Publicação
Bacterial community dynamics within an aerobic granular sludge reactor treating wastewater loaded with pharmaceuticals
| Resumo: | Pharmaceuticals are micropollutants often present in wastewater treatment systems. In this study, the potential impact of such micropollutants on the bacterial population within aerobic granular sludge (AGS) bioreactor was investigated. The AGS bacterial community structure and composition were accessed combining DGGE fingerprinting and barcoded pyrosequencing analysis. Both revealed the existence of a dynamic bacterial community, independently of the pharmaceuticals presence. The AGS microbiome at both phylum and class levels varied over time and, after stopping pharmaceuticals feeding, the bacterial community did not return to its initial composition. Nevertheless, most of the assigned OTUs were present throughout the different operational phases. This core microbiome, represented by over 72% of the total sequences in each phase, probably played an important role in biological removal processes, avoiding their failure during the disturbance period. Quantitative-PCR revealed that pharmaceuticals load led to gradual changes on the abundance of ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB) and polyphosphate-accumulating organisms (PAO) but their persistence during that phase demonstrated the resilience of such bacterial groups. AGS microbiome changed over time but a core community was maintained, probably ensuring the accomplishment of the main biological removal processes. |
|---|---|
| Autores principais: | Amorim, Catarina L. |
| Outros Autores: | Alves, Marta; Castro, Paula M.L.; Henriques, Isabel |
| Assunto: | Aerobic granular sludge Microbiome analysis 454-pyrosequencing PCR-DGGE qPCR |
| Ano: | 2018 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade Católica Portuguesa |
| Idioma: | inglês |
| Origem: | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| _version_ | 1866895781090295808 |
|---|---|
| author | Amorim, Catarina L. |
| author2 | Alves, Marta Castro, Paula M.L. Henriques, Isabel |
| author2_role | author author author |
| author_facet | Amorim, Catarina L. Alves, Marta Castro, Paula M.L. Henriques, Isabel |
| author_role | author |
| contributor_name_str_mv | Veritati |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Amorim, Catarina L.\",\"Person.identifier.orcid\":\"0000-0002-6756-552X\"},{\"Person.name\":\"Alves, Marta\",\"Person.identifier.orcid\":\"0000-0003-1060-058X\"},{\"Person.name\":\"Castro, Paula M.L.\",\"Person.identifier.orcid\":\"0000-0001-8841-6606\"},{\"Person.name\":\"Henriques, Isabel\",\"Person.identifier.orcid\":\"0000-0001-7717-4939\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Veritati |
| datacite.creators.creator.creatorName.fl_str_mv | Amorim, Catarina L. Alves, Marta Castro, Paula M.L. Henriques, Isabel |
| datacite.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-09-13T11:59:03Z |
| datacite.date.embargoed.fl_str_mv | 2018-09-13T11:59:03Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Aerobic granular sludge Microbiome analysis 454-pyrosequencing PCR-DGGE qPCR |
| datacite.titles.title.fl_str_mv | Bacterial community dynamics within an aerobic granular sludge reactor treating wastewater loaded with pharmaceuticals |
| dc.contributor.none.fl_str_mv | Veritati |
| dc.creator.none.fl_str_mv | Amorim, Catarina L. Alves, Marta Castro, Paula M.L. Henriques, Isabel |
| dc.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-09-13T11:59:03Z |
| dc.date.embargoed.fl_str_mv | 2018-09-13T11:59:03Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.14/25685 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Aerobic granular sludge Microbiome analysis 454-pyrosequencing PCR-DGGE qPCR |
