Author(s): Castilho, Paula C. ; Costa, Maria do Céu ; Rodrigues, Ana ; Branco, Pedro Costa ; Costa, Mafalaa
Date: 2004
Persistent ID: http://hdl.handle.net/10400.13/3271
Origin: DigitUMa - Repositório da Universidade da Madeira
Subject(s): APCI-MS; HPLC; Madeira laurel oil; Triacylglycerols; .; Faculdade de Ciências Exatas e da Engenharia
Madeira laurel oil was fractionated by liquid ex traction combined with TLC, and TAGs were analyzed by HPLC coupled with atmospheric pressure chemical ionization-MS (APCI-MS). Eluted molecular species compositions of the eluted TAG in the complex natural mixture were determined by GC identification of FAME and by LC-atmospheric pressure chemi cal ionization (APCI)-MS analysis of the lipid. The APCI-MS spectra of most TAG exhibited [M + H]+ and [M − RCOO]+ ions, which defined the M.W. and the molecular association of fatty acyl residues, respectively. Despite the relatively high de gree of saturation, with a saturated/unsaturated ratio of 0.70, no totally saturated TAG nor mixed asymmetric TAG with two sat urated FA (SSM or SSU, where S is saturated, M is monounsatu rated, and U is unsaturated) were found. This type of molecular structure provides a possible explanation for the relatively low m.p. (12–15°C) and also the high oxidative resistance observed.
