Autor(es):
Tairum, Carlos A. [UNESP] ; Santos, Melina Cardoso [UNESP] ; Breyer, Carlos Alexandre [UNESP] ; de Oliveira, Ana Laura Pires [UNESP] ; Cabrera, Vitoria Isabela Montanhero [UNESP] ; Toledo-Silva, Guilherme ; Mori, Gustavo Maruyama [UNESP] ; Toyama, Marcos Hikari [UNESP] ; Netto, Luis Eduardo Soares ; de Oliveira, Marcos Antonio [UNESP]
Data: 2022
Identificador Persistente: http://hdl.handle.net/11449/233190
Origem: Oasisbr
Assunto(s): 2-Cys Prx; Catalytic triad; Chaperone; Hyperoxidation; Oligomerization; Organic hydroperoxides
Descrição
Made available in DSpace on 2022-05-01T05:29:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-07-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Typical 2-Cys peroxiredoxins (2-Cys Prx) are ubiquitous Cys-based peroxidases, which are stable as decamers in the reduced state, and may dissociate into dimers upon disulfide bond formation. A peroxidatic Cys (CP) takes part of a catalytic triad, together with a Thr/Ser and an Arg. Previously, we described that the presence of Ser (instead of Thr) in the active site stabilizes yeast 2-Cys Prx as decamers. Here, we compared the hyperoxidation susceptibilities of yeast 2-Cys Prx. Notably, 2-Cys Prx containing Ser (named here Ser-Prx) were more resistant to hyperoxidation than enzymes containing Thr (Thr-Prx). In silico analysis revealed that Thr-Prx are more frequent in all domains of life, while Ser-Prx are more abundant in bacteria. As yeast 2-Cys Prx, bacterial Ser-Prx are more stable as decamers than Thr-Prx. However, bacterial Ser-Prx were only slightly more resistant to hyperoxidation than Thr-Prx. Furthermore, in all cases, organic hydroperoxide inhibited more the peroxidase activities of 2-Cys Prx than hydrogen peroxide. Moreover, bacterial Ser-Prx displayed increased thermal resistance and chaperone activity, which may be related with its enhanced stability as decamers compared to Thr-Prx. Therefore, the single substitution of Thr by Ser in the catalytic triad results in profound biochemical and structural differences in 2-Cys Prx.
Instituto de Biociências Universidade Estadual Paulista UNESP
Departamento de Genética e Biologia Evolutiva Instituto de Biociências Universidade de São Paulo
Laboratório de Biomarcadores de Contaminação Aquática e Imunoquímica Departamento de Bioquímica Universidade Federal de Santa Catarina
Laboratório de Ecologia Molecular Instituto de Biociências Universidade Estadual Paulista UNESP
Instituto de Biociências Universidade Estadual Paulista UNESP
Laboratório de Ecologia Molecular Instituto de Biociências Universidade Estadual Paulista UNESP
FAPESP: 2007/50930-3
FAPESP: 2011/13500-6
FAPESP: 2013/07937-8
FAPESP: 2017/06263-4
FAPESP: 2018/12628-8
FAPESP: 2019/04054-4
FAPESP: 2020/02868-1
