Publicação
Genome sequencing reveals a phage in Helicobacter pylori
| Resumo: | Helicobacter pylori chronically infects the gastric mucosa in more than half of the human population; in a subset of this population, its presence is associated with development of severe disease, such as gastric cancer. Genomic analysis of several strains has revealed an extensive H pylori pan-genome, likely to grow as more genomes are sampled. Here we describe the draft genome sequence (63 contigs; 26× mean coverage) of H pylori strain B45, isolated from a patient with gastric mucosa-associated lymphoid tissue (MALT) lymphoma. The major finding was a 24.6-kb prophage integrated in the bacterial genome. The prophage shares most of its genes (22/27) with prophage region II of Helicobacter acinonychis strain Sheeba. After UV treatment of liquid cultures, circular DNA carrying the prophage integrase gene could be detected, and intracellular tailed phage-like particles were observed in H pylori cells by transmission electron microscopy, indicating that phage production can be induced from the prophage. PCR amplification and sequencing of the integrase gene from 341 H pylori strains from different geographic regions revealed a high prevalence of the prophage (21.4%). Phylogenetic reconstruction showed four distinct clusters in the integrase gene, three of which tended to be specific for geographic regions. Our study implies that phages may play important roles in the ecology and evolution of H pylori. |
|---|---|
| Autores principais: | Lehours, Philippe |
| Outros Autores: | Vale, Filipa F.; Bjursell, Magnus K.; Melefors, Ojar; Advani, Reza; Glavas, Steve; Guegueniat, Julia; Gontier, Etienne; Lacomme, Sabrina; Matos, António Alves; Menard, Armelle; Mégraud, Francis; Engstrand, Lars; Andersson, Anders F. |
| Ano: | 2011 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Universidade Católica Portuguesa |
| Idioma: | inglês |
| Origem: | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| _version_ | 1868984615410073600 |
|---|---|
| author | Lehours, Philippe |
| author2 | Vale, Filipa F. Bjursell, Magnus K. Melefors, Ojar Advani, Reza Glavas, Steve Guegueniat, Julia Gontier, Etienne Lacomme, Sabrina Matos, António Alves Menard, Armelle Mégraud, Francis Engstrand, Lars Andersson, Anders F. |
| author2_role | author author author author author author author author author author author author author |
| author_facet | Lehours, Philippe Vale, Filipa F. Bjursell, Magnus K. Melefors, Ojar Advani, Reza Glavas, Steve Guegueniat, Julia Gontier, Etienne Lacomme, Sabrina Matos, António Alves Menard, Armelle Mégraud, Francis Engstrand, Lars Andersson, Anders F. |
| author_role | author |
| contributor_name_str_mv | Veritati |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Lehours, Philippe\"},{\"Person.name\":\"Vale, Filipa F.\"},{\"Person.name\":\"Bjursell, Magnus K.\"},{\"Person.name\":\"Melefors, Ojar\"},{\"Person.name\":\"Advani, Reza\"},{\"Person.name\":\"Glavas, Steve\"},{\"Person.name\":\"Guegueniat, Julia\"},{\"Person.name\":\"Gontier, Etienne\"},{\"Person.name\":\"Lacomme, Sabrina\"},{\"Person.name\":\"Matos, António Alves\"},{\"Person.name\":\"Menard, Armelle\"},{\"Person.name\":\"Mégraud, Francis\"},{\"Person.name\":\"Engstrand, Lars\"},{\"Person.name\":\"Andersson, Anders F.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Veritati |
| datacite.creators.creator.creatorName.fl_str_mv | Lehours, Philippe Vale, Filipa F. Bjursell, Magnus K. Melefors, Ojar Advani, Reza Glavas, Steve Guegueniat, Julia Gontier, Etienne Lacomme, Sabrina Matos, António Alves Menard, Armelle Mégraud, Francis Engstrand, Lars Andersson, Anders F. |
| datacite.date.Accepted.fl_str_mv | 2011-11-15T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-08-09T08:36:27Z |
| datacite.date.embargoed.fl_str_mv | 2023-08-09T08:36:27Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.titles.title.fl_str_mv | Genome sequencing reveals a phage in Helicobacter pylori |
| dc.contributor.none.fl_str_mv | Veritati |
| dc.creator.none.fl_str_mv | Lehours, Philippe Vale, Filipa F. Bjursell, Magnus K. Melefors, Ojar Advani, Reza Glavas, Steve Guegueniat, Julia Gontier, Etienne Lacomme, Sabrina Matos, António Alves Menard, Armelle Mégraud, Francis Engstrand, Lars Andersson, Anders F. |
| dc.date.Accepted.fl_str_mv | 2011-11-15T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-08-09T08:36:27Z |
