Author(s): Inácio, Daniel ; Amado, Tiago ; Pamplona, Ana ; Sobral, Daniel ; Cunha, Carolina ; Santos, Rita F. ; Oliveira, Liliana ; Rouquié, Nelly ; Carmo, Alexandre M. ; Lesourne, Renaud ; Gomes, Anita ; Silva-Santos, Bruno
Date: 2025
Persistent ID: http://hdl.handle.net/10400.21/21524
Origin: Repositório Científico do Instituto Politécnico de Lisboa
Subject(s): Cytokines; Gamma delta T cells
γδ T cells producing either interleukin-17A (γδ17 cells) or interferon-γ (γδIFN cells) are generated in the mouse thymus, but the molecular regulators of their peripheral functions are not fully characterized. Here we established an Il17a-GFP:Ifng-YFP double-reporter mouse strain to analyze at unprecedented depth the transcriptomes of pure γδ17 cell versus γδIFN cell populations from peripheral lymph nodes. Within a very high fraction of differentially expressed genes, we identify a panel of 20 new signature genes in steady-state γδ17 cells versus γδIFN cells, which we further validate in models of experimental autoimmune encephalomyelitis and cerebral malaria, respectively. Among the signature genes, we show that the co-receptor CD6 and the signaling protein Themis promote the activation and proliferation of peripheral γδIFN cells in response to T cell antigen receptor stimulation in vitro and to Plasmodium infection in vivo. This resource can help to understand the distinct activities of effector γδ T cell subsets in pathophysiology.
