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Qualidade de amendoins consumidos em Portugal : flora fúngica e pesquisa de micotoxinas

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Resumo:Mycotoxins are fungal secondary metabolites that may contaminate food and feed. Chronic ou acute exposure to mycotoxins may induce illness in humans and animals, mainly by its hepatotoxic, nephrotoxic and carcinogenic effects. The mycological and mycotoxicological contamination were analysed in roasted peanuts, available in the portuguese market. Twelve samples, mostly pre-packed, were analysed. Mycological content, determined by dilution followed by plating in Rose Bengal Chloramphenicol Agar medium, revealed 11 samples contaminated by molds, with average mycological content of 3.7 x 103 CFU/g. Most frequently isolated molds were Penicillium chrysogenum, Cladosporium spp. and Alternaria spp. Fungi generally associated with mycotoxin production were also isolated, as Aspergillus flavus and Aspergillus niger. Samples were purified with immunoaffinity columns, and mycotoxin determination, by high performance liquid chromatography with ultra-violet detection, carried on aflatoxin B1 and ochratoxin A. Detection frequency for aflatoxin was 50%, with content varying between 0.199 and 1.506 µg/Kg, below the European Union maximum authorized limit. Only one sample has shown detectable ochratoxin A concentration, 1.072 µg/Kg
Autores principais:Silvério, André Ferreira Alves, 1981-
Assunto:Micotoxinas Aflatoxina B1 Ocratoxina A Fungos Cromatografia líquida de alta resolução Portugal Teses de mestrado - 2009
Ano:2009
País:Portugal
Tipo de documento:dissertação de mestrado
Tipo de acesso:acesso restrito
Instituição associada:Universidade de Lisboa
Idioma:português
Origem:Repositório da Universidade de Lisboa

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