Publication
Nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata link
| Summary: | Bauhinia forficata Link is a plant rich in polyphenols that has been used mainly for its hypoglycemic activity, which is related to its antioxidant and anti-inflammatory potential. However, the beneficial effect of these bioactive compounds is directly dependent on their bioaccessibility and bioavailability, requiring processing techniques that can improve and preserve their biological activities. This work aimed to obtain nanocapsulated extracts from the infusion (ESIN) and decoction (ESDC) of B. forficata Link leaves, by spray drying. The encapsulating agents used were maltodextrin and colloidal silicon dioxide. The nanocapsules were characterized by HPLC-PDA-ESI-IT-MSn, evaluated the bioaccessibility of polyphenols after simulated digestion and their antioxidant activity. Additionally, an extensive physicochemical characterization of the nanocapsulated extracts was carried out and their stability and technological parameters were evaluated. The ESIN and ESDC extracts had yields of 57.3 % and 62.7 %, with average nanocapsules sizes of 0.202 μm and 0.179 μm, low humidity and water activity (<0.5), powder density and proper flow properties (Hausner ratio ≤ 1.25; Carr index 18–19 %). Scanning electron microscopy showed a spherical and amorphous morphology and low viscosity, which may have favored the solubility profile. The phenolic compounds of the nanocapsules degraded after 400 °C, showing high thermal stability. The infrared spectra identified the presence of maltodextrin and phenolic compounds and that there were no reactions between them. Chromatography confirmed the presence of phenolic compounds, mainly flavonols and their O-glycosylated derivatives, as well as carbohydrates, probably maltodextrin. Simulated in vitro digestion showed that polyphenols and flavonoids from ESIN and ESDC nanocapsules were bioaccessible after the gastric phase (49.38 % and 64.17 % of polyphenols and 64.08 % and 36.61 % of flavonoids) and duodenal (52.68 % and 79.06 % of polyphenols and 13.24 % and 139.03 % of flavoids), with a variation from 52.27 % to 70.55 % of the antioxidant activity maintained, by the ORAC method, after gastric digestion and still 25 %, after duodenal. Therefore, the nanoencapsulation of extracts of B. forficata is a viable option for the preservation of their bioactive compounds, making them bioaccessible and with antioxidant activity, which make them suitable for incorporation into various nutraceutical formulations, such as capsules, tablets and sachets. |
|---|---|
| Main Authors: | Souza, Bárbara Verônica Cardoso de |
| Other Authors: | Sousa, Mariana de Morais; Sattler, José Augusto Gasparotto; Santana, Ana Cristina Sousa Gramoza Vilarinho; Carvalho, Rusbene Bruno Fonseca de; Neto, José de Sousa Lima; Borges, Fernando de Matos; Numa, Iramaia Angelica Neri; Ribeiro, Alessandra Braga; Nunes, Lívio César Cunha |
| Subject: | Bioactive compounds ORAC Phenolic compounds Simulated digestion Spray drying |
| Year: | 2022 |
| Country: | Portugal |
| Document type: | article |
| Access type: | open access |
| Associated institution: | Universidade Católica Portuguesa |
| Language: | English |
| Origin: | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| _version_ | 1866895479957094400 |
|---|---|
| author | Souza, Bárbara Verônica Cardoso de |
| author2 | Sousa, Mariana de Morais Sattler, José Augusto Gasparotto Santana, Ana Cristina Sousa Gramoza Vilarinho Carvalho, Rusbene Bruno Fonseca de Neto, José de Sousa Lima Borges, Fernando de Matos Numa, Iramaia Angelica Neri Ribeiro, Alessandra Braga Nunes, Lívio César Cunha |
| author2_role | author author author author author author author author author |
| author_facet | Souza, Bárbara Verônica Cardoso de Sousa, Mariana de Morais Sattler, José Augusto Gasparotto Santana, Ana Cristina Sousa Gramoza Vilarinho Carvalho, Rusbene Bruno Fonseca de Neto, José de Sousa Lima Borges, Fernando de Matos Numa, Iramaia Angelica Neri Ribeiro, Alessandra Braga Nunes, Lívio César Cunha |
| author_role | author |
| contributor_name_str_mv | Veritati |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Souza, Bárbara Verônica Cardoso de\"},{\"Person.name\":\"Sousa, Mariana de Morais\"},{\"Person.name\":\"Sattler, José Augusto Gasparotto\"},{\"Person.name\":\"Santana, Ana Cristina Sousa Gramoza Vilarinho\"},{\"Person.name\":\"Carvalho, Rusbene Bruno Fonseca de\"},{\"Person.name\":\"Neto, José de Sousa Lima\"},{\"Person.name\":\"Borges, Fernando de Matos\"},{\"Person.name\":\"Numa, Iramaia Angelica Neri\"},{\"Person.name\":\"Ribeiro, Alessandra Braga\"},{\"Person.name\":\"Nunes, Lívio César Cunha\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Veritati |
