Publicação
Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans
| Resumo: | This study aimed to characterize the fecal colonization and sharing of Klebsiella pneumoniae strains between companion animals and humans living in close contact. Fecal samples were collected from 50 healthy participants (24 humans, 18 dogs, and 8 cats) belonging to 18 households. Samples were plated onto MacConkey agar (MCK) plates with and without cefotaxime or meropenem supplementation. Up to five K. pneumoniae colonies per participant were compared by pulsed-field gel electrophoresis (PFGE) after XbaI restriction. K. pneumoniae strains with unique pulse types from each participant were characterized for antimicrobial susceptibility, virulence genes, and multilocus sequence type (MLST). Fecal K. pneumoniae pulse types were compared to those of clinical K. pneumoniae strains from animal and human patients with urinary tract infections (n = 104). K. pneumoniae colonization was detected in nonsupplemented MCK in around 38% of dogs (n = 7) and humans (n = 9). K. pneumoniae strains isolated from dogs belonged to sequence type 17 (ST17), ST188, ST252, ST281, ST423, ST1093, ST1241, ST3398, and ST3399. None of the K. pneumoniae strains were multidrug resistant or hypervirulent. Two households included multiple colonized participants. Notably, two colonized dogs within household 15 (H15) shared a strain each (ST252 and ST1241) with one coliving human. One dog from H16 shared one PFGE-undistinguishable K. pneumoniae ST17 strain with two humans from different households; however, the antimicrobial susceptibility phenotypes of these three strains differed. Two main virulence genotypes were detected, namely fimH-1 mrkD ycfM entB kfu and fimH-1 mrkD ycfM entB kpn. These results highlight the potential role of dogs as a reservoir of K. pneumoniae to humans and vice versa. Furthermore, to our best knowledge, this is the first report of healthy humans and dogs sharing K. pneumoniae strains that were undistinguishable by PFGE/MLST. |
|---|---|
| Autores principais: | Marques, Cátia Filipa Saraiva |
| Outros Autores: | Belas, Adriana; Aboim, Catarina; Cavaco-Silva, Patrícia; Trigueiro, Graça; Gama, Luis; Pomba, C. |
| Assunto: | Klebsiella pneumoniae animal-human sharing clonal relatedness companion animals dog humans |
| Ano: | 2019 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1866810589082288128 |
|---|---|
| author | Marques, Cátia Filipa Saraiva |
| author2 | Belas, Adriana Aboim, Catarina Cavaco-Silva, Patrícia Trigueiro, Graça Gama, Luis Pomba, C. |
| author2_role | author author author author author author |
| author_facet | Marques, Cátia Filipa Saraiva Belas, Adriana Aboim, Catarina Cavaco-Silva, Patrícia Trigueiro, Graça Gama, Luis Pomba, C. |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Marques, Cátia Filipa Saraiva\",\"Person.identifier.orcid\":\"0000-0001-9648-6380\"},{\"Person.name\":\"Belas, Adriana\",\"Person.identifier.orcid\":\"0000-0001-6564-6839\"},{\"Person.name\":\"Aboim, Catarina\"},{\"Person.name\":\"Cavaco-Silva, Patrícia\"},{\"Person.name\":\"Trigueiro, Graça\"},{\"Person.name\":\"Gama, Luis\",\"Person.identifier.orcid\":\"0000-0003-3894-3488\"},{\"Person.name\":\"Pomba, C.\",\"Person.identifier.orcid\":\"0000-0002-0504-6820\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Marques, Cátia Filipa Saraiva Belas, Adriana Aboim, Catarina Cavaco-Silva, Patrícia Trigueiro, Graça Gama, Luis Pomba, C. |
| datacite.date.Accepted.fl_str_mv | 2019-04-03T00:00:00Z |
| datacite.date.available.fl_str_mv | 2021-09-29T23:16:22Z |
| datacite.date.embargoed.fl_str_mv | 2021-09-29T23:16:22Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Klebsiella pneumoniae animal-human sharing clonal relatedness companion animals dog humans |
| datacite.titles.title.fl_str_mv | Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Marques, Cátia Filipa Saraiva Belas, Adriana Aboim, Catarina Cavaco-Silva, Patrícia Trigueiro, Graça Gama, Luis Pomba, C. |
| dc.date.Accepted.fl_str_mv | 2019-04-03T00:00:00Z |
| dc.date.available.fl_str_mv | 2021-09-29T23:16:22Z |
