Author(s): Teixeira, Filipa ; Silva, Ana Margarida ; Sut, Stefania ; Dall'Acqua, Stefano ; Ramos, Oscar L. ; Ribeiro, Alessandra B. ; Ferraz, Ricardo ; Delerue-Matos, Cristina ; Rodrigues, Francisca
Date: 2024
Persistent ID: http://hdl.handle.net/10400.14/45238
Origin: Veritati - Repositório Institucional da Universidade Católica Portuguesa
Subject(s): Intestinal permeation; Lycium barbarum; Phenolic compounds; Response surface methodology; Ultrasound-assisted extraction
Lycium barbarum L. berries have a remarkable chemical composition and extensive biological activities, being a valuable component of health and nutraceutical practices. Nevertheless, a deep insight on the intestinal permeation of the pro-healthy bioactive compounds is urgently needed to predict the real effects on human body. This study attempted, for the first time, to optimize the Ultrasound-Assisted Extraction (UAE) of goji berries using a Response Surface Methodology approach and establish the intestinal permeation of the principal pro-healthy compounds. The optimal extraction conditions were a solid:liquid ratio of 8.75 % for 56.21 min, using an intensity of 59.05 W/m2. The optimal extract displayed a remarkable antioxidant capacity, with LC/DAD-ESI-MS analysis unveiled a diverse phytochemical profile, encompassing different compounds (e.g. glu-lycibarbarspermidine F, 2-glu-kukoamine, rutin, 3,5-dicaffeoylquinic acid). The intestinal co-culture model demonstrated that glu-lycibarbarspermidine F (isomer 2) (73.70 %), 3,5-dicaffeoylquinic acid (52.66 %), and isorhamnetin-3-O-rutinoside (49.31 %) traversed the intestinal cell layer, exerting beneficial health-promoting effects.