| dc.title.fl_str_mv | Bacterial community dynamics within an aerobic granular sludge reactor treating wastewater loaded with pharmaceuticals |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Pharmaceuticals are micropollutants often present in wastewater treatment systems. In this study, the potential impact of such micropollutants on the bacterial population within aerobic granular sludge (AGS) bioreactor was investigated. The AGS bacterial community structure and composition were accessed combining DGGE fingerprinting and barcoded pyrosequencing analysis. Both revealed the existence of a dynamic bacterial community, independently of the pharmaceuticals presence. The AGS microbiome at both phylum and class levels varied over time and, after stopping pharmaceuticals feeding, the bacterial community did not return to its initial composition. Nevertheless, most of the assigned OTUs were present throughout the different operational phases. This core microbiome, represented by over 72% of the total sequences in each phase, probably played an important role in biological removal processes, avoiding their failure during the disturbance period. Quantitative-PCR revealed that pharmaceuticals load led to gradual changes on the abundance of ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB) and polyphosphate-accumulating organisms (PAO) but their persistence during that phase demonstrated the resilience of such bacterial groups. AGS microbiome changed over time but a core community was maintained, probably ensuring the accomplishment of the main biological removal processes. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ucp.pt/bitstreams/14759388-67a2-4d6d-9d7d-4b9840b25f9e/download |
| funding.funder.alternateName_str_mv | FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 3599-PPCDT |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10400.14/25685 |
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| institution | Universidade Católica Portuguesa |
| instname_str | Universidade Católica Portuguesa |
| language | eng |
| network_acronym_str | veritati |
| network_name_str | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| oai_identifier_str | oai:repositorio.ucp.pt:10400.14/25685 |
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| person_str_mv | Amorim, Catarina L. Amorim, Catarina L. https://www.ciencia-id.pt/791E-5D42-9B68 791E-5D42-9B68 http://orcid.org/0000-0002-6756-552X 0000-0002-6756-552X Alves, Marta Alves, Marta https://www.ciencia-id.pt/D214-4F83-1445 D214-4F83-1445 http://orcid.org/0000-0003-1060-058X 0000-0003-1060-058X Castro, Paula M.L. Castro, Paula M.L. https://www.ciencia-id.pt/7C1F-6C72-354A 7C1F-6C72-354A http://orcid.org/0000-0001-8841-6606 0000-0001-8841-6606 Henriques, Isabel Henriques, Isabel https://www.ciencia-id.pt/F516-BFA6-1588 F516-BFA6-1588 http://orcid.org/0000-0001-7717-4939 0000-0001-7717-4939 |
| publishDate | 2018 |
| publisher.none.fl_str_mv | Elsevier |
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| spelling | engElsevierpt_PTPharmaceuticals are micropollutants often present in wastewater treatment systems. In this study, the potential impact of such micropollutants on the bacterial population within aerobic granular sludge (AGS) bioreactor was investigated. The AGS bacterial community structure and composition were accessed combining DGGE fingerprinting and barcoded pyrosequencing analysis. Both revealed the existence of a dynamic bacterial community, independently of the pharmaceuticals presence. The AGS microbiome at both phylum and class levels varied over time and, after stopping pharmaceuticals feeding, the bacterial community did not return to its initial composition. Nevertheless, most of the assigned OTUs were present throughout the different operational phases. This core microbiome, represented by over 72% of the total sequences in each phase, probably played an important role in biological removal processes, avoiding their failure during the disturbance period. Quantitative-PCR revealed that pharmaceuticals load led to gradual changes on the abundance of ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB) and polyphosphate-accumulating organisms (PAO) but their persistence during that phase demonstrated the