Publicação
Polyclonal infection as a new scenario in Mycobacterium caprae epidemiology
| Resumo: | Portugal is one of the European Union countries with an ongoing eradication program for bovine tuberculosis (TB), which does not include systematic goat testing. However, surveillance in small ruminants is increasingly important, since goat and sheep can harbour Mycobacterium caprae and be an infection source to cattle with impact in the success of bovine TB control. Furthermore, the information regarding the epidemiology and biology of M. caprae is quite limited comparing to the cognate bovine-adapted ecotype, M. bovis. In this work, we applied spoligotyping and MIRU-VNTR (Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats) to M. caprae obtained between 2003 and 2014 from 55 animal hosts, including goat (n = 29), cattle (n = 21), sheep (n = 1) and wild boar (n = 4) from Portugal. The molecular analysis revealed a unique spoligotyping pattern (SB0157) and 24 MIRU types. Genotyping of serial M. caprae from herds with recurrent outbreaks enabled further discrimination of epidemiologically related isolates, supporting a clonal structure in Portugal and denoting the emergence of clonal diversity at the herd level, more apparent for MIRU4. Results suggest a founder effect and adaptive genotypic divergence, paving the way for sympatric speciation. Double allele findings at MIRU4 in over 20 % of infected animals indicates that co-infection and in vivo microevolution may be frequent in the goat-adapted ecotype. While polyclonal infection appears common in M. caprae epidemiology, the functional significance of subtle genotypic variations remains to be disclosed, namely at the interface with the host, to expand knowledge on the epidemiology and biology of this neglected ecotype. |
|---|---|
| Autores principais: | Reis, Ana C. |
| Outros Autores: | Albuquerque, Teresa; Botelho, Ana; Cunha, Mónica V. |
| Assunto: | Mycobacterium caprae Animal tuberculosis Spoligotyping VNTR Clonal diversity Polyclonal infection |
| Ano: | 2020 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1866811231001640960 |
|---|---|
| author | Reis, Ana C. |
| author2 | Albuquerque, Teresa Botelho, Ana Cunha, Mónica V. |
| author2_role | author author author |
| author_facet | Reis, Ana C. Albuquerque, Teresa Botelho, Ana Cunha, Mónica V. |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Reis, Ana C.\"},{\"Person.name\":\"Albuquerque, Teresa\"},{\"Person.name\":\"Botelho, Ana\"},{\"Person.name\":\"Cunha, Mónica V.\",\"Person.identifier.orcid\":\"0000-0003-0401-0276\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Reis, Ana C. Albuquerque, Teresa Botelho, Ana Cunha, Mónica V. |
| datacite.date.Accepted.fl_str_mv | 2020-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2021-01-29T20:16:43Z |
| datacite.date.embargoed.fl_str_mv | 2021-01-29T20:16:43Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Mycobacterium caprae Animal tuberculosis Spoligotyping VNTR Clonal diversity Polyclonal infection |
| datacite.titles.title.fl_str_mv | Polyclonal infection as a new scenario in Mycobacterium caprae epidemiology |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Reis, Ana C. Albuquerque, Teresa Botelho, Ana Cunha, Mónica V. |
| dc.date.Accepted.fl_str_mv | 2020-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2021-01-29T20:16:43Z |
| dc.date.embargoed.fl_str_mv | 2021-01-29T20:16:43Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10451/46055 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Mycobacterium caprae Animal tuberculosis Spoligotyping VNTR Clonal diversity Polyclonal infection |
| dc.title.fl_str_mv | Polyclonal infection as a new scenario in Mycobacterium caprae epidemiology |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Portugal is one of the European Union countries with an ongoing eradication program for bovine tuberculosis (TB), which does not include systematic goat testing. However, surveillance in small ruminants is increasingly important, since goat and sheep can harbour Mycobacterium caprae and be an infection source to cattle with impact in the success of bovine TB control. Furthermore, the information regarding the epidemiology and biology of M. caprae is quite limited comparing to the cognate bovine-adapted ecotype, M. bovis. In this work, we applied spoligotyping and MIRU-VNTR (Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats) to M. caprae obtained between 2003 and 2014 from 55 animal hosts, including goat (n = 29), cattle (n = 21), sheep (n = 1) and wild boar (n = 4) from Portugal. The molecular analysis revealed a unique spoligotyping pattern (SB0157) and 24 MIRU types. Genotyping of serial M. caprae from herds with recurrent outbreaks enabled further discrimination of epidemiologically related isolates, supporting a clonal structure in Portugal and denoting the emergence of clonal diversity at the herd level, more apparent for MIRU4. Results suggest a founder effect and adaptive genotypic divergence, paving the way for sympatric speciation. Double allele findings at MIRU4 in over 20 % of infected animals indicates that co-infection and in vivo microevolution may be frequent in the goat-adapted ecotype. While polyclonal infection appears common in M. caprae epidemiology, the functional significance of subtle genotypic variations remains to be disclosed, namely at the interface with the host, to expand knowledge on the epidemiology and biology of this neglected ecotype. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/919cdac6-6b8b-41ff-98b9-855e819479a7/download |
