Publicação
Efeitos inibitórios do hidroximetilfurfural na fermentação alcoólica: avaliação por citometria de fluxo
| Resumo: | The growing importance of biofuels, such as bioethanol, in today's society as an alternative to oil, was the driving force of this work. The lack of information about the effect of a major alcoholic fermentation inhibitor present in lignocellulosic hydrolysates towards a yeast of the genus Saccharomyces (the most widely used in bioethanol production) justifies the interest and relevance of this study that aim at assessing the inhibitory effect of hydroxymethylfurfural (HMF) during alcoholic fermentation by the yeast Saccharomyces carlsbergensis. The physiological effects evaluation was performed by multiparametric flow cytometry by detecting changes in the cytoplasmic membrane, lipids composition and oxygen reactive species (ROS) production. It was observed that increasing concentrations of HMF inhibit growth (Ki = 0.188 L/g) and ethanol production although in lesser extent in the latter case (20% reduction with 10 g/L). Only in the presence of 15 g/L it was observed neither growth nor ethanol production. The analysis by multiparameter flow cytometry with the use of specific dyes has proved to be an indispensable tool in this study, showing that increasing concentrations of HMF induce loss of cytoplasmic membrane integrity and polarization, production of ROS and changes in the cellular lipid fraction. |
|---|---|
| Autores principais: | Baptista, Cátia Vanessa Rodrigues |
| Assunto: | bioethanol Saccharomyces carlsbergensis inhibition hydroxymethylfurfural multiparametric flow cytometry |
| Ano: | 2013 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | português |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1866810816235307008 |
|---|---|
| author | Baptista, Cátia Vanessa Rodrigues |
| author_facet | Baptista, Cátia Vanessa Rodrigues |
| author_role | author |
| contributor_name_str_mv | Silva, Maria Teresa Saraiva Lopes da Passarinho, Paula Cristina Varela Martins, Maria Luísa Louro Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Baptista, Cátia Vanessa Rodrigues\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Silva, Maria Teresa Saraiva Lopes da Passarinho, Paula Cristina Varela Martins, Maria Luísa Louro Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Baptista, Cátia Vanessa Rodrigues |
| datacite.date.Accepted.fl_str_mv | 2013-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2014-04-01T13:37:30Z |
| datacite.date.embargoed.fl_str_mv | 2014-04-01T13:37:30Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | bioethanol Saccharomyces carlsbergensis inhibition hydroxymethylfurfural multiparametric flow cytometry |
| datacite.titles.title.fl_str_mv | Efeitos inibitórios do hidroximetilfurfural na fermentação alcoólica: avaliação por citometria de fluxo |
| dc.contributor.none.fl_str_mv | Silva, Maria Teresa Saraiva Lopes da Passarinho, Paula Cristina Varela Martins, Maria Luísa Louro Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Baptista, Cátia Vanessa Rodrigues |
| dc.date.Accepted.fl_str_mv | 2013-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2014-04-01T13:37:30Z |
| dc.date.embargoed.fl_str_mv | 2014-04-01T13:37:30Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.5/6697 |
| dc.language.none.fl_str_mv | por |
| dc.publisher.none.fl_str_mv | ISA |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | bioethanol Saccharomyces carlsbergensis inhibition hydroxymethylfurfural multiparametric flow cytometry |
| dc.title.fl_str_mv | Efeitos inibitórios do hidroximetilfurfural na fermentação alcoólica: avaliação por citometria de fluxo |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_bdcc |
| description | The growing importance of biofuels, such as bioethanol, in today's society as an alternative to oil, was the driving force of this work. The lack of information about the effect of a major alcoholic fermentation inhibitor present in lignocellulosic hydrolysates towards a yeast of the genus Saccharomyces (the most widely used in bioethanol production) justifies the interest and relevance of this study that aim at assessing the inhibitory effect of hydroxymethylfurfural (HMF) during alcoholic fermentation by the yeast Saccharomyces carlsbergensis. The physiological effects evaluation was performed by multiparametric flow cytometry by detecting changes in the cytoplasmic membrane, lipids composition and oxygen reactive species (ROS) production. It was observed that increasing concentrations of HMF inhibit growth (Ki = 0.188 L/g) and ethanol production although in lesser extent in the latter case (20% reduction with 10 g/L). Only in the presence of 15 g/L it was observed neither growth nor ethanol production. The analysis by multiparameter flow cytometry with the use of specific dyes has proved to be an indispensable tool in this study, showing that increasing concentrations of HMF induce loss of cytoplasmic membrane integrity and polarization, production of ROS and changes in the cellular lipid fraction. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | masterThesis |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/40bf5bc8-f037-41fb-a7b0-2475b6211056/download |