| datacite.creators.creator.creatorName.fl_str_mv | Souza, Bárbara Verônica Cardoso de Sousa, Mariana de Morais Sattler, José Augusto Gasparotto Santana, Ana Cristina Sousa Gramoza Vilarinho Carvalho, Rusbene Bruno Fonseca de Neto, José de Sousa Lima Borges, Fernando de Matos Numa, Iramaia Angelica Neri Ribeiro, Alessandra Braga Nunes, Lívio César Cunha |
| datacite.date.Accepted.fl_str_mv | 2022-12-30T00:00:00Z |
| datacite.date.available.fl_str_mv | 2022-11-30T15:15:35Z |
| datacite.date.embargoed.fl_str_mv | 2022-11-30T15:15:35Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Bioactive compounds ORAC Phenolic compounds Simulated digestion Spray drying |
| datacite.titles.title.fl_str_mv | Nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata link |
| dc.contributor.none.fl_str_mv | Veritati |
| dc.creator.none.fl_str_mv | Souza, Bárbara Verônica Cardoso de Sousa, Mariana de Morais Sattler, José Augusto Gasparotto Santana, Ana Cristina Sousa Gramoza Vilarinho Carvalho, Rusbene Bruno Fonseca de Neto, José de Sousa Lima Borges, Fernando de Matos Numa, Iramaia Angelica Neri Ribeiro, Alessandra Braga Nunes, Lívio César Cunha |
| dc.date.Accepted.fl_str_mv | 2022-12-30T00:00:00Z |
| dc.date.available.fl_str_mv | 2022-11-30T15:15:35Z |
| dc.date.embargoed.fl_str_mv | 2022-11-30T15:15:35Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10400.14/39457 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Bioactive compounds ORAC Phenolic compounds Simulated digestion Spray drying |
| dc.title.fl_str_mv | Nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata link |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Bauhinia forficata Link is a plant rich in polyphenols that has been used mainly for its hypoglycemic activity, which is related to its antioxidant and anti-inflammatory potential. However, the beneficial effect of these bioactive compounds is directly dependent on their bioaccessibility and bioavailability, requiring processing techniques that can improve and preserve their biological activities. This work aimed to obtain nanocapsulated extracts from the infusion (ESIN) and decoction (ESDC) of B. forficata Link leaves, by spray drying. The encapsulating agents used were maltodextrin and colloidal silicon dioxide. The nanocapsules were characterized by HPLC-PDA-ESI-IT-MSn, evaluated the bioaccessibility of polyphenols after simulated digestion and their antioxidant activity. Additionally, an extensive physicochemical characterization of the nanocapsulated extracts was carried out and their stability and technological parameters were evaluated. The ESIN and ESDC extracts had yields of 57.3 % and 62.7 %, with average nanocapsules sizes of 0.202 μm and 0.179 μm, low humidity and water activity (<0.5), powder density and proper flow properties (Hausner ratio ≤ 1.25; Carr index 18–19 %). Scanning electron microscopy showed a spherical and amorphous morphology and low viscosity, which may have favored the solubility profile. The phenolic compounds of the nanocapsules degraded after 400 °C, showing high thermal stability. The infrared spectra identified the presence of maltodextrin and phenolic compounds and that there were no reactions between them. Chromatography confirmed the presence of phenolic compounds, mainly flavonols and their O-glycosylated derivatives, as well as carbohydrates, probably maltodextrin. Simulated in vitro digestion showed that polyphenols and flavonoids from ESIN and ESDC nanocapsules were bioaccessible after the gastric phase (49.38 % and 64.17 % of polyphenols and 64.08 % and 36.61 % of flavonoids) and duodenal (52.68 % and 79.06 % of polyphenols and 13.24 % and 139.03 % of flavoids), with a variation from 52.27 % to 70.55 % of the antioxidant activity maintained, by the ORAC method, after gastric digestion and still 25 %, after duodenal. Therefore, the nanoencapsulation of extracts of B. forficata is a viable option for the preservation of their bioactive compounds, making them bioaccessible and with antioxidant activity, which make them suitable for incorporation into various nutraceutical formulations, such as capsules, tablets and sachets. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://repositorio.ucp.pt/bitstreams/6e37e7ff-93f0-4db9-9ba8-f7051d8629f8/download |
| id | veritati_f8d4a3d0aa104fcdff152dc01ec33168 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10400.14/39457 |
| instacron_str | ucp |
| institution | Universidade Católica Portuguesa |
| instname_str | Universidade Católica Portuguesa |
| language | eng |
| network_acronym_str | veritati |
| network_name_str | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| oai_identifier_str | oai:repositorio.ucp.pt:10400.14/39457 |
| organization_str_mv | urn:organizationAcronym:ucp |
| person_str_mv | Souza, Bárbara Verônica Cardoso de Sousa, Mariana de Morais Sattler, José Augusto Gasparotto Santana, Ana Cristina Sousa Gramoza Vilarinho Carvalho, Rusbene Bruno Fonseca de Neto, José de Sousa Lima Borges, Fernando de Matos Numa, Iramaia Angelica Neri Ribeiro, Alessandra Braga Nunes, Lívio César Cunha |