| funding.funder.alternateName_str_mv | FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 6817 - DCRRNI ID FARH |
| id | ul_e02be8193ce9b9ff44bd96dfe4eab20c |
| identifier.url.fl_str_mv | http://hdl.handle.net/10451/46055 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10451/46055 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Reis, Ana C. Albuquerque, Teresa Botelho, Ana Cunha, Mónica V. Cunha, Mónica V. https://www.ciencia-id.pt/5616-E715-2F31 5616-E715-2F31 http://orcid.org/0000-0003-0401-0276 0000-0003-0401-0276 |
| publishDate | 2020 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engElsevierpt_PTPortugal is one of the European Union countries with an ongoing eradication program for bovine tuberculosis (TB), which does not include systematic goat testing. However, surveillance in small ruminants is increasingly important, since goat and sheep can harbour Mycobacterium caprae and be an infection source to cattle with impact in the success of bovine TB control. Furthermore, the information regarding the epidemiology and biology of M. caprae is quite limited comparing to the cognate bovine-adapted ecotype, M. bovis. In this work, we applied spoligotyping and MIRU-VNTR (Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats) to M. caprae obtained between 2003 and 2014 from 55 animal hosts, including goat (n = 29), cattle (n = 21), sheep (n = 1) and wild boar (n = 4) from Portugal. The molecular analysis revealed a unique spoligotyping pattern (SB0157) and 24 MIRU types. Genotyping of serial M. caprae from herds with recurrent outbreaks enabled further discrimination of epidemiologically related isolates, supporting a clonal structure in Portugal and denoting the emergence of clonal diversity at the herd level, more apparent for MIRU4. Results suggest a founder effect and adaptive genotypic divergence, paving the way for sympatric speciation. Double allele findings at MIRU4 in over 20 % of infected animals indicates that co-infection and in vivo microevolution may be frequent in the goat-adapted ecotype. While polyclonal infection appears common in M. caprae epidemiology, the functional significance of subtle genotypic variations remains to be disclosed, namely at the interface with the host, to expand knowledge on the epidemiology and biology of this neglected ecotype.application/pdfpt_PTPolyclonal infection as a new scenario in Mycobacterium caprae epidemiologyReis, Ana C.Albuquerque, TeresaBotelho, AnaPersonalCunha, Mónica V.DSpacehttp://dspace.org/items/a1b365bb-7421-4bf5-9cde-62b74c170e69DSpacehttp://dspace.org/items/a1b365bb-7421-4bf5-9cde-62b74c170e69CunhaMonica V.Ciência IDhttps://www.ciencia-id.pt5616-E715-2F31ORCIDhttp://orcid.org0000-0003-0401-0276Researcher IDhttps://www.researcherid.comD-5544-2011Scopus Author IDhttps://www.scopus.com7102711216HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptDOIIsPartOf10.1016/j.vetmic.2019.1085332021-01-29T20:16:43Z2020-012020-01-01T00:00:00ZHandlehttp://hdl.handle.net/10451/46055http://purl.org/coar/access_right/c_abf2open accessMycobacterium capraeAnimal tuberculosisSpoligotypingVNTRClonal diversityPolyclonal infection1118420 bytesFundação para a Ciência e a TecnologiaCentre for Ecology, Evolution and Environmental Changes6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaSystems epidemiology to devise new interventions for multi-host tuberculosisFARHCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal article2020-01http://creativecommons.org/licenses/by-nc-nd/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/919cdac6-6b8b-41ff-98b9-855e819479a7/downloadVeterinary Microbiology240108533 |
| spellingShingle | Polyclonal infection as a new scenario in Mycobacterium caprae epidemiology Reis, Ana C. Mycobacterium caprae Animal tuberculosis Spoligotyping VNTR Clonal diversity Polyclonal infection |
| status | SINGLETON |
| subject.fl_str_mv | Mycobacterium caprae Animal tuberculosis Spoligotyping VNTR Clonal diversity Polyclonal infection |
| title | Polyclonal infection as a new scenario in Mycobacterium caprae epidemiology |
| title_full | Polyclonal infection as a new scenario in Mycobacterium caprae epidemiology |
| title_fullStr | Polyclonal infection as a new scenario in Mycobacterium caprae epidemiology |
| title_full_unstemmed | Polyclonal infection as a new scenario in Mycobacterium caprae epidemiology |
| title_short | Polyclonal infection as a new scenario in Mycobacterium caprae epidemiology |
| title_sort | Polyclonal infection as a new scenario in Mycobacterium caprae epidemiology |
| topic | Mycobacterium caprae Animal tuberculosis Spoligotyping VNTR Clonal diversity Polyclonal infection |
| topic_facet | Mycobacterium caprae Animal tuberculosis Spoligotyping VNTR Clonal diversity Polyclonal infection |
| url | http://hdl.handle.net/10451/46055 |
| visible | 1 |