Author(s):
David, Susana ; Sutre, Ana Filipa ; Sanca, Armando ; Mané, Alfredo ; Henriques, Victor ; Portugal, Clara ; Sancho, Luísa ; Cardoso, Ana ; Paixão, Eleonora ; Duarte, Elsa Leclerc ; Leite, Clarice Queico Fujimura ; Salem, Julia Ignez ; Antunes, Abílio
Date: 2012
Persistent ID: http://hdl.handle.net/10400.18/1532
Origin: Repositório Científico do Instituto Nacional de Saúde
Subject(s): Mycobacterium Tuberculosis; rpoB; Sputum Smear Microscopy; Sodium Hypochlorite; Denaturing High-performance liquid chromatography (DHPLC) Sequencing; Infecções Respiratórias; Resistência aos Antimicrobianos
Description
The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.