| publishDate | 2022 |
| reponame_str | Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| repository_id_str | urn:repositoryAcronym:veritati |
| service_str_mv | urn:repositoryAcronym:veritati |
| spelling | engpt_PTBauhinia forficata Link is a plant rich in polyphenols that has been used mainly for its hypoglycemic activity, which is related to its antioxidant and anti-inflammatory potential. However, the beneficial effect of these bioactive compounds is directly dependent on their bioaccessibility and bioavailability, requiring processing techniques that can improve and preserve their biological activities. This work aimed to obtain nanocapsulated extracts from the infusion (ESIN) and decoction (ESDC) of B. forficata Link leaves, by spray drying. The encapsulating agents used were maltodextrin and colloidal silicon dioxide. The nanocapsules were characterized by HPLC-PDA-ESI-IT-MSn, evaluated the bioaccessibility of polyphenols after simulated digestion and their antioxidant activity. Additionally, an extensive physicochemical characterization of the nanocapsulated extracts was carried out and their stability and technological parameters were evaluated. The ESIN and ESDC extracts had yields of 57.3 % and 62.7 %, with average nanocapsules sizes of 0.202 μm and 0.179 μm, low humidity and water activity (<0.5), powder density and proper flow properties (Hausner ratio ≤ 1.25; Carr index 18–19 %). Scanning electron microscopy showed a spherical and amorphous morphology and low viscosity, which may have favored the solubility profile. The phenolic compounds of the nanocapsules degraded after 400 °C, showing high thermal stability. The infrared spectra identified the presence of maltodextrin and phenolic compounds and that there were no reactions between them. Chromatography confirmed the presence of phenolic compounds, mainly flavonols and their O-glycosylated derivatives, as well as carbohydrates, probably maltodextrin. Simulated in vitro digestion showed that polyphenols and flavonoids from ESIN and ESDC nanocapsules were bioaccessible after the gastric phase (49.38 % and 64.17 % of polyphenols and 64.08 % and 36.61 % of flavonoids) and duodenal (52.68 % and 79.06 % of polyphenols and 13.24 % and 139.03 % of flavoids), with a variation from 52.27 % to 70.55 % of the antioxidant activity maintained, by the ORAC method, after gastric digestion and still 25 %, after duodenal. Therefore, the nanoencapsulation of extracts of B. forficata is a viable option for the preservation of their bioactive compounds, making them bioaccessible and with antioxidant activity, which make them suitable for incorporation into various nutraceutical formulations, such as capsules, tablets and sachets.application/pdfpt_PTNanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata linkSouza, Bárbara Verônica Cardoso deSousa, Mariana de MoraisSattler, José Augusto GasparottoSantana, Ana Cristina Sousa Gramoza VilarinhoCarvalho, Rusbene Bruno Fonseca deNeto, José de Sousa LimaBorges, Fernando de MatosNuma, Iramaia Angelica NeriRibeiro, Alessandra BragaNunes, Lívio César CunhaHostingInstitutionOrganizationalVeritatie-mailmailto:repositorio@ucp.ptrepositorio@ucp.ptISSNIsPartOf2666-5662DOIIsPartOf10.1016/j.fochms.2022.1001442022-11-30T15:15:35Z2022-12-302022-12-30T00:00:00ZHandlehttp://hdl.handle.net/10400.14/39457http://purl.org/coar/access_right/c_abf2open accessBioactive compoundsORACPhenolic compoundsSimulated digestionSpray drying2052359 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2022-12-30http://creativecommons.org/licenses/by-nc-nd/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://repositorio.ucp.pt/bitstreams/6e37e7ff-93f0-4db9-9ba8-f7051d8629f8/downloadFood Chemistry: Molecular Sciences5 |
| spellingShingle | Nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata link Souza, Bárbara Verônica Cardoso de Bioactive compounds ORAC Phenolic compounds Simulated digestion Spray drying |
| status | SINGLETON |
| subject.fl_str_mv | Bioactive compounds ORAC Phenolic compounds Simulated digestion Spray drying |
| title | Nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata link |
| title_full | Nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata link |
| title_fullStr | Nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata link |
| title_full_unstemmed | Nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata link |
| title_short | Nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata link |
| title_sort | Nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata link |
| topic | Bioactive compounds ORAC Phenolic compounds Simulated digestion Spray drying |
| topic_facet | Bioactive compounds ORAC Phenolic compounds Simulated digestion Spray drying |
| url | http://hdl.handle.net/10400.14/39457 |
| visible | 1 |