Author(s):
Medina-Alarcón, Kaila P [UNESP] ; Singulani, Junya De L. [UNESP] ; Dutra, Luiz A. ; Pitangui, Nayla De S. [UNESP] ; Pereira-Da-Silva, Marcelo A. ; Dos Santos, Mariana B. [UNESP] ; Ayusso, Gabriela M. [UNESP] ; Regasini, Luis O. [UNESP] ; Soares, Christiane P. [UNESP] ; Chorilli, Marlus [UNESP] ; Mendes-Giannini, Maria J.S. [UNESP] ; Fusco-Almeida, Ana M. [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/200111
Origin: Oasisbr
Subject(s): antifungal; nanostructured lipid system; Paracoccidioides spp.; zebrafish model
Description
Made available in DSpace on 2020-12-12T01:58:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Aim: This study aimed to evaluate the activity of 2′-hydroxychalcone-loaded in nanoemulsion (NLS + 2′chalc), the cytotoxic effect and toxicity against Paracoccidioides brasiliensis and Paracoccidioides lutzii using a zebrafish model. Materials & methods: Preparation and physical-chemical characterization of nanoemulsion (NLS) and NLS + 2′chalc were performed. MIC and minimum fungicide concentration, cytotoxicity and toxicity were also evaluated in the Danio rerio model. Results: NLS + 2′chalc showed fungicidal activity against Paracoccidioides spp. without cytotoxicity in MRC5 and HepG2 lines. It also had high selectivity index values and no toxicity in the zebrafish model based on MIC values. Conclusion: NLS + 2′chalc is a potential new alternative treatment for paracoccidioidomycosis.
Universidade Estadual Paulista (UNESP) Faculdade de Ciências Farmacêuticas Araraquara Proteomics Center Mycology Laboratory
Universidade Estadual Paulista (UNESP) Faculdade de Ciências Farmacêuticas Araraquara Department of Drugs and Medicines
Universidade Estadual Paulista (UNESP) Faculdade de Ciências Farmacêuticas Araraquara Department of Clinical Analysis Cytology Laboratory
Institute of Physics of Sao Carlos IFSC/USP
Paulista Central University Center UNICEP
Universidade Estadual Paulista (UNESP) Instituto de Biociências Letras e Ciências Exatas São José do Rio Preto Department of Chemistry and Environmental Sciences
Structural Genomics Consortium Research Institute-UNICAMP
Universidade Estadual Paulista (UNESP) Faculdade de Ciências Farmacêuticas Araraquara Proteomics Center Mycology Laboratory
Universidade Estadual Paulista (UNESP) Faculdade de Ciências Farmacêuticas Araraquara Department of Drugs and Medicines
Universidade Estadual Paulista (UNESP) Faculdade de Ciências Farmacêuticas Araraquara Department of Clinical Analysis Cytology Laboratory
Universidade Estadual Paulista (UNESP) Instituto de Biociências Letras e Ciências Exatas São José do Rio Preto Department of Chemistry and Environmental Sciences
FAPESP: 2016/05581-0
