Publicação
Bacterial diversity from the source to the tap: a comparative study based on 16S rRNA gene-DGGE and culture-dependent methods
| Resumo: | This study aimed to assess the influence of water treatment and distribution on the bacterial communities with particular emphasis on tap water. Samples from the water treatment plant, the bulk supply distribution system and household taps, supplied by the same drinking water treatment plant, were analyzed using culture-dependent and culture-independent methods. Water treatment imposed alterations in the composition of the bacterial community, although this effect was more evident in the cultivable bacteria rather than among the total community assessed by 16S rRNA gene-denaturing gradient gel electrophoresis (DGGE) profiling. Water disinfection, mainly chlorination, promoted a reduction on bacterial diversity and cultivability, with a shift in the pattern of cultivable bacteria from predominantly Gram-negative to predominately Gram-positive and acid-fast. Downstream of the chlorination stages, tap water, in comparison with raw water, presented higher diversity indices and cultivability percentages. From the source to the tap, members of the Alpha-, Beta- and Gammaproteobacteria were the predominant lineages identified using 16S rRNA gene-DGGE analysis. Although with a lower coverage, the DGGE-based lineage identifications were in agreement with those found using 454-pyrosequencing analysis. Despite the effectiveness of water treatment to eliminate or inactivate most of the bacteria, Proteobacteria such as Acinetobacter, Bosea and Sphingomonadaceae may successfully colonize tap water. |
|---|---|
| Autores principais: | Vaz-Moreira, Ivone |
| Outros Autores: | Egas, Conceição; Nunes, Olga C.; Manaia, Célia M. |
| Assunto: | Bacterial diversity Cultivable bacteria Uncultivable bacteria Drinking water |
| Ano: | 2013 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Universidade Católica Portuguesa |
| Idioma: | inglês |
| Origem: | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| _version_ | 1866895532645941248 |
|---|---|
| author | Vaz-Moreira, Ivone |
| author2 | Egas, Conceição Nunes, Olga C. Manaia, Célia M. |
| author2_role | author author author |
| author_facet | Vaz-Moreira, Ivone Egas, Conceição Nunes, Olga C. Manaia, Célia M. |
| author_role | author |
| contributor_name_str_mv | Veritati |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Vaz-Moreira, Ivone\",\"Person.identifier.orcid\":\"0000-0001-8912-9836\"},{\"Person.name\":\"Egas, Conceição\",\"Person.identifier.orcid\":\"0000-0002-2307-5414\"},{\"Person.name\":\"Nunes, Olga C.\",\"Person.identifier.orcid\":\"0000-0003-4742-2537\"},{\"Person.name\":\"Manaia, Célia M.\",\"Person.identifier.orcid\":\"0000-0002-3674-1789\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Veritati |
| datacite.creators.creator.creatorName.fl_str_mv | Vaz-Moreira, Ivone Egas, Conceição Nunes, Olga C. Manaia, Célia M. |
| datacite.date.Accepted.fl_str_mv | 2013-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2014-05-08T14:08:31Z |
| datacite.date.embargoed.fl_str_mv | 2014-05-08T14:08:31Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Bacterial diversity Cultivable bacteria Uncultivable bacteria Drinking water |
| datacite.titles.title.fl_str_mv | Bacterial diversity from the source to the tap: a comparative study based on 16S rRNA gene-DGGE and culture-dependent methods |
| dc.contributor.none.fl_str_mv | Veritati |
| dc.creator.none.fl_str_mv | Vaz-Moreira, Ivone Egas, Conceição Nunes, Olga C. Manaia, Célia M. |
| dc.date.Accepted.fl_str_mv | 2013-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2014-05-08T14:08:31Z |
| dc.date.embargoed.fl_str_mv | 2014-05-08T14:08:31Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.14/14320 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Oxford University Press |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Bacterial diversity Cultivable bacteria Uncultivable bacteria Drinking water |
