Author(s):
Sato, Camila Massae ; Sanchez, Maria Carmen Arroyo ; Celeste, Beatriz Julieta ; Duthie, Malcolm S ; Guderian, Jeffrey ; Reed, Steven G ; Brito, Maria Edileuza Felinto de ; Campos, Marliane Batista ; Encarna??o, Helia Valeria de Souza ; Guerra, Jorge ; Mesquita, Tirza Gabrielle Ramos de ; Pinheiro, Suzana Kanawati ; Ramasawmy, Rajendranath ; Silveira, Fernando Tobias ; Souza, Marina de Assis ; Goto, Hiro
Date: 2017
Origin: Oasisbr
Subject(s): Anticorpos Antiprotozo?rios / sangue; Anticorpos Antiprotozo?rios / gen?tica; Anticorpos Antiprotozo?rios / imunologia; Leishmania / imunologia; Leishmaniose Cut?nea / diagn?stico; Prote?nas Recombinantes / gen?tica; Prote?nas Recombinantes / imunologia; Sensibilidade e Especificidade; Testes Sorol?gicos / m?todos; Ensaio de Imunoadsor??o Enzim?tica / m?todos
Description
This work was supported by Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo (grant 2015/22075-8 and fellowship 2016/02622-7 to C.M.S.), by CAPES (fellowship to C.M.S.), by Conselho Nacional de Pesquisa (research fellowship to H.G.), and by Laborat?rios de Investiga??o M?dica (LIM-38-HC, FMUSP). This research was also supported by funding from the Bill and Melinda Gates Foundation under grants OPP49932 and OPP1084251 and by grant R01AI025038 from NIAID, National Institutes of Health.
Universidade de S?o Paulo. Instituto de Medicina Tropical de S?o Paulo. S?o Paulo, SP, Brazil.
Universidade de S?o Paulo. Instituto de Medicina Tropical de S?o Paulo. S?o Paulo, SP, Brazil.
Universidade de S?o Paulo. Instituto de Medicina Tropical de S?o Paulo. S?o Paulo, SP, Brazil.
Infectious Disease Research Institute. Seattle, WA, USA.
Infectious Disease Research Institute. Seattle, WA, USA.
Infectious Disease Research Institute. Seattle, WA, USA.
Oswaldo Cruz Foundation. Aggeu Magalh?es Research Center. Department of Immunology. Recife, PE, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Funda??o de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil.
Funda??o de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil / Universidade do Estado do Amazonas. Manaus, AM, Brazil.
Funda??o de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil
Funda??o de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil
Funda??o de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil / Universidade Nilton Lins. Manaus, AM, Brazil;
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade Federal do Par?. N?cleo de Medicina Tropical. Bel?m, PA, Brazil.
Universidade de S?o Paulo. Instituto de Medicina Tropical de S?o Paulo. S?o Paulo, SP, Brazil / Oswaldo Cruz Foundation. Aggeu Magalh?es Research Center. Department of Immunology. Recife, PE, Brazil.
Universidade de S?o Paulo. Instituto de Medicina Tropical de S?o Paulo. S?o Paulo, SP, Brazil / Universidade de S?o Paulo. Faculdade de Medicina. S?o Paulo, SP, Brazil.
American tegumentary leishmaniasis (ATL) (also known as cutaneous leishmaniasis [CL]) is caused by various species of protozoa of the genus Leishmania. The diagnosis is achieved on a clinical, epidemiological, and pathological basis, supported by positive parasitological exams and demonstration of leishmanin delayedtype hypersensitivity. Serological assays are not routinely used in the diagnosis because many are considered to have low sensitivity and the particular Leishmania species causing the disease can lead to variable performance. In the present study, we generated recombinant versions of two highly conserved Leishmania proteins, Leishmania (Viannia) braziliensis-derived Lb8E and Lb6H, and evaluated both in enzyme-linked immunosorbent assays (ELISA). Recombinant Lb6H (rLb6H) had better performance and reacted with 100.0% of the ATL and 89.4% of the VL samples. These reactions with rLb6H were highly specific (98.5%) when compared against those for samples from healthy control individuals. We then assessed rLb6H against sera from ATL patients infected with different species of Leishmania prevalent in Brazil [Leishmania (Leishmania) amazonensis, L. (Viannia) braziliensis, and L. (V.) guyanensis] and samples from patients with other infectious diseases. In analyses of 500 sera, ELISA using rLb6H detected all 219 ATL samples (sensitivity of 100.0%) with an overall specificity of 93.9% (considering healthy individuals and other infectious diseases patients). Only a minority of samples from Chagas disease patients possessed antibodies against rLb6H, and all of these responses were low (with a highest reactivity index of 2.2). Taken together, our data support further evaluation of rLb6H and the potential for its routine use in the serological diagnosis of ATL.
