Publicação
Metallo-beta-lactamase VIM-2 in clinical isolates of Pseudomonas aeruginosa from Portugal
| Resumo: | Resistance to carbapenems is emerging, and it is a great problem to therapeutics. Three isolates of Pseudomonas aeruginosa from a Portuguese hospital identified in urine and sputum, in 1995, presented a high-level resistance to imipenem (32 mg/L). Afterward, one isolate of P. aeruginosa recovered from urine of an ambulatory patient in 1998 showed high resistance to imipenem. and meropenem. The resistance to carbapenems in these strains was associated with the production of a class B beta-lactamase, as was demonstrated by imipenem hydrolysis and inhibition by EDTA. Using primers described for bla(IMP) and bla(VIM) the amplification of the latter was observed in all isolates and a VIM-2 metallo-enzyme was identified. The pulsed-field gel electrophoresis (PFGE) patterns of these isolates were indistinguishable, suggesting dissemination to the community of this VIM-2 producer. |
|---|---|
| Autores principais: | Cardoso, O |
| Outros Autores: | Leitao, R; Figueiredo, A; Sousa, JC; Duarte, A; Peixe, LV |
| Assunto: | Infectious Diseases Microbiology Pharmacology & Pharmacy |
| Ano: | 2002 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso a metadados |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1865920843678220288 |
|---|---|
| author | Cardoso, O |
| author2 | Leitao, R Figueiredo, A Sousa, JC Duarte, A Peixe, LV |
| author2_role | author author author author author |
| author_facet | Cardoso, O Cardoso, O Leitao, R Figueiredo, A Sousa, JC Duarte, A Peixe, LV Leitao, R Figueiredo, A Sousa, JC Duarte, A Peixe, LV |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Cardoso, O\"},{\"Person.name\":\"Leitao, R\"},{\"Person.name\":\"Figueiredo, A\"},{\"Person.name\":\"Sousa, JC\"},{\"Person.name\":\"Duarte, A\"},{\"Person.name\":\"Peixe, LV\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Cardoso, O Leitao, R Figueiredo, A Sousa, JC Duarte, A Peixe, LV |
| datacite.date.Accepted.fl_str_mv | 2002-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2015-12-30T10:17:58Z |
| datacite.date.embargoed.fl_str_mv | 2015-12-30T10:17:58Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_14cb |
| datacite.subjects.subject.fl_str_mv | Infectious Diseases Microbiology Pharmacology & Pharmacy |
| datacite.titles.title.fl_str_mv | Metallo-beta-lactamase VIM-2 in clinical isolates of Pseudomonas aeruginosa from Portugal |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Cardoso, O Leitao, R Figueiredo, A Sousa, JC Duarte, A Peixe, LV |
| dc.date.Accepted.fl_str_mv | 2002-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2015-12-30T10:17:58Z |
| dc.date.embargoed.fl_str_mv | 2015-12-30T10:17:58Z |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10451/21340 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MARY ANN LIEBERT INC PUBL |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_14cb |
| dc.subject.none.fl_str_mv | Infectious Diseases Microbiology Pharmacology & Pharmacy |
| dc.title.fl_str_mv | Metallo-beta-lactamase VIM-2 in clinical isolates of Pseudomonas aeruginosa from Portugal |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Resistance to carbapenems is emerging, and it is a great problem to therapeutics. Three isolates of Pseudomonas aeruginosa from a Portuguese hospital identified in urine and sputum, in 1995, presented a high-level resistance to imipenem (32 mg/L). Afterward, one isolate of P. aeruginosa recovered from urine of an ambulatory patient in 1998 showed high resistance to imipenem. and meropenem. The resistance to carbapenems in these strains was associated with the production of a class B beta-lactamase, as was demonstrated by imipenem hydrolysis and inhibition by EDTA. Using primers described for bla(IMP) and bla(VIM) the amplification of the latter was observed in all isolates and a VIM-2 metallo-enzyme was identified. The pulsed-field gel electrophoresis (PFGE) patterns of these isolates were indistinguishable, suggesting dissemination to the community of this VIM-2 producer. |