| dc.title.fl_str_mv | Bacterial diversity from the source to the tap: a comparative study based on 16S rRNA gene-DGGE and culture-dependent methods |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | This study aimed to assess the influence of water treatment and distribution on the bacterial communities with particular emphasis on tap water. Samples from the water treatment plant, the bulk supply distribution system and household taps, supplied by the same drinking water treatment plant, were analyzed using culture-dependent and culture-independent methods. Water treatment imposed alterations in the composition of the bacterial community, although this effect was more evident in the cultivable bacteria rather than among the total community assessed by 16S rRNA gene-denaturing gradient gel electrophoresis (DGGE) profiling. Water disinfection, mainly chlorination, promoted a reduction on bacterial diversity and cultivability, with a shift in the pattern of cultivable bacteria from predominantly Gram-negative to predominately Gram-positive and acid-fast. Downstream of the chlorination stages, tap water, in comparison with raw water, presented higher diversity indices and cultivability percentages. From the source to the tap, members of the Alpha-, Beta- and Gammaproteobacteria were the predominant lineages identified using 16S rRNA gene-DGGE analysis. Although with a lower coverage, the DGGE-based lineage identifications were in agreement with those found using 454-pyrosequencing analysis. Despite the effectiveness of water treatment to eliminate or inactivate most of the bacteria, Proteobacteria such as Acinetobacter, Bosea and Sphingomonadaceae may successfully colonize tap water. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ucp.pt/bitstreams/479ff27d-c5ec-4660-8bfa-9ecc96146d91/download |
| funding.funder.alternateName_str_mv | FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 6817 - DCRRNI ID |
| id | veritati_c19d98e33e0e2a2f9dbe9105cb367148 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.14/14320 |
| instacron_str | ucp |
| institution | Universidade Católica Portuguesa |
| instname_str | Universidade Católica Portuguesa |
| language | eng |
| network_acronym_str | veritati |
| network_name_str | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| oai_identifier_str | oai:repositorio.ucp.pt:10400.14/14320 |
| organization_str_mv | urn:organizationAcronym:ucp |
| person_str_mv | Vaz-Moreira, Ivone Vaz-Moreira, Ivone https://www.ciencia-id.pt/9E17-544E-800D 9E17-544E-800D http://orcid.org/0000-0001-8912-9836 0000-0001-8912-9836 Egas, Conceição Egas, Conceição http://orcid.org/0000-0002-2307-5414 0000-0002-2307-5414 Nunes, Olga C. Nunes, Olga C. https://www.ciencia-id.pt/BA14-BD30-C9AF BA14-BD30-C9AF http://orcid.org/0000-0003-4742-2537 0000-0003-4742-2537 Manaia, Célia M. Manaia, Célia M. https://www.ciencia-id.pt/C31F-553B-6365 C31F-553B-6365 http://orcid.org/0000-0002-3674-1789 0000-0002-3674-1789 |
| publishDate | 2013 |
| publisher.none.fl_str_mv | Oxford University Press |
| reponame_str | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| repository_id_str | urn:repositoryAcronym:veritati |
| service_str_mv | urn:repositoryAcronym:veritati |
| spelling | engOxford University PressporThis study aimed to assess the influence of water treatment and distribution on the bacterial communities with particular emphasis on tap water. Samples from the water treatment plant, the bulk supply distribution system and household taps, supplied by the same drinking water treatment plant, were analyzed using culture-dependent and culture-independent methods. Water treatment imposed alterations in the composition of the bacterial community, although this effect was more evident in the cultivable bacteria rather than among the total community assessed by 16S rRNA gene-denaturing gradient gel electrophoresis (DGGE) profiling. Water disinfection, mainly chlorination, promoted a reduction on bacterial diversity and cultivability, with a shift in the pattern of cultivable bacteria from predominantly Gram-negative to predominately Gram-positive and acid-fast. Downstream of