matos, José |
| Assunto: | Genetic differentiation Genetic diversity Iberian canis Mitochondrial DNA Y chromosome |
| Ano: | 2017 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1866810328983011328 |
|---|---|
| author | Pires, Ana Elisabete |
| author2 | Amorim, Isabel R. Borges, Carla Simões, Fernanda Teixeira, Tatiana Quaresma, Andreia Petrucci-Fonseca, Francisco Matos, José |
| author2_role | author author author author author author author |
| author_facet | Pires, Ana Elisabete Amorim, Isabel R. Borges, Carla Simões, Fernanda Teixeira, Tatiana Quaresma, Andreia Petrucci-Fonseca, Francisco Matos, José |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Pires, Ana Elisabete\"},{\"Person.name\":\"Amorim, Isabel R.\"},{\"Person.name\":\"Borges, Carla\"},{\"Person.name\":\"Simões, Fernanda\"},{\"Person.name\":\"Teixeira, Tatiana\"},{\"Person.name\":\"Quaresma, Andreia\"},{\"Person.name\":\"Petrucci-Fonseca, Francisco\"},{\"Person.name\":\"Matos, José\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Pires, Ana Elisabete Amorim, Isabel R. Borges, Carla Simões, Fernanda Teixeira, Tatiana Quaresma, Andreia Petrucci-Fonseca, Francisco Matos, José |
| datacite.date.Accepted.fl_str_mv | 2017-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-10-30T14:12:51Z |
| datacite.date.embargoed.fl_str_mv | 2018-10-30T14:12:51Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Genetic differentiation Genetic diversity Iberian canis Mitochondrial DNA Y chromosome |
| datacite.titles.title.fl_str_mv | New insights into the genetic composition and phylogenetic relationship of wolves and dogs in the Iberian Peninsula |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Pires, Ana Elisabete Amorim, Isabel R. Borges, Carla Simões, Fernanda Teixeira, Tatiana Quaresma, Andreia Petrucci-Fonseca, Francisco Matos, José |
| dc.date.Accepted.fl_str_mv | 2017-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-10-30T14:12:51Z |
| dc.date.embargoed.fl_str_mv | 2018-10-30T14:12:51Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10451/35230 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Wiley Open Access |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Genetic differentiation Genetic diversity Iberian canis Mitochondrial DNA Y chromosome |
| dc.title.fl_str_mv | New insights into the genetic composition and phylogenetic relationship of wolves and dogs in the Iberian Peninsula |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | This study investigates the gene pool of Portuguese autochthonous dog breeds and their wild counterpart, the Iberian wolf subspecies (Canis lupus signatus), using standard molecular markers. A combination of paternal and maternal molecular markers was used to investigate the genetic composition, genetic differentiation and genetic relationship of native Portuguese dogs and the Iberian wolf. A total of 196 unrelated dogs, including breed and village dogs from Portugal, and other dogs from Spain and North Africa, and 56 Iberian wolves (wild and captive) were analyzed for nuclear markers, namely Y chromosome SNPs, Y chromosome STR loci, autosomal STR loci, and a mitochondrial fragment of the control region I. Our data reveal new variants for the molecular markers and confirm significant genetic differentiation between Iberian wolf and native domestic dogs from Portugal. Based on our sampling, no signs of recent introgression between the two subspecies were detected. Y chromosome data do not reveal genetic differentiation among the analyzed dog breeds, suggesting they share the same patrilineal origin. Moreover, the genetic distinctiveness of the Iberian wolf from other wolf populations is further confirmed with the description of new mtDNA variants for this endemism. Our research also discloses new molecular markers for wolf and dog subspecies assignment, which might become particularly relevant in the case of forensic or noninvasive genetic studies. The Iberian wolf represents a relic of the once widespread wolf population in Europe and our study reveals that it is a reservoir of unique genetic diversity of the grey wolf, Canis lupus. These results stress the need for conservation plans that will guarantee the sustainability of this threatened top predator in Iberia. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/421cb0ce-4f66-42f2-b62e-b16d2cb7fb93/download |
| funding.funder.alternateName_str_mv | FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 3599-PPCDT |
| id | ul_d0d9ea588b4e54e5809cd2fa19ddf16d |
