Document details

A new model of laryngitis: neuropeptide, cyclooxygenase, and cytokine profile

Author(s): Rodrigues, Manuel Lima ; Fernandes, Ana Valle ; Lamas, Nuno Jorge ; Cruz, Andrea ; Baltazar, Fátima ; Milanezi, Fernanda ; Nunes, Rui ; Reis, R. M. ; Pedrosa, Jorge ; Castro, António G. ; Almeida, Armando

Date: 2008

Persistent ID: http://hdl.handle.net/1822/7897

Origin: RepositóriUM - Universidade do Minho

Project/scholarship: info:eu-repo/grantAgreement/FCT/POCI/46399/PT ;

Subject(s): Laryngeal inflammation; Neuropeptides; Cyclooxygenase-2; Cytokines; Nasogastric intubation; Immunocytochemistry; MRNA expression; Reverse transcriptase polymerase chain reaction analysis; Animal model; Science & Technology


Description

Objectives/Hypothesis: To develop and characterize a new model of laryngeal inflammation by analyzing the presence of neurogenic peptides and expression of cyclooxygenases (COX) and cytokines in the mucosa.Study Design: Laryngitis induced by nasogastric intubation (NGI) was evaluated by histopathologic changes of the mucosa, alterations in calcitonin gene related peptide (CGRP) and substance P (SP) neuropeptides in sensory fibers, and COX-1,2, and cytokine (interleukin [IL]-1, IL-6, IL-10, tumor necrosis factor [TNF]-alpha) expression in the laryngeal mucosa.Methods: Rats submitted to NGI for 1 to 5 weeks were compared with controls. Laryngeal sections were immunostained for stereologic analysis of SP and CGRP fiber density and number of mucosal cells expressing COX-2. Alterations in inflammatory mediators were evaluated by quantitative reverse-transcriptase polymerase chain reaction.Results: NGI induced metaplasia of the epithelium and narrowing of the laryngeal lumen because of hypertrophy of laryngeal glandules and edema. An initial decrease in CGRP- and SP-immunoreactive fibers in the laryngeal mucosa (1-3 wk) was reverted with time (5 wk). COX-2 expression in mucosal cells increased progressively, reaching a maximum level at 5 weeks, and was observed in mononuclear immune cells, which is indicative of a chronic inflammatory process. In regard to mRNA expression levels of inflammatory mediators, TNF-alpha was increased during the 5 week NGI, and IL-10 decreased during the 5 weeks,whereas IL-beta, IL-6, and COX-2 increased in the first 1 to 2 weeks and returned to baseline at 5 weeks.Conclusions: This NGI model results in laryngeal chronic inflammation without direct mechanical aggression of the mucosa and may contribute to the study of future therapeutic approaches to this pathology.

FEDER

Fundação para a Ciência e Tecnologia (FCT) - POCTI/NSE/46399/2002

Fundação Calouste Gulbenkian - projecto nº 74551

Document Type Journal article
Language English
Contributor(s) Universidade do Minho
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