Publicação
Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcoding
| Resumo: | 1. DNA metabarcoding is widely used to characterize the diet of species, and it becomes very relevant for biodiversity conservation, allowing the understanding of trophic chains and the impact of invasive species. The need for cost-effective biodiversity monitoring methods fostered advances in this technique. One question that arises is which sample type provides a better diet representation. 2. Therefore, with this study, we intended to evaluate if there were differences in diet estimates according to the section of the gastrointestinal tract analysed and which section(s) provided the best diet representation. Additionally, we intended to infer the ecological/economic impacts of an invader as a model of the potential effects in an originally mammal-free ecosystem. 3. We examined the gut contents of the house mouse Mus musculus introduced to Cabo Verde, considering three sections: stomach, small intestine, and large intestine. We applied a DNA-metabarcoding approach using two genetic markers, one specific for plants and another for invertebrates. 4. We showed that this invader consumed 131 taxa (73 plants and 58 invertebrates). We obtained significant differences in the composition of two of the three sections, with a higher incidence of invertebrates in the stomach and plants in the intestines. This may be due to stomach inhibitors acting on plants and/or to faster absorption of soft-body invertebrates compared to the plant fibers in the intestines. We verified that the impact of this invader in the ecosystem is predominantly negative, as at least 50% of the ingested items were native, endemic, or economically important taxa, and only 19% of the diet items were exotics 5. Overall, results showed the need to analyse only two gastrointestinal tract sections to obtain robust diet data, increasing the cost-effectiveness of the method. Furthermore, by uncovering the native taxa most frequently preyed on by mice, this DNA-metabarcoding approach allowed us to evaluate efficiently which are at the highest risk |
|---|---|
| Autores principais: | Pinho, C.J. |
| Outros Autores: | Lopes, E.P.; Paupério, J.; Gomes, I.; Romeiras, Maria M.; Vasconcelos, R. |
| Assunto: | Cabo Verde Islands diet gastrointestinal tract house mouse invasive species invertebrates next-generation sequencing plants |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1866811012497276928 |
|---|---|
| author | Pinho, C.J. |
| author2 | Lopes, E.P. Paupério, J. Gomes, I. Romeiras, Maria M. Vasconcelos, R. |
| author2_role | author author author author author |
| author_facet | Pinho, C.J. Lopes, E.P. Paupério, J. Gomes, I. Romeiras, Maria M. Vasconcelos, R. |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Pinho, C.J.\"},{\"Person.name\":\"Lopes, E.P.\"},{\"Person.name\":\"Paupério, J.\"},{\"Person.name\":\"Gomes, I.\"},{\"Person.name\":\"Romeiras, Maria M.\",\"Person.identifier.orcid\":\"0000-0002-9373-6302\"},{\"Person.name\":\"Vasconcelos, R.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Pinho, C.J. Lopes, E.P. Paupério, J. Gomes, I. Romeiras, Maria M. Vasconcelos, R. |
| datacite.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2022-04-18T10:59:05Z |
| datacite.date.embargoed.fl_str_mv | 2022-04-18T10:59:05Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Cabo Verde Islands diet gastrointestinal tract house mouse invasive species invertebrates next-generation sequencing plants |
| datacite.titles.title.fl_str_mv | Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcoding |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Pinho, C.J. Lopes, E.P. Paupério, J. Gomes, I. Romeiras, Maria M. Vasconcelos, R. |
| dc.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2022-04-18T10:59:05Z |
| dc.date.embargoed.fl_str_mv | 2022-04-18T10:59:05Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.5/24094 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Wiley |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Cabo Verde Islands diet gastrointestinal tract house mouse invasive species invertebrates next-generation sequencing plants |
| dc.title.fl_str_mv | Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcoding |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | 1. DNA metabarcoding is widely used to characterize the diet of species, and it becomes very relevant for biodiversity conservation, allowing the understanding of trophic chains and the impact of invasive species. The need for cost-effective biodiversity monitoring methods fostered advances in this technique. One question that arises is which sample type provides a better diet representation. 2. Therefore, with this study, we intended to evaluate if there were differences in diet estimates according to the section of the gastrointestinal tract analysed and which section(s) provided the best diet representation. Additionally, we intended to infer the ecological/economic impacts of an invader as a model of the potential effects in an originally mammal-free ecosystem. 3. We examined the gut contents of the house mouse Mus musculus introduced to Cabo Verde, considering three sections: stomach, small intestine, and large intestine. We applied a DNA-metabarcoding approach using two genetic markers, one specific for plants and another for invertebrates. 4. We showed that this invader consumed 131 taxa (73 plants and 58 invertebrates). We obtained significant differences in the composition of two of the three sections, with a higher incidence of invertebrates in the stomach and plants in the intestines. This may be due to stomach inhibitors acting on plants and/or to faster absorption of soft-body invertebrates compared to the plant fibers in the intestines. We verified that the impact of this invader in the ecosystem is predominantly negative, as at least 50% of the ingested items were native, endemic, or economically important taxa, and only 19% of the diet items were exotics 5. Overall, results showed the need to analyse only two gastrointestinal tract sections to obtain robust diet data, increasing the cost-effectiveness of the method. Furthermore, by uncovering the native taxa most frequently preyed on by mice, this DNA-metabarcoding approach allowed us to evaluate efficiently which are at the highest risk |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/f8ec6f84-105b-4139-8e4a-5b433bafa3cb/download |
