Autor(es): Singhal, Jaron ; Pinho, Diana ; Lopes, Raquel ; Sousa, Patricia C. ; Garcia, Valdemar ; Schütte, Helmut ; Lima, Rui Alberto Madeira Macedo ; Gassmann, Stefan
Data: 2015
Identificador Persistente: https://hdl.handle.net/1822/51097
Origem: RepositóriUM - Universidade do Minho
Assunto(s): Biomedical applications; Blood cells; Cell-free layer; Cells separation; Micromilling; Microfluidic devices
The most common and used technique to produce microfluidic devices for biomedical applications is the soft-lithography. However, this is a high cost and time-consuming technique. Recently, manufacturers were able to produce milling tools smaller than 100 m and consequently have promoted the ability of the micromilling machines to fabricate microfluidic devices capable of performing cell separation. In this work, we show the ability of a micromilling machine to manufacture microchannels down to 30 m and also the ability of a microfluidic device to perform partial separation of red blood cells from plasma. Flow visualization and measurements were performed by using a high-speed video microscopy system. Advantages and limitations of the micromilling fabrication process are also presented.
