Autor(es):
Barbosa, Thallysson Carvalho ; Nascimento, Lívia Éven Dias ; Bani, Cristiane ; Almeida, Taline ; Nery, Marcelo ; Santos, Rafael Silva ; Menezes, Luana Renyelle de Oliveira ; Zielinska, Aleksandra ; Fernandes, Ana Rita ; Cardoso, Juliana Cordeiro ; