Author(s): Rodrigues, Artur Filipe ; Tavares, Ana P. M. ; Simões, Susana ; Silva, Rui P. F. F. ; Sobrino, Tomás ; Figueiredo, Bruno R. ; Sales, Goreti ; Ferreira, Lino
Date: 2023
Persistent ID: https://hdl.handle.net/10316/105375
Origin: Estudo Geral - Universidade de Coimbra
Project/scholarship: info:eu-repo/grantAgreement/EC/H2020/101003413/EU;
Subject(s): Neuromodulation; graphene; near-infrared radiation; optoelectronic; tissue regeneration
Graphene-based materials (GBM) have been investigated in recent years with the aim of developing flexible interfaces to address a range of neurological disorders, where electrical stimulation may improve brain function and tissue regeneration. The recent discovery that GBM electrodes can generate an electrical response upon light exposure has inspired the development of non-genetic approaches capable of selectively modulate brain cells without genetic manipulation (i.e., optogenetics). Here, we propose the conjugation of graphene with upconversion nanoparticles (UCNP), which enable wireless transcranial activation using tissuepenetrating near-infrared (NIR) radiation. Following a Design of Experiments approach, we first investigated the influence of different host matrices and dopants commonly used to synthesize UCNPs in the electrical response of graphene. Two UCNP formulations achieving optimal enhancement of electric conductivity upon NIR activation at λ = 780 or 980 nm were identified. These formulations were then covalently attached to graphene nanoplatelets following selective hydroxyl derivatization. The resulting nanocomposites were evaluated in vitro using SH-SY5Y human neuroblastoma cells. NIR activation at λ = 980 nm promoted cell proliferation and downregulated neuronal and glial differentiation markers, suggesting the potential application of GBMs in minimally invasive stimulation of cells for tissue regeneration.
D813-6344-58E8 | Artur Filipe Cardoso Duarte Rodrigues
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