| funding.funder.alternateName_str_mv | FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 6817 - DCRRNI ID |
| id | ul_d7fc62e8b74cd338dedfbd908affc292 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.5/24094 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10400.5/24094 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Pinho, C.J. Lopes, E.P. Paupério, J. Gomes, I. Romeiras, Maria M. Romeiras, Maria M. https://www.ciencia-id.pt/6A1C-085E-9038 6A1C-085E-9038 http://orcid.org/0000-0002-9373-6302 0000-0002-9373-6302 Vasconcelos, R. |
| publishDate | 2022 |
| publisher.none.fl_str_mv | Wiley |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engWileypt_PT1. DNA metabarcoding is widely used to characterize the diet of species, and it becomes very relevant for biodiversity conservation, allowing the understanding of trophic chains and the impact of invasive species. The need for cost-effective biodiversity monitoring methods fostered advances in this technique. One question that arises is which sample type provides a better diet representation. 2. Therefore, with this study, we intended to evaluate if there were differences in diet estimates according to the section of the gastrointestinal tract analysed and which section(s) provided the best diet representation. Additionally, we intended to infer the ecological/economic impacts of an invader as a model of the potential effects in an originally mammal-free ecosystem. 3. We examined the gut contents of the house mouse Mus musculus introduced to Cabo Verde, considering three sections: stomach, small intestine, and large intestine. We applied a DNA-metabarcoding approach using two genetic markers, one specific for plants and another for invertebrates. 4. We showed that this invader consumed 131 taxa (73 plants and 58 invertebrates). We obtained significant differences in the composition of two of the three sections, with a higher incidence of invertebrates in the stomach and plants in the intestines. This may be due to stomach inhibitors acting on plants and/or to faster absorption of soft-body invertebrates compared to the plant fibers in the intestines. We verified that the impact of this invader in the ecosystem is predominantly negative, as at least 50% of the ingested items were native, endemic, or economically important taxa, and only 19% of the diet items were exotics 5. Overall, results showed the need to analyse only two gastrointestinal tract sections to obtain robust diet data, increasing the cost-effectiveness of the method. Furthermore, by uncovering the native taxa most frequently preyed on by mice, this DNA-metabarcoding approach allowed us to evaluate efficiently which are at the highest riskapplication/pdfpt_PTTrust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcodingPinho, C.J.Lopes, E.P.Paupério, J.Gomes, I.PersonalRomeiras, Maria M.DSpacehttp://dspace.org/items/a66d151c-8bfa-4f32-ba8e-b20f9870ba24DSpacehttp://dspace.org/items/a66d151c-8bfa-4f32-ba8e-b20f9870ba24RomeirasMariaCiência IDhttps://www.ciencia-id.pt6A1C-085E-9038ORCIDhttp://orcid.org0000-0002-9373-6302Researcher IDhttps://www.researcherid.comJ-4552-2013Scopus Author IDhttps://www.scopus.com15842082800Vasconcelos, R.HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptDOIIsPartOfhttps://doi.org/10.1002/ece3.86382022-04-18T10:59:05Z20222022-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.5/24094http://purl.org/coar/access_right/c_abf2open accessCabo Verde Islandsdietgastrointestinal tracthouse mouseinvasive speciesinvertebratesnext-generation sequencingplants2436578 bytesFundação para a Ciência e a TecnologiaLinking Landscape, Environment, Agriculture and Food6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal article2022http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/f8ec6f84-105b-4139-8e4a-5b433bafa3cb/downloadEcology and Evolution |
| spellingShingle | Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcoding Pinho, C.J. Cabo Verde Islands diet gastrointestinal tract house mouse invasive species invertebrates next-generation sequencing plants |
| status | SINGLETON |
| subject.fl_str_mv | Cabo Verde Islands diet gastrointestinal tract house mouse invasive species invertebrates next-generation sequencing plants |
| title | Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcoding |
| title_full | Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcoding |
| title_fullStr | Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcoding |
| title_full_unstemmed | Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcoding |
| title_short | Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcoding |
| title_sort | Trust your guts? The effect of gut section on diet composition and impact of Mus musculus on islands using metabarcoding |
| topic | Cabo Verde Islands diet gastrointestinal tract house mouse invasive species invertebrates next-generation sequencing plants |
| topic_facet | Cabo Verde Islands diet gastrointestinal tract house mouse invasive species invertebrates next-generation sequencing plants |
| url | http://hdl.handle.net/10400.5/24094 |
| visible | 1 |