Author(s):
Añibarro-Ortega, Mikel ; López, Víctor ; Núñez, Sonia ; Petrović, Jovana ; Mandim, Filipa ; Barros, Lillian ; Soković, Marina ; Ferreira, Isabel C.F.R. ; Dias, Maria Inês ; Pinela, José
Date: 2024
Persistent ID: http://hdl.handle.net/10198/29884
Origin: Biblioteca Digital do IPB
Project/scholarship:
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT;
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT;
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT;
info:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F146614%2F2019/PT;
info:eu-repo/grantAgreement/FCT/CEEC INST 2018/CEECINST%2F00016%2F2018%2FCP1505%2FCT0004/PT;
info:eu-repo/grantAgreement/FCT/CEEC IND 2018/CEECIND%2F01011%2F2018%2FCP1578%2FCT0002/PT;
Subject(s): Phenolic compounds; Antioxidant activity; Antidiabetic activity; Cytotoxic activity; By-product valorization; Functional food ingredients
Description
The phenolic composition and bioactive properties of Holland and Italian bell pepper plant (Capsicum annuum L.) hydroethanolic extracts were investigated by HPLC-DAD-ESI/MS and in vitro cell- and enzyme-based assays, respectively. O-Glycosylated luteolin derivatives were identified as the major compounds, representing -60% of the phenolic fraction. Both extracts displayed remarkable antioxidant activity and ability to inhibit alpha-glucosidase and the formation of advanced glycation end products (AGEs) compared to the positive controls, which yielded statistically higher or equal IC50 values. Notably, the extracts were up to 57 times more effective than aminoguanidine in inhibiting AGEs. The lipase enzyme involved in fat metabolism and the pro-inflammatory mediator nitric oxide were also inhibited in some extent, with orlistat and dexamethasone being 10-13.4-fold and -17fold more effective than the extracts, respectively. A notable cytotoxicity occurred on the tested human tumor cell lines, mainly on Caco-2 with GI50 values ranging from 18 to 65 mu g/mL. Furthermore, activity greater than that of food preservatives was observed against some foodborne fungi and bacteria. These results highlight bell pepper plant by-products as a renewable source of functional ingredients that could be utilized in food, nutraceutical, and medicinal formulations to manage type 2 diabetes, obesity, and other oxidative stress-mediated conditions.
CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2020) received financial support from the Foundation for Science and Technology (FCT, Portugal) through national funds FCT/MCTES (PIDDAC). National funding by FCT, through the individual research grants of M. Anibarro-Ortega (10.54499/2020.06297.BD) and F. Mandim (SFRH/BD/146614/2019) , and through the scientific employment program -contract with M.I. Dias (10.54499/CEECINST/00016/2018/CP1505/CT0004) , L. Barros (CEEC-INST) , and J. Pinela (10.54499/CEECIND/01011/2018/CP1578/CT0002) . J. Petrovic and M. Sokovic are grateful to the Serbian Ministry of Education, Science and Technological Development (contract nos. 451-03-9/2021-14/200007) . V. L opez is grateful to the San Jorge University for the financial support through Proyecto Interno ID 2223025 and to the Government of Aragon for financial support of the Phyto-Pharm group (ref. B44_20R) .
