Publicação
A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination
| Resumo: | In eukaryotes, the production of mature messenger RNA that exits the nucleus to be translated into protein in the cytoplasm requires precise and extensive modification of the nascent transcript. Any failure that compromises the integrity of an mRNA may cause its retention in the nucleus and trigger its degradation. Multiple studies indicate that mRNAs with processing defects accumulate in nuclear foci or ‘dots’ located near the site of transcription, but how exactly are defective RNAs recognized and tethered is still unknown. Here, we present evidence suggesting that unprocessed β-globin transcripts render RNA polymerase II (Pol II) incompetent for termination and that this quality control process requires the integrity of the nuclear exosome. Our results show that unprocessed pre-mRNAs remain tethered to the DNA template in association with Pol II, in an Rrp6-dependent manner. This reveals an unprecedented link between nuclear RNA surveillance, the exosome and Pol II transcriptional termination. |
|---|---|
| Autores principais: | Almeida, Sérgio F. de |
| Outros Autores: | García-Sacristán, Ana; Custódio, Noélia; Carmo-Fonseca, Maria |
| Assunto: | Eukaryotes RNA, messenger Exosome RNA polymerase II |
| Ano: | 2010 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade de Lisboa |
| Idioma: | inglês |
| Origem: | Repositório da Universidade de Lisboa |
| _version_ | 1865920851432439808 |
|---|---|
| author | Almeida, Sérgio F. de |
| author2 | García-Sacristán, Ana Custódio, Noélia Carmo-Fonseca, Maria |
| author2_role | author author author |
| author_facet | Almeida, Sérgio F. de Almeida, Sérgio F. de García-Sacristán, Ana Custódio, Noélia Carmo-Fonseca, Maria García-Sacristán, Ana Custódio, Noélia Carmo-Fonseca, Maria |
| author_role | author |
| contributor_name_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Almeida, Sérgio F. de\"},{\"Person.name\":\"García-Sacristán, Ana\"},{\"Person.name\":\"Custódio, Noélia\"},{\"Person.name\":\"Carmo-Fonseca, Maria\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| datacite.creators.creator.creatorName.fl_str_mv | Almeida, Sérgio F. de García-Sacristán, Ana Custódio, Noélia Carmo-Fonseca, Maria |
| datacite.date.Accepted.fl_str_mv | 2010-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2012-05-22T15:34:57Z |
| datacite.date.embargoed.fl_str_mv | 2012-05-22T15:34:57Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Eukaryotes RNA, messenger Exosome RNA polymerase II |
| datacite.titles.title.fl_str_mv | A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination |
| dc.contributor.none.fl_str_mv | Repositório Científico de Acesso Aberto da ULisboa |
| dc.creator.none.fl_str_mv | Almeida, Sérgio F. de García-Sacristán, Ana Custódio, Noélia Carmo-Fonseca, Maria |
| dc.date.Accepted.fl_str_mv | 2010-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2012-05-22T15:34:57Z |
| dc.date.embargoed.fl_str_mv | 2012-05-22T15:34:57Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10451/6360 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Oxford University Press |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Eukaryotes RNA, messenger Exosome RNA polymerase II |
| dc.title.fl_str_mv | A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | In eukaryotes, the production of mature messenger RNA that exits the nucleus to be translated into protein in the cytoplasm requires precise and extensive modification of the nascent transcript. Any failure that compromises the integrity of an mRNA may cause its retention in the nucleus and trigger its degradation. Multiple studies indicate that mRNAs with processing defects accumulate in nuclear foci or ‘dots’ located near the site of transcription, but how exactly are defective RNAs recognized and tethered is still unknown. Here, we present evidence suggesting that unprocessed β-globin transcripts render RNA polymerase II (Pol II) incompetent for termination and that this quality control process requires the integrity of the nuclear exosome. Our results show that unprocessed pre-mRNAs remain tethered to the DNA template in association with Pol II, in an Rrp6-dependent manner. This reveals an unprecedented link between nuclear RNA surveillance, the exosome and Pol II transcriptional termination. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ulisboa.pt/bitstreams/110aca5b-c611-4f6d-aa37-8000dadc9c59/download |
| id | ul_f2ad89efda5325fb2bf8590e33c1e2fc |
| identifier.url.fl_str_mv | http://hdl.handle.net/10451/6360 |
| instacron_str | ul |
| institution | Universidade de Lisboa |
| instname_str | Universidade de Lisboa |
| language | eng |
| network_acronym_str | ul |
| network_name_str | Repositório da Universidade de Lisboa |
| oai_identifier_str | oai:repositorio.ulisboa.pt:10451/6360 |
| organization_str_mv | urn:organizationAcronym:ul |
| person_str_mv | Almeida, Sérgio F. de García-Sacristán, Ana Custódio, Noélia Carmo-Fonseca, Maria |
| publishDate | 2010 |
| publisher.none.fl_str_mv | Oxford University Press |
| reponame_str | Repositório da Universidade de Lisboa |
| repository_id_str | urn:repositoryAcronym:ul |
| service_str_mv | urn:repositoryAcronym:ul |
| spelling | engOxford University PressengIn eukaryotes, the production of mature messenger RNA that exits the nucleus to be translated into protein in the cytoplasm requires precise and extensive modification of the nascent transcript. Any failure that compromises the integrity of an mRNA may cause its retention in the nucleus and trigger its degradation. Multiple studies indicate that mRNAs with processing defects accumulate in nuclear foci or ‘dots’ located near the site of transcription, but how exactly are defective RNAs recognized and tethered is still unknown. Here, we present evidence suggesting that unprocessed β-globin transcripts render RNA polymerase II (Pol II) incompetent for termination and that this quality control process requires the integrity of the nuclear exosome. Our results show that unprocessed pre-mRNAs remain tethered to the DNA template in association with Pol II, in an Rrp6-dependent manner. This reveals an unprecedented link between nuclear RNA surveillance, the exosome and Pol II transcriptional termination.application/pdfengA link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional terminationAlmeida, Sérgio F. deGarcía-Sacristán, AnaCustódio, NoéliaCarmo-Fonseca, MariaHostingInstitutionOrganizationalRepositório Científico de Acesso Aberto da ULisboae-mailmailto:repositorio@reitoria.ulisboa.ptrepositorio@reitoria.ulisboa.ptN/Adoi:10.1093/nar/gkq703URLhttp://nar.oxfordjournals.org/content/38/22/8015.full.pdf+htmlISSNIsPartOf0305-10482012-05-22T15:34:57Z20102010-01-01T00:00:00ZHandlehttp://hdl.handle.net/10451/6360http://purl.org/coar/access_right/c_abf2open accessEukaryotesRNA, messengerExosomeRNA polymerase II5551507 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ulisboa.pt/bitstreams/110aca5b-c611-4f6d-aa37-8000dadc9c59/downloadNucleic Acids Research80158026 |
| spellingShingle | A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination Almeida, Sérgio F. de Eukaryotes RNA, messenger Exosome RNA polymerase II Almeida, Sérgio F. de Eukaryotes RNA, messenger Exosome RNA polymerase II |
| status | SINGLETON |
| subject.fl_str_mv | Eukaryotes RNA, messenger Exosome RNA polymerase II |
| title | A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination |
| title_full | A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination |
| title_fullStr | A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination |
| title_full_unstemmed | A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination |
| title_short | A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination |
| title_sort | A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination |
| topic | Eukaryotes RNA, messenger Exosome RNA polymerase II |
| topic_facet | Eukaryotes RNA, messenger Exosome RNA polymerase II |
| url | http://hdl.handle.net/10451/6360 |
| visible | 1 |