| identifier.url.fl_str_mv | http://hdl.handle.net/10451/35230 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10451/35230 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Pires, Ana Elisabete Amorim, Isabel R. Borges, Carla Simões, Fernanda Teixeira, Tatiana Quaresma, Andreia Petrucci-Fonseca, Francisco Matos, José |
| publishDate | 2017 |
| publisher.none.fl_str_mv | Wiley Open Access |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engWiley Open Accesspt_PTThis study investigates the gene pool of Portuguese autochthonous dog breeds and their wild counterpart, the Iberian wolf subspecies (Canis lupus signatus), using standard molecular markers. A combination of paternal and maternal molecular markers was used to investigate the genetic composition, genetic differentiation and genetic relationship of native Portuguese dogs and the Iberian wolf. A total of 196 unrelated dogs, including breed and village dogs from Portugal, and other dogs from Spain and North Africa, and 56 Iberian wolves (wild and captive) were analyzed for nuclear markers, namely Y chromosome SNPs, Y chromosome STR loci, autosomal STR loci, and a mitochondrial fragment of the control region I. Our data reveal new variants for the molecular markers and confirm significant genetic differentiation between Iberian wolf and native domestic dogs from Portugal. Based on our sampling, no signs of recent introgression between the two subspecies were detected. Y chromosome data do not reveal genetic differentiation among the analyzed dog breeds, suggesting they share the same patrilineal origin. Moreover, the genetic distinctiveness of the Iberian wolf from other wolf populations is further confirmed with the description of new mtDNA variants for this endemism. Our research also discloses new molecular markers for wolf and dog subspecies assignment, which might become particularly relevant in the case of forensic or noninvasive genetic studies. The Iberian wolf represents a relic of the once widespread wolf population in Europe and our study reveals that it is a reservoir of unique genetic diversity of the grey wolf, Canis lupus. These results stress the need for conservation plans that will guarantee the sustainability of this threatened top predator in Iberia.application/pdfpt_PTNew insights into the genetic composition and phylogenetic relationship of wolves and dogs in the Iberian PeninsulaPires, Ana ElisabeteAmorim, Isabel R.Borges, CarlaSimões, FernandaTeixeira, TatianaQuaresma, AndreiaPetrucci-Fonseca, FranciscoMatos, JoséHostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptISSNIsPartOf2045-7758DOIIsPartOf10.1002/ece3.29492018-10-30T14:12:51Z20172017-01-01T00:00:00ZHandlehttp://hdl.handle.net/10451/35230http://purl.org/coar/access_right/c_abf2open accessGenetic differentiationGenetic diversityIberian canisMitochondrial DNAY chromosome818995 bytesFundação para a Ciência e a TecnologiaTracing back animal domestication in Iberia: Clues from zooarchaeology and DNA analysis3599-PPCDTCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaBiodiv- WhoCares – Communicating the value of unique biodiversity: targeting non-traditional audiences in the Azores.Crossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/421cb0ce-4f66-42f2-b62e-b16d2cb7fb93/downloadEcology and Evolution7(12)44044418 |
| spellingShingle | New insights into the genetic composition and phylogenetic relationship of wolves and dogs in the Iberian Peninsula Pires, Ana Elisabete Genetic differentiation Genetic diversity Iberian canis Mitochondrial DNA Y chromosome |
| status | SINGLETON |
| subject.fl_str_mv | Genetic differentiation Genetic diversity Iberian canis Mitochondrial DNA Y chromosome |
| title | New insights into the genetic composition and phylogenetic relationship of wolves and dogs in the Iberian Peninsula |
| title_full | New insights into the genetic composition and phylogenetic relationship of wolves and dogs in the Iberian Peninsula |
| title_fullStr | New insights into the genetic composition and phylogenetic relationship of wolves and dogs in the Iberian Peninsula |
| title_full_unstemmed | New insights into the genetic composition and phylogenetic relationship of wolves and dogs in the Iberian Peninsula |
| title_short | New insights into the genetic composition and phylogenetic relationship of wolves and dogs in the Iberian Peninsula |
| title_sort | New insights into the genetic composition and phylogenetic relationship of wolves and dogs in the Iberian Peninsula |
| topic | Genetic differentiation Genetic diversity Iberian canis Mitochondrial DNA Y chromosome |
| topic_facet | Genetic differentiation Genetic diversity Iberian canis Mitochondrial DNA Y chromosome |
| url | http://hdl.handle.net/10451/35230 |
| visible | 1 |