| dc.date.embargoed.fl_str_mv | 2021-09-29T23:16:22Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.5/22110 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | American Society for Microbiology |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Klebsiella pneumoniae animal-human sharing clonal relatedness companion animals dog humans |
| dc.title.fl_str_mv | Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | This study aimed to characterize the fecal colonization and sharing of Klebsiella pneumoniae strains between companion animals and humans living in close contact. Fecal samples were collected from 50 healthy participants (24 humans, 18 dogs, and 8 cats) belonging to 18 households. Samples were plated onto MacConkey agar (MCK) plates with and without cefotaxime or meropenem supplementation. Up to five K. pneumoniae colonies per participant were compared by pulsed-field gel electrophoresis (PFGE) after XbaI restriction. K. pneumoniae strains with unique pulse types from each participant were characterized for antimicrobial susceptibility, virulence genes, and multilocus sequence type (MLST). Fecal K. pneumoniae pulse types were compared to those of clinical K. pneumoniae strains from animal and human patients with urinary tract infections (n = 104). K. pneumoniae colonization was detected in nonsupplemented MCK in around 38% of dogs (n = 7) and humans (n = 9). K. pneumoniae strains isolated from dogs belonged to sequence type 17 (ST17), ST188, ST252, ST281, ST423, ST1093, ST1241, ST3398, and ST3399. None of the K. pneumoniae strains were multidrug resistant or hypervirulent. Two households included multiple colonized participants. Notably, two colonized dogs within household 15 (H15) shared a strain each (ST252 and ST1241) with one coliving human. One dog from H16 shared one PFGE-undistinguishable K. pneumoniae ST17 strain with two humans from different households; however, the antimicrobial susceptibility phenotypes of these three strains differed. Two main virulence genotypes were detected, namely fimH-1 mrkD ycfM entB kfu and fimH-1 mrkD ycfM entB kpn. These results highlight the potential role of dogs as a reservoir of K. pneumoniae to humans and vice versa. Furthermore, to our best knowledge, this is the first report of healthy humans and dogs sharing K. pneumoniae strains that were undistinguishable by PFGE/MLST. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/f767d759-8ca8-451d-b662-27de062d455b/download |
| id | ul_e82ee1b86dfd0e2de949d55f32f48bf3 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.5/22110 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10400.5/22110 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Marques, Cátia Filipa Saraiva Marques, Cátia Filipa Saraiva https://www.ciencia-id.pt/8015-B7EE-5FFC 8015-B7EE-5FFC http://orcid.org/0000-0001-9648-6380 0000-0001-9648-6380 Belas, Adriana Belas, Adriana https://www.ciencia-id.pt/4D1D-1718-6FFF 4D1D-1718-6FFF http://orcid.org/0000-0001-6564-6839 0000-0001-6564-6839 Aboim, Catarina Cavaco-Silva, Patrícia Trigueiro, Graça Gama, Luis Gama, Luis https://www.ciencia-id.pt/C71D-140F-E876 C71D-140F-E876 http://orcid.org/0000-0003-3894-3488 0000-0003-3894-3488 Pomba, C. Pomba, C. https://www.ciencia-id.pt/DA1D-20A8-7F3C DA1D-20A8-7F3C http://orcid.org/0000-0002-0504-6820 0000-0002-0504-6820 |
| publishDate | 2019 |
| publisher.none.fl_str_mv | American Society for Microbiology |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engAmerican Society for Microbiologypt_PTThis study aimed to characterize the fecal colonization and sharing of Klebsiella pneumoniae strains between companion animals and humans living in close contact. Fecal samples were collected from 50 healthy participants (24 humans, 18 dogs, and 8 cats) belonging to 18 households. Samples were plated onto MacConkey agar (MCK) plates with and without cefotaxime or meropenem supplementation. Up to five K. pneumoniae colonies per participant were compared by pulsed-field gel electrophoresis (PFGE) after XbaI restriction. K. pneumoniae strains with unique pulse types from each participant were characterized for antimicrobial susceptibility, virulence genes, and multilocus sequence type (MLST). Fecal K. pneumoniae pulse types were compared to those of