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Tight controlled expression and secretion of Lactobacillus brevis SlpA in Lactococcus lactis

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Resumo:Prokaryotes commonly present outer cell wall structures composed of a crystalline array of proteinaceous subunits, known as surface layers (S-layers). The ORF encoding the S-layer protein (SlpA) of Lactobacillus brevis was cloned into Lactococcus lactis under the transcriptional control of the xyloseinducible expression system (XIES). SlpA was secreted into the extracellular medium, as determined by immunoblotting, and assays on the kinetics of SlpA production revealed that repression of the system with glucose did not require the depletion of xylose from the medium that allows transitory ORF expression. The successful use of XIES to express S-layer proteins in the versatile and generally recognized as safe species L. lactis opens new possibilities for an efficient production and isolation of SlpA S-layer protein for its various applications in biotechnology and importantly as an antigen-carrying vehicle.
Autores principais:Hollmann, Axel
Outros Autores:Saviello, Mariano; Delfederico, Lucrecia; Saraiva, Tessália Diniz Luerce; Barh, Debmalya; Jain, Neha; Tiwari, Sandeep; Chandra, Sudha; Gupta, Krishnakant; Zambare, Vasudeo; Kumar, Anil; Christopher, Lew; Misra, Amarendra Narayan; Kumavath, Ranjith N.; Azevedo, Vasco; Semorile, Liliana; Miyoshi, Anderson
Assunto:Inducible heterologous expression Lactobacillus brevis Lactococcus lactis S-layer protein
Ano:2012
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso restrito
Instituição associada:Universidade de Lisboa
Idioma:inglês
Origem:Repositório da Universidade de Lisboa

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