| dirty | 0 |
| eu_rights_str_mv | metadataOnlyAccess |
| format | article |
| id | ul_dfef6eeec09ef4e2378cbc59dfa29023 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10451/21340 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10451/21340 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Cardoso, O Leitao, R Figueiredo, A Sousa, JC Duarte, A Peixe, LV |
| publishDate | 2002 |
| publisher.none.fl_str_mv | MARY ANN LIEBERT INC PUBL |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engMARY ANN LIEBERT INC PUBLResistance to carbapenems is emerging, and it is a great problem to therapeutics. Three isolates of Pseudomonas aeruginosa from a Portuguese hospital identified in urine and sputum, in 1995, presented a high-level resistance to imipenem (32 mg/L). Afterward, one isolate of P. aeruginosa recovered from urine of an ambulatory patient in 1998 showed high resistance to imipenem. and meropenem. The resistance to carbapenems in these strains was associated with the production of a class B beta-lactamase, as was demonstrated by imipenem hydrolysis and inhibition by EDTA. Using primers described for bla(IMP) and bla(VIM) the amplification of the latter was observed in all isolates and a VIM-2 metallo-enzyme was identified. The pulsed-field gel electrophoresis (PFGE) patterns of these isolates were indistinguishable, suggesting dissemination to the community of this VIM-2 producer.Metallo-beta-lactamase VIM-2 in clinical isolates of Pseudomonas aeruginosa from PortugalCardoso, OLeitao, RFigueiredo, ASousa, JCDuarte, APeixe, LVHostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptISSNIsPartOf1076-62942015-12-30T10:17:58Z20022002-01-01T00:00:00ZHandlehttp://hdl.handle.net/10451/21340http://purl.org/coar/access_right/c_14cbmetadata only accessInfectious DiseasesMicrobiologyPharmacology & Pharmacyliteraturehttp://purl.org/coar/resource_type/c_6501journal articleMICROBIAL DRUG RESISTANCE-MECHANISMS EPIDEMIOLOGY AND DISEASEVol. 89397 |
| spellingShingle | Metallo-beta-lactamase VIM-2 in clinical isolates of Pseudomonas aeruginosa from Portugal Metallo-beta-lactamase VIM-2 in clinical isolates of Pseudomonas aeruginosa from Portugal Cardoso, O Infectious Diseases Microbiology Pharmacology & Pharmacy Cardoso, O Infectious Diseases Microbiology Pharmacology & Pharmacy |
| status | SINGLETON |
| subject.fl_str_mv | Infectious Diseases Microbiology Pharmacology & Pharmacy |
| title | Metallo-beta-lactamase VIM-2 in clinical isolates of Pseudomonas aeruginosa from Portugal |
| title_full | Metallo-beta-lactamase VIM-2 in clinical isolates of Pseudomonas aeruginosa from Portugal |
| title_fullStr | Metallo-beta-lactamase VIM-2 in clinical isolates of Pseudomonas aeruginosa from Portugal Metallo-beta-lactamase VIM-2 in clinical isolates of Pseudomonas aeruginosa from Portugal |
| title_full_unstemmed | Metallo-beta-lactamase VIM-2 in clinical isolates of Pseudomonas aeruginosa from Portugal Metallo-beta-lactamase VIM-2 in clinical isolates of Pseudomonas aeruginosa from Portugal |
| title_short | Metallo-beta-lactamase VIM-2 in clinical isolates of Pseudomonas aeruginosa from Portugal |
| title_sort | Metallo-beta-lactamase VIM-2 in clinical isolates of Pseudomonas aeruginosa from Portugal |
| topic | Infectious Diseases Microbiology Pharmacology & Pharmacy |
| topic_facet | Infectious Diseases Microbiology Pharmacology & Pharmacy |
| url | http://hdl.handle.net/10451/21340 |
| visible | 1 |