Author(s):
Costa, Lígia ; Carvalho, Alexandre F. ; Carvalho, Ricardo Emanuel Silva ; Rodrigues, Ana Cristina ; Dourado, Fernando ; Deuermeier, Jonas ; Neto, Miguel A. ; Fernandes, António J. S. ; Gama, F. M. ; Costa, Florinda M.
Date: 2024
Persistent ID: https://hdl.handle.net/1822/93690
Origin: RepositóriUM - Universidade do Minho
Project/scholarship:
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT;
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50025%2F2020/PT;
info:eu-repo/grantAgreement/FCT/OE/2020.05233.BD/PT;
Subject(s): Laser-patterning; Bacterial nanocellulose (BNC); Topography; Foreign body reaction (FBR); Immunomodulatory surfaces
Description
The interfacial topography of biomaterials has been identified as a major biophysical regulator of cell behavior and function, a role played through the interplay with biochemical cues. In this work, we demonstrate the potential of laser as a versatile technology for the direct fine-tuning of the topography of Bacterial nanocellulose (BNC) with bioinspired topographies and micropatterns on a cell size scale. Two lasers were used, with different wavelengths---IR (CO2, 10600 nm) and UV (tripled Nd: YVO4, 355 nm) ---attempting to reproduce the Pitcher-plant topography and to create cell-contact guidance patterns, respectively. Different topographies with parallel grooves featuring a 20--300 $\mu$m period were generated on the BNC surface with high fidelity and reliability of the generated microstructures, as demonstrated by 3D optical profilometry and scanning electron microscopy. Moreover, it was demonstrated by X-ray photoelectron spectroscopy that laser processing does not result in detectable chemical modification of BNC. The developed anisotropic microstructures can control cell behavior, particularly regarding morphology, alignment, and spatial distribution. Thus, this proof-of-concept study on the high-resolution laser patterning of BNC opens new perspectives for the development of cell-modulating laser-engineered BNC interfaces, scaffolds, and other advanced medical devices, which can potentially broaden the application of BNC in the biomedical field.
Open access funding provided by FCT|FCCN (b-on). This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic fundings: UIDB/04469/2020 unit, by LABBELS – Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems (LA/P/0029/2020) and i3N - Institute of Nanostructures, Nanomodelling and Nanofabrication (LA/P/0029/2020, LA/P/0037/2020, UIDB/50025/2020). Jonas Deuermeier acknowledges the funding from the contract CEECINST/00102/2018. L. Costa gratefully acknowledges the Portuguese Foundation for Science and Technology (FCT) for the granted scholarship (2020.05233.BD).
info:eu-repo/semantics/publishedVersion
