Author(s):
Carrapita, Jorge ; Abrantes, Ana Margarida ; Campelos, Sofia ; Gonçalves, Ana Cristina ; Cardoso, Dulce ; Ribeiro, Ana Bela Sarmento ; Rocha, Clara ; Santos, Jorge Nunes ; Botelho, Maria Filomena ; Tralhão, José Guilherme ; Farges, Olivier ; Barbosa, Jorge Maciel
Date: 2016
Persistent ID: https://hdl.handle.net/10316/108917
Origin: Estudo Geral - Universidade de Coimbra
Project/scholarship:
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID/PT;
Subject(s): Animals; Apoptosis; Cell Survival; Hepatectomy; Hepatocytes; Ligation; Liver; Liver Function Tests; Liver Regeneration; Male; Membrane Potential, Mitochondrial; Necrosis; Oxidative Stress; Primary Cell Culture; Rats; Rats, Wistar; Spleen; Splenic Artery; Superoxides; Survival Analysis
Description
It was reported that prevention of acute portal overpressure in small-for-size livers by inflow modulation results in a better postoperative outcome. The aim is to investigate the impact of portal blood flow reduction by splenic artery ligation after major hepatectomy in a murine model. Forty-eight rats were subjected to an 85% hepatectomy or 85% hepatectomy and splenic artery ligation. Both groups were evaluated at 24, 48, 72 and 120 post-operative hours: liver function, regeneration and viability. All methods and experiments were carried out in accordance with Coimbra University guidelines. Splenic artery ligation produces viability increase after 24 h, induces a relative decrease in oxidative stress during the first 48 hours, allows antioxidant capacity increment after 24 h, which is reflected in a decrease of half-time normalized liver curve at 48 h and at 72 h and in an increase of mitotic index between 48 h and 72 h. Splenic artery ligation combined with 85% hepatectomy in a murine model, allows portal inflow modulation, promoting an increase in hepatocellular viability and regeneration, without impairing the function, probably by inducing a less marked elevation of oxidative stress at first 48 hours.