the chlorination stages, tap water, in comparison with raw water, presented higher diversity indices and cultivability percentages. From the source to the tap, members of the Alpha-, Beta- and Gammaproteobacteria were the predominant lineages identified using 16S rRNA gene-DGGE analysis. Although with a lower coverage, the DGGE-based lineage identifications were in agreement with those found using 454-pyrosequencing analysis. Despite the effectiveness of water treatment to eliminate or inactivate most of the bacteria, Proteobacteria such as Acinetobacter, Bosea and Sphingomonadaceae may successfully colonize tap water.application/pdfporBacterial diversity from the source to the tap: a comparative study based on 16S rRNA gene-DGGE and culture-dependent methodsPersonalVaz-Moreira, IvoneDSpacehttp://dspace.org/items/f3e78f4a-1586-4e5c-a182-81009f20915aDSpacehttp://dspace.org/items/f3e78f4a-1586-4e5c-a182-81009f20915aVaz-MoreiraIvoneCiência IDhttps://www.ciencia-id.pt9E17-544E-800DORCIDhttp://orcid.org0000-0001-8912-9836Scopus Author IDhttps://www.scopus.com16044425800Scopus Author IDhttps://www.scopus.com16044425800PersonalEgas, ConceiçãoDSpacehttp://dspace.org/items/16540431-cad7-493f-aa26-23c28347e494DSpacehttp://dspace.org/items/16540431-cad7-493f-aa26-23c28347e494EgasConceiçãoORCIDhttp://orcid.org0000-0002-2307-5414Researcher IDhttps://www.researcherid.comK-4866-2014PersonalNunes, Olga C.DSpacehttp://dspace.org/items/6b4a1abf-b245-41ad-8335-fa0f379650bbDSpacehttp://dspace.org/items/6b4a1abf-b245-41ad-8335-fa0f379650bbNunesOlgaCiência IDhttps://www.ciencia-id.ptBA14-BD30-C9AFORCIDhttp://orcid.org0000-0003-4742-2537Scopus Author IDhttps://www.scopus.com7005088596PersonalManaia, Célia M.DSpacehttp://dspace.org/items/a5f14577-0aed-4046-97da-5f4216c7aa07DSpacehttp://dspace.org/items/a5f14577-0aed-4046-97da-5f4216c7aa07ManaiaCéliaCiência IDhttps://www.ciencia-id.ptC31F-553B-6365ORCIDhttp://orcid.org0000-0002-3674-1789Scopus Author IDhttps://www.scopus.com6602465318HostingInstitutionOrganizationalVeritatie-mailmailto:repositorio@ucp.ptrepositorio@ucp.ptISSNIsPartOf0168-6496DOIIsPartOf10.1111/1574-6941.120022014-05-08T14:08:31Z20132013-01-01T00:00:00ZHandlehttp://hdl.handle.net/10400.14/14320http://purl.org/coar/access_right/c_16ecrestricted accessBacterial diversityCultivable bacteriaUncultivable bacteriaDrinking water798423 bytesFundação para a Ciência e a TecnologiaStrategic Project - LA 16 - 2011-20126817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://repositorio.ucp.pt/bitstreams/479ff27d-c5ec-4660-8bfa-9ecc96146d91/downloadFEMS Microbiology Ecology832361374 |
| spellingShingle | Bacterial diversity from the source to the tap: a comparative study based on 16S rRNA gene-DGGE and culture-dependent methods Vaz-Moreira, Ivone Bacterial diversity Cultivable bacteria Uncultivable bacteria Drinking water |
| status | SINGLETON |
| subject.fl_str_mv | Bacterial diversity Cultivable bacteria Uncultivable bacteria Drinking water |
| title | Bacterial diversity from the source to the tap: a comparative study based on 16S rRNA gene-DGGE and culture-dependent methods |
| title_full | Bacterial diversity from the source to the tap: a comparative study based on 16S rRNA gene-DGGE and culture-dependent methods |
| title_fullStr | Bacterial diversity from the source to the tap: a comparative study based on 16S rRNA gene-DGGE and culture-dependent methods |
| title_full_unstemmed | Bacterial diversity from the source to the tap: a comparative study based on 16S rRNA gene-DGGE and culture-dependent methods |
| title_short | Bacterial diversity from the source to the tap: a comparative study based on 16S rRNA gene-DGGE and culture-dependent methods |
| title_sort | Bacterial diversity from the source to the tap: a comparative study based on 16S rRNA gene-DGGE and culture-dependent methods |
| topic | Bacterial diversity Cultivable bacteria Uncultivable bacteria Drinking water |
| topic_facet | Bacterial diversity Cultivable bacteria Uncultivable bacteria Drinking water |
| url | http://hdl.handle.net/10400.14/14320 |
| visible | 1 |