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Dietary Ulva lactuca and CAZyme supplementation improve serum biochemical profile and hepatic composition of weaned piglets

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Resumo:Ulva lactuca is a seaweed with antinutritional cell wall for monogastrics. Carbohydrate-Active enZymes (CAZymes) supplementation can potentially cause its disruption. This study evaluates four diets: Ctrl—control diet; UL—control + 7% U. lactuca (wild caught, powdered form); ULR—UL + 0.005% Rovabio® Excel AP; ULU—UL + 0.01% ulvan lyase on piglets’ haematologic and serologic profiles, hepatic lipids and minerals. White blood cells and lymphocytes reached the highest values in piglets fed UL compared to control, and to control and ULR; respectively (P < 0.05). IgG levels were boosted by seaweed incorporation compared to control (P = 0.015). The glycaemic homeostasis was assured by the seaweed inclusion. Dietary seaweed decreased serum lipids (P < 0.001), with the exception of ULU, due to HDL-cholesterol increase (P < 0.001). Cortisol was decreased in ULR and ULU (P < 0.001). No systemic inflammation was observed (P > 0.05). While hepatic n-3 PUFA increased in piglets fed with seaweed diets due to increment of beneficial 22:5n-3 and 22:6n-3 fatty acids (P < 0.05), the opposite occurred for n-6 PUFA, PUFA/SFA and n-6/n-3 ratios (P < 0.05). Hepatic pigments were unchanged (P > 0.05). ULR reduced α-tocopherol levels (P = 0.036) and increased serum potassium levels (P < 0.001) compared to control. Seaweed contributed to overcome piglets’ weaning stress, with some benefits of including CAZyme supplementation.
Autores principais:Ribeiro, David M.
Outros Autores:Lopes, Paula A.; Pinto, Rui M. A.; Pestana, José M.; Costa, Mónica M.; Alfaia, Cristina M.; Mourato, Miguel; Almeida, André; Freire, João; Prates, José A. M.
Ano:2023
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Universidade de Lisboa
Idioma:inglês
Origem:Repositório da Universidade de Lisboa
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author Ribeiro, David M.
author2 Lopes, Paula A.
Pinto, Rui M. A.
Pestana, José M.
Costa, Mónica M.
Alfaia, Cristina M.
Mourato, Miguel
Almeida, André
Freire, João
Prates, José A. M.
author2_role author
author
author
author
author
author
author
author
author
author_facet Ribeiro, David M.
Lopes, Paula A.
Pinto, Rui M. A.
Pestana, José M.
Costa, Mónica M.
Alfaia, Cristina M.
Mourato, Miguel
Almeida, André
Freire, João
Prates, José A. M.
author_role author
contributor_name_str_mv Repositório Científico de Acesso Aberto da ULisboa
country_str PT
creators_json_txt [{\"Person.name\":\"Ribeiro, David M.\"},{\"Person.name\":\"Lopes, Paula A.\"},{\"Person.name\":\"Pinto, Rui M. A.\"},{\"Person.name\":\"Pestana, José M.\"},{\"Person.name\":\"Costa, Mónica M.\"},{\"Person.name\":\"Alfaia, Cristina M.\"},{\"Person.name\":\"Mourato, Miguel\",\"Person.identifier.orcid\":\"0000-0003-4843-1064\"},{\"Person.name\":\"Almeida, André\",\"Person.identifier.orcid\":\"0000-0002-7810-3988\"},{\"Person.name\":\"Freire, João\",\"Person.identifier.orcid\":\"0000-0002-1811-4378\"},{\"Person.name\":\"Prates, José A. M.\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Repositório Científico de Acesso Aberto da ULisboa
datacite.creators.creator.creatorName.fl_str_mv Ribeiro, David M.
Lopes, Paula A.
Pinto, Rui M. A.
Pestana, José M.
Costa, Mónica M.
Alfaia, Cristina M.
Mourato, Miguel
Almeida, André
Freire, João
Prates, José A. M.
datacite.date.Accepted.fl_str_mv 2023-12-01T00:00:00Z
datacite.date.available.fl_str_mv 2024-07-22T18:33:16Z
datacite.date.embargoed.fl_str_mv 2024-07-22T18:33:16Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.titles.title.fl_str_mv Dietary Ulva lactuca and CAZyme supplementation improve serum biochemical profile and hepatic composition of weaned piglets
dc.contributor.none.fl_str_mv Repositório Científico de Acesso Aberto da ULisboa
dc.creator.none.fl_str_mv Ribeiro, David M.