clinical K. pneumoniae strains from animal and human patients with urinary tract infections (n = 104). K. pneumoniae colonization was detected in nonsupplemented MCK in around 38% of dogs (n = 7) and humans (n = 9). K. pneumoniae strains isolated from dogs belonged to sequence type 17 (ST17), ST188, ST252, ST281, ST423, ST1093, ST1241, ST3398, and ST3399. None of the K. pneumoniae strains were multidrug resistant or hypervirulent. Two households included multiple colonized participants. Notably, two colonized dogs within household 15 (H15) shared a strain each (ST252 and ST1241) with one coliving human. One dog from H16 shared one PFGE-undistinguishable K. pneumoniae ST17 strain with two humans from different households; however, the antimicrobial susceptibility phenotypes of these three strains differed. Two main virulence genotypes were detected, namely fimH-1 mrkD ycfM entB kfu and fimH-1 mrkD ycfM entB kpn. These results highlight the potential role of dogs as a reservoir of K. pneumoniae to humans and vice versa. Furthermore, to our best knowledge, this is the first report of healthy humans and dogs sharing K. pneumoniae strains that were undistinguishable by PFGE/MLST.application/pdfpt_PTEvidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humansPersonalMarques, Cátia Filipa SaraivaDSpacehttp://dspace.org/items/2948253e-c3e1-439d-9c74-1356168bc30aDSpacehttp://dspace.org/items/2948253e-c3e1-439d-9c74-1356168bc30aMarquesCátiaCiência IDhttps://www.ciencia-id.pt8015-B7EE-5FFCORCIDhttp://orcid.org0000-0001-9648-6380Scopus Author IDhttps://www.scopus.com55433369800PersonalBelas, AdrianaDSpacehttp://dspace.org/items/99abed93-3af0-40c8-a102-b4143c1c3d06DSpacehttp://dspace.org/items/99abed93-3af0-40c8-a102-b4143c1c3d06Inácio BelasAdriana de JesusCiência IDhttps://www.ciencia-id.pt4D1D-1718-6FFFORCIDhttp://orcid.org0000-0001-6564-6839Aboim, CatarinaCavaco-Silva, PatríciaTrigueiro, GraçaPersonalGama, LuisDSpacehttp://dspace.org/items/e6ff858c-9615-437f-98a7-b61b4362834cDSpacehttp://dspace.org/items/e6ff858c-9615-437f-98a7-b61b4362834cGamaLuisCiência IDhttps://www.ciencia-id.ptC71D-140F-E876ORCIDhttp://orcid.org0000-0003-3894-3488Researcher IDhttps://www.researcherid.comN-4172-2018Scopus Author IDhttps://www.scopus.com36027387300PersonalPomba, C.DSpacehttp://dspace.org/items/53f8cdd6-0030-47fd-9881-a78557579f70DSpacehttp://dspace.org/items/53f8cdd6-0030-47fd-9881-a78557579f70Matias Ferreira PombaMaria ConstançaCiência IDhttps://www.ciencia-id.ptDA1D-20A8-7F3CORCIDhttp://orcid.org0000-0002-0504-6820HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptDOIIsPartOf10.1128/JCM.01537-182021-09-29T23:16:22Z2019-04-032019-04-03T00:00:00ZHandlehttp://hdl.handle.net/10400.5/22110http://purl.org/coar/access_right/c_abf2open accessKlebsiella pneumoniaeanimal-human sharingclonal relatednesscompanion animalsdoghumans924821 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2019-04-03http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/f767d759-8ca8-451d-b662-27de062d455b/downloadJournal of Clinical Microbiologyv. 57 (6)e01537-18WASHINGTON |
| spellingShingle | Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans Marques, Cátia Filipa Saraiva Klebsiella pneumoniae animal-human sharing clonal relatedness companion animals dog humans |
| status | SINGLETON |
| subject.fl_str_mv | Klebsiella pneumoniae animal-human sharing clonal relatedness companion animals dog humans |
| title | Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans |
| title_full | Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans |
| title_fullStr | Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans |
| title_full_unstemmed | Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans |
| title_short | Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans |
| title_sort | Evidence of sharing of Klebsiella pneumoniae strains between healthy companion animals and cohabiting humans |
| topic | Klebsiella pneumoniae animal-human sharing clonal relatedness companion animals dog humans |
| topic_facet | Klebsiella pneumoniae animal-human sharing clonal relatedness companion animals dog humans |
| url | http://hdl.handle.net/10400.5/22110 |
| visible | 1 |