resilience of such bacterial groups. AGS microbiome changed over time but a core community was maintained, probably ensuring the accomplishment of the main biological removal processes.application/pdfpt_PTBacterial community dynamics within an aerobic granular sludge reactor treating wastewater loaded with pharmaceuticalsPersonalAmorim, Catarina L.DSpacehttp://dspace.org/items/17a25a6a-22d5-40bc-9183-0c81b79a89bfDSpacehttp://dspace.org/items/17a25a6a-22d5-40bc-9183-0c81b79a89bfAmorimCatarina L.Ciência IDhttps://www.ciencia-id.pt791E-5D42-9B68ORCIDhttp://orcid.org0000-0002-6756-552XResearcher IDhttps://www.researcherid.comK-6091-2014Scopus Author IDhttps://www.scopus.com36896692500Scopus Author IDhttps://www.scopus.com36896692500PersonalAlves, MartaDSpacehttp://dspace.org/items/51db2775-48fe-4e93-b9c9-a1e6f5e1515bDSpacehttp://dspace.org/items/51db2775-48fe-4e93-b9c9-a1e6f5e1515bAlvesMartaCiência IDhttps://www.ciencia-id.ptD214-4F83-1445ORCIDhttp://orcid.org0000-0003-1060-058XResearcher IDhttps://www.researcherid.comO-7380-2017Scopus Author IDhttps://www.scopus.com55362894100PersonalCastro, Paula M.L.DSpacehttp://dspace.org/items/f2253992-dc8d-4042-9a0f-597ebcf0a1d6DSpacehttp://dspace.org/items/f2253992-dc8d-4042-9a0f-597ebcf0a1d6CastroPaulaCiência IDhttps://www.ciencia-id.pt7C1F-6C72-354AORCIDhttp://orcid.org0000-0001-8841-6606Researcher IDhttps://www.researcherid.comM-8241-2013Scopus Author IDhttps://www.scopus.com7102781782PersonalHenriques, IsabelDSpacehttp://dspace.org/items/8ff1967b-6347-42bc-aa4e-ff16d1bf4f32DSpacehttp://dspace.org/items/8ff1967b-6347-42bc-aa4e-ff16d1bf4f32HenriquesIsabelCiência IDhttps://www.ciencia-id.ptF516-BFA6-1588ORCIDhttp://orcid.org0000-0001-7717-4939Scopus Author IDhttps://www.scopus.com6701586581HostingInstitutionOrganizationalVeritatie-mailmailto:repositorio@ucp.ptrepositorio@ucp.ptISSNIsPartOf0147-6513DOIIsPartOf10.1016/j.ecoenv.2017.09.0602018-09-13T11:59:03Z20182018-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.14/25685http://purl.org/coar/access_right/c_16ecrestricted accessAerobic granular sludgeMicrobiome analysis454-pyrosequencingPCR-DGGEqPCR1229148 bytesFundação para a Ciência e a TecnologiaSTUDYING THE ROLE OF BACTERIAL COMMUNITIES ON PINE WILT DISEASE DEVELOPMENTCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaFLUOROPHARMA - Biodegradation and removal of chiral and achiral fluorinated pharmaceuticals from wastewaters3599-PPCDTCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ucp.pt/bitstreams/14759388-67a2-4d6d-9d7d-4b9840b25f9e/downloadEcotoxicology and Environmental Safety147905912 |
| spellingShingle | Bacterial community dynamics within an aerobic granular sludge reactor treating wastewater loaded with pharmaceuticals Amorim, Catarina L. Aerobic granular sludge Microbiome analysis 454-pyrosequencing PCR-DGGE qPCR |
| status | SINGLETON |
| subject.fl_str_mv | Aerobic granular sludge Microbiome analysis 454-pyrosequencing PCR-DGGE qPCR |
| title | Bacterial community dynamics within an aerobic granular sludge reactor treating wastewater loaded with pharmaceuticals |
| title_full | Bacterial community dynamics within an aerobic granular sludge reactor treating wastewater loaded with pharmaceuticals |
| title_fullStr | Bacterial community dynamics within an aerobic granular sludge reactor treating wastewater loaded with pharmaceuticals |
| title_full_unstemmed | Bacterial community dynamics within an aerobic granular sludge reactor treating wastewater loaded with pharmaceuticals |
| title_short | Bacterial community dynamics within an aerobic granular sludge reactor treating wastewater loaded with pharmaceuticals |
| title_sort | Bacterial community dynamics within an aerobic granular sludge reactor treating wastewater loaded with pharmaceuticals |
| topic | Aerobic granular sludge Microbiome analysis 454-pyrosequencing PCR-DGGE qPCR |
| topic_facet | Aerobic granular sludge Microbiome analysis 454-pyrosequencing PCR-DGGE qPCR |
| url | http://hdl.handle.net/10400.14/25685 |
| visible | 1 |