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Effect of biochar supplementation on recycled manure solids microbiome and E. coli composition

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Resumo:Antibiotic resistance is one of the main concerns of the scientific community for the future. There is a risk that antibiotic resistance can be disseminated through food production, namely by food consumption and/or direct contact with animals. Recycled manure solids are a kind of bed used for cows that has several advantages for the animal’s health and the producer's profit but has been associated with higher percentages of pathogens, which poses a risk to public health. Biochar is proposed as a possible sustainable solution to reduce pathogenic bacteria in this kind of bed. The goal of this work was to evaluate how the supplementation of recycled manure solids bedding with different percentages of biochar influence its Escherichia coli composition as well as to characterize the E. coli strains and evaluate bacterial diversity in the samples recurring to metagenomics. Samples of recycled manure solids were collected during the wet (April- May) and dry (JuneJuly) seasons and supplemented with 2.5%, 5%, and 10% biochar. From these samples E. coli was isolated and characterized and a metagenomics study was also performed. Recycled manure solids supplementation with biochar reduced E. coli counts for all wet season samples and for the dry season samples supplemented with 5% biochar. From the 42 representative isolates analyzed, 19% (n=8) showed resistance to ampicillin and 9.5% (n=4) to oxytetracycline, 31% (n=13) were biofilm-producers and all isolates presented a weak alpha-hemolytic activity. Moreover, the metagenomic analysis revealed that biochar supplementation reduced the relative abundance of some important pathogens for dairy cows. Further research is needed to assess biochar's broader impact on these pathogens and its implications for overall cow health.
Autores principais:Esteves, Ana Filipa Marques do Carmo Correia
Assunto:Resistência a antibióticos Produção animal “Recycled manure solids bedding” Biochar Escherichia coli Análise metagenómica Teses de mestrado - 2024
Ano:2024
País:Portugal
Tipo de documento:dissertação de mestrado
Tipo de acesso:acesso embargado
Instituição associada:Universidade de Lisboa
Idioma:inglês
Origem:Repositório da Universidade de Lisboa

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