| id | ul_ef52cdcef8eeda7734e032a9e8fc507a |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.5/6697 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | por |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10400.5/6697 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Baptista, Cátia Vanessa Rodrigues |
| publishDate | 2013 |
| publisher.none.fl_str_mv | ISA |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | porISAporThe growing importance of biofuels, such as bioethanol, in today's society as an alternative to oil, was the driving force of this work. The lack of information about the effect of a major alcoholic fermentation inhibitor present in lignocellulosic hydrolysates towards a yeast of the genus Saccharomyces (the most widely used in bioethanol production) justifies the interest and relevance of this study that aim at assessing the inhibitory effect of hydroxymethylfurfural (HMF) during alcoholic fermentation by the yeast Saccharomyces carlsbergensis. The physiological effects evaluation was performed by multiparametric flow cytometry by detecting changes in the cytoplasmic membrane, lipids composition and oxygen reactive species (ROS) production. It was observed that increasing concentrations of HMF inhibit growth (Ki = 0.188 L/g) and ethanol production although in lesser extent in the latter case (20% reduction with 10 g/L). Only in the presence of 15 g/L it was observed neither growth nor ethanol production. The analysis by multiparameter flow cytometry with the use of specific dyes has proved to be an indispensable tool in this study, showing that increasing concentrations of HMF induce loss of cytoplasmic membrane integrity and polarization, production of ROS and changes in the cellular lipid fraction.application/pdfporEfeitos inibitórios do hidroximetilfurfural na fermentação alcoólica: avaliação por citometria de fluxoBaptista, Cátia Vanessa RodriguesSilva, Maria Teresa Saraiva Lopes daPassarinho, Paula Cristina VarelaMartins, Maria Luísa LouroHostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptURNurn:tid:2019215962014-04-01T13:37:30Z20132013-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.5/6697http://purl.org/coar/access_right/c_abf2open accessbioethanolSaccharomyces carlsbergensisinhibitionhydroxymethylfurfuralmultiparametric flow cytometry2902097 bytesliteraturehttp://purl.org/coar/resource_type/c_bdccmaster thesishttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/40bf5bc8-f037-41fb-a7b0-2475b6211056/download |
| spellingShingle | Efeitos inibitórios do hidroximetilfurfural na fermentação alcoólica: avaliação por citometria de fluxo Baptista, Cátia Vanessa Rodrigues bioethanol Saccharomyces carlsbergensis inhibition hydroxymethylfurfural multiparametric flow cytometry |
| status | SINGLETON |
| subject.fl_str_mv | bioethanol Saccharomyces carlsbergensis inhibition hydroxymethylfurfural multiparametric flow cytometry |
| title | Efeitos inibitórios do hidroximetilfurfural na fermentação alcoólica: avaliação por citometria de fluxo |
| title_full | Efeitos inibitórios do hidroximetilfurfural na fermentação alcoólica: avaliação por citometria de fluxo |
| title_fullStr | Efeitos inibitórios do hidroximetilfurfural na fermentação alcoólica: avaliação por citometria de fluxo |
| title_full_unstemmed | Efeitos inibitórios do hidroximetilfurfural na fermentação alcoólica: avaliação por citometria de fluxo |
| title_short | Efeitos inibitórios do hidroximetilfurfural na fermentação alcoólica: avaliação por citometria de fluxo |
| title_sort | Efeitos inibitórios do hidroximetilfurfural na fermentação alcoólica: avaliação por citometria de fluxo |
| topic | bioethanol Saccharomyces carlsbergensis inhibition hydroxymethylfurfural multiparametric flow cytometry |
| topic_facet | bioethanol Saccharomyces carlsbergensis inhibition hydroxymethylfurfural multiparametric flow cytometry |
| url | http://hdl.handle.net/10400.5/6697 |
| visible | 1 |