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Antibiotic resistance and virulence encoding genes are present in bacteria isolated from water and street food sold in Mozambique

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Resumo:The main objective of this study was, to evaluate the microbiological quality and safety of water and RTE foods sold on the streets of Maputo. For this, 83 RTE street food samples from 83 different vendors and 116 water samples from different sources were analyzed. Selected bacterial isolates were molecularly identified and characterized. High levels of unsatisfactory samples were found in both hot (76.7%) and cold (75%) foods. Based on staphylococcal counts, approximately 25% of the food samples were unsatisfactory/potentially hazardous. A high level of fecal contamination was detected in all types of water samples. In Escherichia coli isolates from food and water, the highest percentages of resistance were for the ß-lactams imipenem (35.5 and 39.3%, respectively) and ampicillin (39.3 and 46.4%, respectively). Multidrug resistance was observed in 31.3% of the isolates, being higher in water isolates (45.5%) than food isolates (19.2%). ESBL was the most frequent (57.7%) antibiotic resistance gene among those encoding β-lactamases, while tetA was the most frequent (50%) among non-β-lactamase genes. Furthermore, 73% of the multidrug-resistant E. coli isolates also had virulence genes. The most frequent virulence gene was sec (30.3%) followed by the hlb (24.2%) and sak and sed genes (12.5% each). Regarding the presence of antibiotic resistance genes in Staphylococcus, blaZ (penicillin resistance) was the most frequent (74.2%) followed by mecA (methicillin resistance) and vancA (vancomycin resistance) genes (43.9% and 42.4%, respectively. Staphylococcus isolates had a high ability to form biofilms on different materials and these biofilms were resistant to high concentrations of methicillin (32 g/ml). The results reported here show that water and food sold on the streets of Maputo, Mozambique, pose a potential risk to public health.
Autores principais:Salamandane, Acácio Rosse
Assunto:RTE street food and street water Escherichia coli Staphylococcus virulence and antibiotic resistance genes multidrug resistance água e alimentos de rua prontos para consumo (RTE street food) Escherichia coli Staphylococcus spp. genes de virulência e de resistência a antibióticos multirresistência
Ano:2024
País:Portugal
Tipo de documento:tese de doutoramento
Tipo de acesso:acesso aberto
Instituição associada:Universidade de Lisboa
Idioma:inglês
Origem:Repositório da Universidade de Lisboa

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