Autor(es):
Pascoalino, Liege ; Finimundy, Tiane C. ; Pires, Tânia C.S.P. ; Pereira, Carla ; Barros, Lillian ; Ferreira, Isabel C.F.R. ; Oliveira, Beatriz ; Barreira, João C.M. ; Reis, Filipa S.
Data: 2025
Identificador Persistente: http://hdl.handle.net/10198/30930
Origem: Biblioteca Digital do IPB
Projeto/bolsa:
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT;
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT;
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT;
info:eu-repo/grantAgreement/FCT/CEEC IND4ed/2021.03728.CEECIND%2FCP1677%2FCT0001/PT;
info:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F04479%2F2017%2FCP1403%2FCT0002/PT;
info:eu-repo/grantAgreement/FCT/OE/UI%2FBD%2F153745%2F2022/PT;
Assunto(s): Apple pomace; Bio-residues; Bioactivity; Circular economy; Functional foods; Nutraceuticals
Descrição
This study investigates bioactive compounds in apple pomace (AP) obtained from Malus domestica varieties Granny Smith and Cripps Pink, emphasizing its potential for developing high-added-value products from industrial surplus. Regarding its chemical characterization, AP stood out for its high concentration of dietary fibers (7.1g/100g fw), polyunsaturated fatty acids (linoleic and oleic acids), α-tocopherol (0.64 mg/100g fw), and phenolic compounds. Regarding these last, the profile obtained by HPLC-DAD-ESI/MS allowed the identification of 23 phenolic compounds, with higher concentrations of quercetin and flavonoids in pomace than whole apples. Moreover, concerning evaluating AP bioactivity through in vitro methods, the analyzed AP samples, with seeds and stems (APSS) and without (APA), exhibited enhanced antioxidant activity, particularly in the OxHLIA test, preserving erythrocyte integrity. Additionally, the pomace demonstrated significant antibacterial activity against Yersinia enterocolitica, Staphylococcus aureus, and Escherichia coli, with a minimum inhibitory concentration of 5 mg/mL. Finally, no cytotoxic or anti-inflammatory effects were observed, as GI50 values exceeded 400 μg/mL (maximum tested concentration). These findings highlight apple pomace as a rich source of bioactive compounds, promoting its valorization in food safety and preservation while encouraging sustainable practices utilizing industrial bio-residues.
This work was supported by national funds through FCT/MCTES (PIDDAC): CIMO, UIDB/00690/2020 (DOI: 10.54499/UIDB/00690/ 2020) and UIDP/00690/2020 (DOI: 10.54499/UIDP/00690/2020); SusTEC, LA/P/0007/2020 (DOI:10.54499/LA/P/0007/2020). The authors are grateful to FEDER Cooperación Interreg VI A Espanha – Portugal (POCTEP) 2021–2027 for financial support through the project TRANSCoLAB PLUS 0112_TRANSCOLAB_PLUS_2_P. The authors also thank the National funding by FCT, through the institutional scientific employment program contract with L. Barros, and the individual scientific employment program contract with F.S. Reis (2021.03728.CEECIND) and J.C.M. Barreira (CEECIND/04479/2017). L.A. Pascoalino thanks FCT for her research grant (UI/BD/153745/2022).