Lopes, Paula A.
Pinto, Rui M. A.
Pestana, José M.
Costa, Mónica M.
Alfaia, Cristina M.
Mourato, Miguel
Almeida, André
Freire, João
Prates, José A. M.
dc.date.Accepted.fl_str_mv 2023-12-01T00:00:00Z
dc.date.available.fl_str_mv 2024-07-22T18:33:16Z
dc.date.embargoed.fl_str_mv 2024-07-22T18:33:16Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10400.5/31340
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv Nature
dc.rights.cclincense.fl_str_mv http://creativecommons.org/licenses/by/4.0/
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.title.fl_str_mv Dietary Ulva lactuca and CAZyme supplementation improve serum biochemical profile and hepatic composition of weaned piglets
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description Ulva lactuca is a seaweed with antinutritional cell wall for monogastrics. Carbohydrate-Active enZymes (CAZymes) supplementation can potentially cause its disruption. This study evaluates four diets: Ctrl—control diet; UL—control + 7% U. lactuca (wild caught, powdered form); ULR—UL + 0.005% Rovabio® Excel AP; ULU—UL + 0.01% ulvan lyase on piglets’ haematologic and serologic profiles, hepatic lipids and minerals. White blood cells and lymphocytes reached the highest values in piglets fed UL compared to control, and to control and ULR; respectively (P < 0.05). IgG levels were boosted by seaweed incorporation compared to control (P = 0.015). The glycaemic homeostasis was assured by the seaweed inclusion. Dietary seaweed decreased serum lipids (P < 0.001), with the exception of ULU, due to HDL-cholesterol increase (P < 0.001). Cortisol was decreased in ULR and ULU (P < 0.001). No systemic inflammation was observed (P > 0.05). While hepatic n-3 PUFA increased in piglets fed with seaweed diets due to increment of beneficial 22:5n-3 and 22:6n-3 fatty acids (P < 0.05), the opposite occurred for n-6 PUFA, PUFA/SFA and n-6/n-3 ratios (P < 0.05). Hepatic pigments were unchanged (P > 0.05). ULR reduced α-tocopherol levels (P = 0.036) and increased serum potassium levels (P < 0.001) compared to control. Seaweed contributed to overcome piglets’ weaning stress, with some benefits of including CAZyme supplementation.
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eu_rights_str_mv openAccess
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id ul_ec2e776c8a07ff28947bae625ec8beec
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institution Universidade de Lisboa
instname_str Universidade de Lisboa
language eng
network_acronym_str ul
network_name_str Repositório da Universidade de Lisboa
oai_identifier_str oai:repositorio.ulisboa.pt:10400.5/31340
organization_str_mv urn:organizationAcronym:ul
person_str_mv Ribeiro, David M.
Lopes, Paula A.
Pinto, Rui M. A.
Pestana, José M.
Costa, Mónica M.
Alfaia, Cristina M.
Mourato, Miguel
Mourato, Miguel
https://www.ciencia-id.pt/3D1E-4003-BF64
3D1E-4003-BF64
http://orcid.org/0000-0003-4843-1064
0000-0003-4843-1064
Almeida, André
Almeida, André
https://www.ciencia-id.pt/1E14-92CC-B163
1E14-92CC-B163
http://orcid.org/0000-0002-7810-3988
0000-0002-7810-3988
Freire, João
Freire, João
https://www.ciencia-id.pt/871A-F6C8-60FE
871A-F6C8-60FE
http://orcid.org/0000-0002-1811-4378
0000-0002-1811-4378
Prates, José A. M.
