Author(s): Cirelli, Thamiris [UNESP] ; Nepomuceno, Rafael [UNESP] ; Rios, Ana Cláudia S. [UNESP] ; Orrico, Silvana R. P. [UNESP] ; Cirelli, Joni A. [UNESP] ; Theodoro, Letícia H. [UNESP] ; Barros, Silvana P. ; Scarel-Caminaga, Raquel M. [UNESP]
Date: 2020
Persistent ID: http://hdl.handle.net/11449/200725
Origin: Oasisbr
Subject(s): interleukin-1beta; interleukin-4; interleukin-6; periodontal diseases; single nucleotide polymorphism
Made available in DSpace on 2020-12-12T02:14:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01
Objective: To assess whether single nucleotide polymorphisms (SNPs) in the IL10, IL1A, IL1B, IL4, TNFA, IL6, OPG, RANK, and RANKL genes, “classically” related with periodontitis, could be associated with susceptibility to T2DM, and also with both diseases concomitantly. Background: There are common pathogenic mechanisms in type 2 diabetes mellitus (T2DM) and periodontitis, but the knowledge of the genetic aspect of this is limited. In patients affected by concomitant T2DM and periodontitis, whose incidence is increasing, there is scarce information regarding the gene-phenotype association, including whether there are genes able to influence both diseases as comorbidities. Methods: Periodontal clinical parameters and biochemical profile (Insulin, Fasting Glycemia, HbA1c, Triglycerides, Total Cholesterol, HDL-cholesterol, and LDL-cholesterol) data were obtained from 894 individuals divided into following three groups: Healthy (H; n = 347), Periodontitis (P; n = 348), and Periodontitis + T2DM (P + T2DM; n = 199). DNA from oral epithelial cells was collected for genotyping. Associations between SNPs and pathologies were tested by multiple logistic regression models, adjusting for age, sex, and smoking habits. We also investigated whether there are sex or smoking effects of each SNP in these phenotypes. Results: The rs1143634-GA (IL1B) SNP showed significantly less likely to develop P + T2DM for all population and mainly for women (adjusted OR = 0.37, 95% CI = 0.16-0.88), while women carrying the rs224320 CT (IL4) were more susceptible to develop P + T2DM (adjusted OR = 1.81, 95% CI = 1.04-3.15). Men carrying the rs1800795-CC (IL6) genotype were less likely to develop T2DM (adjusted OR = 0.12, 95% CI = 0.02-0.70, P =.01). Conclusions: Some SNPs in the IL1B, IL4, and IL6 genes demonstrated sex-influenced association with concomitant periodontitis and T2DM, increasing the evidence of a common genetic component between these diseases and contributing with the understanding of their common pathogenic mechanisms.
Department of Diagnosis and Surgery São Paulo State University—UNESP School of Dentistry at Araraquara
Department of Morphology Genetics Orthodontics and Pediatric Dentistry São Paulo State University—UNESP School of Dentistry at Araraquara
Advanced Research Center in Medicine Union of the Colleges of the Great Lakes (UNILAGO) São José do Rio Preto
Department of Surgery and Integrated Clinic São Paulo State University—UNESP School of Dentistry at Araçatuba
Department of Periodontology University of North Carolina at Chapel Hill—UNC School of Dentistry
Department of Diagnosis and Surgery São Paulo State University—UNESP School of Dentistry at Araraquara
Department of Morphology Genetics Orthodontics and Pediatric Dentistry São Paulo State University—UNESP School of Dentistry at Araraquara
Department of Surgery and Integrated Clinic São Paulo State University—UNESP School of Dentistry at Araçatuba
