Author(s):
Silva, Hélder D. ; Beldikova, E. ; Poejo, Joana ; Abrunhosa, Luís ; Serra, Ana Teresa ; Duarte, Catarina M. M. ; Brányik, Tomá ; Cerqueira, Miguel Ângelo Parente Ribeiro ; Pinheiro, Ana Cristina ; Vicente, A. A.
Date: 2019
Persistent ID: https://hdl.handle.net/1822/56379
Origin: RepositóriUM - Universidade do Minho
Project/scholarship:
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F81288%2F2011/PT;
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F72753%2F2010/PT;
info:eu-repo/grantAgreement/FCT/5876/147337/PT;
info:eu-repo/grantAgreement/FCT/5876/147260/PT;
info:eu-repo/grantAgreement/EC/FP7/316265/EU;
Subject(s): Nanoemulsions; Lipolysis; Bioaccessibility; Caco-2cells; Cellular antioxidant activity; Cellular uptake; Science & Technology
Description
Curcumin nanoemulsions stabilized by whey protein isolate were successfully developed using high-pressure homogenization. The effect of a chitosan layer deposition using the layer-by-layer technique on nanoemulsions' stability was evaluated during storage conditions, as well as during gastrointestinal tract passage. Lipids hydrolysis and curcumin bioaccessibility was assessed using a dynamic gastrointestinal model (simulating the stomach, duodenum, jejunum and ileum) and the cytotoxicity, cellular antioxidant activity and permeability analyses were carried out using Caco-2cells. Results showed that both nanosystems were stable during one month of storage and at stomach pH conditions, whereas creaming and phase separation occurred at intestine pH conditions. The addition of a chitosan layer increased curcumin bioaccessibility, whereas cellular antioxidant activity studies revealed that nanoemulsions and multilayer nanoemulsions exhibited 9 and 10 times higher antioxidant capacity at the cellular level, respectively, when compared to free curcumin. Permeability assays showed that the use of a chitosan layer significantly increased the apparent permeability coefficient of curcumin through Caco-2cells by 1.55-folds.
The authors Hélder D. Silva, Ana C. Pinheiro and Miguel A. Cerqueira (SFRH/BD/81288/2011, SFRH/BPD/101181/2014 and SFRH/BPD/72753/2010, respectively) are the recipients of a fellowship from the Foundation for Science and Technology. This work was supported by Portuguese Foundation for Science and Technology under the scope of the Project PTDC/AGR-TEC/5215/2014, of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684), BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte, PEst-OE/EQB/LA0004/2011 and iNOVA4Health - UID/Multi/04462/2013, a program financially supported by FCT/Ministério da Educação e Ciência, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement. The authors also acknowledge the European Commission: BIOCAPS (316265, FP7/REGPOT-2012-2013.1). The support of EU Cost Action FA1001 is gratefully acknowledged. The authors also acknowledge Stepan (The Netherlands) for providing the Neobee 1053 oil, and Rui Fernandes from IBMC, University of Porto for assistance in taking the TEM pictures.
info:eu-repo/semantics/publishedVersion
