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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
NIH/NIAID
The dynamics of dengue virus (DENV) circulation depends on serotype, genotype and lineage replacement and turnover. In Sao Jose do Rio Preto, Brazil, we observed that the L6 lineage of DENV-1 (genotype V) remained the dominant circulating lineage even after the introduction of the L1 lineage. We investigated viral fitness and immunogenicity of the L1 and L6 lineages and which factors interfered with the dynamics of DENV epidemics. The results showed a more efficient replicative fitness of L1 over L6 in mosquitoes and in human and non-human primate cell lines. Infections by the L6 lineage were associated with reduced antigenicity, weak B and T cell stimulation and weak host immune system interactions, which were associated with higher viremia. Our data, therefore, demonstrate that reduced viral immunogenicity and consequent greater viremia determined the increased epidemiological fitness of DENV-1 L6 lineage in Sao Jose do Rio Preto.
Fac Med Sao Jose do Rio Preto, Lab Pesquisa Virol, Dept Doencas Dermatol Infecciosas & Parasitarias, Sao Jose Do Rio Preto, SP, Brazil
Univ Fed Minas Gerais, Lab Virus, Inst Ciencias Biol, Belo Horizonte, MG, Brazil
Univ Estadual Paulista Julio de Mesquita, Inst Biociencias, Lab Virol, Dept Microbiol & Imunol, Botucatu, SP, Brazil
Fiocruz MS, Ctr Pesquisas Rene Rachou, Lab Entomol Med, Belo Horizonte, MG, Brazil
Univ Estado Amazonas, Programa Posgrad Med Trop, Manaus, AM, Brazil
Fundacao Med Trop Dr Heitor Vieira Dourado FMT HV, Manaus, AM, Brazil
Fiocruz MS, Dept Virol, Ctr Pesquisas Aggeu Magalhaes, Recife, PE, Brazil
Fiocruz MS, Ctr Pesquisas Rene Rachou, Lab Imunol Celular & Mol, Belo Horizonte, MG, Brazil
Univ Fed Rio de Janeiro, Inst Microbiol Prof Paulo de Goes, Dept Virol, Lab Genet & Imunol Infeccoes Virais, Rio De Janeiro, RJ, Brazil
Univ Fed Minas Gerais, Dept Microbiol, Inst Ciencias Biol, Belo Horizonte, MG, Brazil
Univ Fed Minas Gerais, Dept Bioquim & Imunol, Inst Ciencias Biol, Lab Imunofarmacol, Belo Horizonte, MG, Brazil
Univ Texas Med Branch, Ctr Trop Dis, Inst Human Infect & Immun, Dept Pathol,Ctr Biodef & Emerging Infect Dis, Galveston, TX 77555 USA
Univ Fed Juiz de Fora, Lab Virol, Dept Parasitol Microbiol & Imunol, Juiz De Fora, MG, Brazil
Univ Estadual Paulista, Dept Biol, Inst Biociencias Letras & Ciencias Exatas, Sao Jose Do Rio Preto, SP, Brazil
Univ Estadual Paulista Julio de Mesquita, Inst Biociencias, Lab Virol, Dept Microbiol & Imunol, Botucatu, SP, Brazil
Univ Estadual Paulista, Dept Biol, Inst Biociencias Letras & Ciencias Exatas, Sao Jose Do Rio Preto, SP, Brazil
FAPESP: 2012/11733-6
FAPESP: 2013/21719-3
FAPESP: 2015/22750-7
NIH/NIAID: U01 AI115577