publishDate 2023
publisher.none.fl_str_mv Nature
reponame_str Repositório da Universidade de Lisboa
repository_id_str urn:repositoryAcronym:ul
service_str_mv urn:repositoryAcronym:ul
spelling engNaturept_PTUlva lactuca is a seaweed with antinutritional cell wall for monogastrics. Carbohydrate-Active enZymes (CAZymes) supplementation can potentially cause its disruption. This study evaluates four diets: Ctrl—control diet; UL—control + 7% U. lactuca (wild caught, powdered form); ULR—UL + 0.005% Rovabio® Excel AP; ULU—UL + 0.01% ulvan lyase on piglets’ haematologic and serologic profiles, hepatic lipids and minerals. White blood cells and lymphocytes reached the highest values in piglets fed UL compared to control, and to control and ULR; respectively (P < 0.05). IgG levels were boosted by seaweed incorporation compared to control (P = 0.015). The glycaemic homeostasis was assured by the seaweed inclusion. Dietary seaweed decreased serum lipids (P < 0.001), with the exception of ULU, due to HDL-cholesterol increase (P < 0.001). Cortisol was decreased in ULR and ULU (P < 0.001). No systemic inflammation was observed (P > 0.05). While hepatic n-3 PUFA increased in piglets fed with seaweed diets due to increment of beneficial 22:5n-3 and 22:6n-3 fatty acids (P < 0.05), the opposite occurred for n-6 PUFA, PUFA/SFA and n-6/n-3 ratios (P < 0.05). Hepatic pigments were unchanged (P > 0.05). ULR reduced α-tocopherol levels (P = 0.036) and increased serum potassium levels (P < 0.001) compared to control. Seaweed contributed to overcome piglets’ weaning stress, with some benefits of including CAZyme supplementation.application/pdfpt_PTDietary Ulva lactuca and CAZyme supplementation improve serum biochemical profile and hepatic composition of weaned pigletsRibeiro, David M.Lopes, Paula A.Pinto, Rui M. A.Pestana, José M.Costa, Mónica M.Alfaia, Cristina M.PersonalMourato, MiguelDSpacehttp://dspace.org/items/f3e8b80c-0f8e-42bb-a85c-078de348063bDSpacehttp://dspace.org/items/f3e8b80c-0f8e-42bb-a85c-078de348063bMouratoMiguelCiência IDhttps://www.ciencia-id.pt3D1E-4003-BF64ORCIDhttp://orcid.org0000-0003-4843-1064Researcher IDhttps://www.researcherid.comB-6904-2009Scopus Author IDhttps://www.scopus.com56370610300PersonalAlmeida, AndréDSpacehttp://dspace.org/items/722b3039-6553-4059-8e11-d5eaf113a8cdDSpacehttp://dspace.org/items/722b3039-6553-4059-8e11-d5eaf113a8cdMartinho de AlmeidaAndréCiência IDhttps://www.ciencia-id.pt1E14-92CC-B163ORCIDhttp://orcid.org0000-0002-7810-3988PersonalFreire, JoãoDSpacehttp://dspace.org/items/99660917-13cd-47e0-bf7d-667497ebcfbeDSpacehttp://dspace.org/items/99660917-13cd-47e0-bf7d-667497ebcfbeBengala FreireJoão PedroCiência IDhttps://www.ciencia-id.pt871A-F6C8-60FEORCIDhttp://orcid.org0000-0002-1811-4378Prates, José A. M.HostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptDOIIsPartOf10.1038/s41598-023-36008-42024-07-22T18:33:16Z2023-122023-12-01T00:00:00ZHandlehttp://hdl.handle.net/10400.5/31340http://purl.org/coar/access_right/c_abf2open access1451692 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2023-12http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/a26dc751-6dd6-449f-be5c-e22cf89a0d79/downloadScientific Reports131Article number 8784
spellingShingle Dietary Ulva lactuca and CAZyme supplementation improve serum biochemical profile and hepatic composition of weaned piglets
Ribeiro, David M.
status SINGLETON
title Dietary Ulva lactuca and CAZyme supplementation improve serum biochemical profile and hepatic composition of weaned piglets
title_full Dietary Ulva lactuca and CAZyme supplementation improve serum biochemical profile and hepatic composition of weaned piglets
title_fullStr Dietary Ulva lactuca and CAZyme supplementation improve serum biochemical profile and hepatic composition of weaned piglets
title_full_unstemmed Dietary Ulva lactuca and CAZyme supplementation improve serum biochemical profile and hepatic composition of weaned piglets
title_short Dietary Ulva lactuca and CAZyme supplementation improve serum biochemical profile and hepatic composition of weaned piglets
title_sort Dietary Ulva lactuca and CAZyme supplementation improve serum biochemical profile and hepatic composition of weaned piglets
url http://hdl.handle.net/10400.5/31340
visible 1