| dc.date.embargoed.fl_str_mv | 2023-08-09T08:36:27Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.14/42051 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by-nc-sa/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.title.fl_str_mv | Genome sequencing reveals a phage in Helicobacter pylori |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Helicobacter pylori chronically infects the gastric mucosa in more than half of the human population; in a subset of this population, its presence is associated with development of severe disease, such as gastric cancer. Genomic analysis of several strains has revealed an extensive H pylori pan-genome, likely to grow as more genomes are sampled. Here we describe the draft genome sequence (63 contigs; 26× mean coverage) of H pylori strain B45, isolated from a patient with gastric mucosa-associated lymphoid tissue (MALT) lymphoma. The major finding was a 24.6-kb prophage integrated in the bacterial genome. The prophage shares most of its genes (22/27) with prophage region II of Helicobacter acinonychis strain Sheeba. After UV treatment of liquid cultures, circular DNA carrying the prophage integrase gene could be detected, and intracellular tailed phage-like particles were observed in H pylori cells by transmission electron microscopy, indicating that phage production can be induced from the prophage. PCR amplification and sequencing of the integrase gene from 341 H pylori strains from different geographic regions revealed a high prevalence of the prophage (21.4%). Phylogenetic reconstruction showed four distinct clusters in the integrase gene, three of which tended to be specific for geographic regions. Our study implies that phages may play important roles in the ecology and evolution of H pylori. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10400.14/42051 |
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| language | eng |
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| oai_identifier_str | oai:repositorio.ucp.pt:10400.14/42051 |
| organization_str_mv | urn:organizationAcronym:ucp |
| person_str_mv | Lehours, Philippe Vale, Filipa F. Bjursell, Magnus K. Melefors, Ojar Advani, Reza Glavas, Steve Guegueniat, Julia Gontier, Etienne Lacomme, Sabrina Matos, António Alves Menard, Armelle Mégraud, Francis Engstrand, Lars Andersson, Anders F. |
| publishDate | 2011 |
| repo_facet_str | urn:repositoryAcronym:veritati{{{_:::_}}}Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| reponame_str | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
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| spelling | engpt_PTHelicobacter pylori chronically infects the gastric mucosa in more than half of the human population; in a subset of this population, its presence is associated with development of severe disease, such as gastric cancer. Genomic analysis of several strains has revealed an extensive H pylori pan-genome, likely to grow as more genomes are sampled. Here we describe the draft genome sequence (63 contigs; 26× mean coverage) of H pylori strain B45, isolated from a patient with gastric mucosa-associated lymphoid tissue (MALT) lymphoma. The major finding was a 24.6-kb prophage integrated in the bacterial genome. The prophage shares most of its genes (22/27) with prophage region II of Helicobacter acinonychis strain Sheeba. After UV treatment of liquid cultures, circular DNA carrying the prophage integrase gene could be detected, and intracellular tailed phage-like particles were observed in H pylori cells by transmission electron microscopy, indicating that phage production can be induced from the prophage. PCR amplification and sequencing of the integrase gene from 341 H pylori strains from different geographic regions revealed a high prevalence of the prophage (21.4%). Phylogenetic reconstruction showed four distinct clusters in the integrase gene, three of which tended to be specific for geographic regions. Our study implies that phages may play important roles in the ecology and evolution of H pylori.application/pdfpt_PTGenome sequencing reveals a phage in Helicobacter pyloriLehours, PhilippeVale, Filipa F.Bjursell, Magnus K.Melefors, OjarAdvani, RezaGlavas, SteveGuegueniat, JuliaGontier, EtienneLacomme, SabrinaMatos, António AlvesMenard, ArmelleMégraud, FrancisEngstrand, LarsAndersson, Anders F.HostingInstitutionOrganizationalVeritatie-mailmailto:repositorio@ucp.ptrepositorio@ucp.ptISSNIsPartOf2161-2129DOIIsPartOf10.1128/mBio.00239-112023-08-09T08:36:27Z2011-11-152011-11-15T00:00:00ZHandlehttp://hdl.handle.net/10400.14/42051http://purl.org/coar/access_right/c_abf2open access1075305 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2011-11-15http://creativecommons.org/licenses/by-nc-sa/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ucp.pt/bitstreams/c906afb2-b085-4374-b12f-91835f6921f0/downloadmBio26 |
| spellingShingle | Genome sequencing reveals a phage in Helicobacter pylori Lehours, Philippe |
| status | SINGLETON |
| title | Genome sequencing reveals a phage in Helicobacter pylori |
| title_full | Genome sequencing reveals a phage in Helicobacter pylori |
| title_fullStr | Genome sequencing reveals a phage in Helicobacter pylori |
| title_full_unstemmed | Genome sequencing reveals a phage in Helicobacter pylori |
| title_short | Genome sequencing reveals a phage in Helicobacter pylori |
| title_sort | Genome sequencing reveals a phage in Helicobacter pylori |
| url | http://hdl.handle.net/10400.14/42051 |
| visible | 1 |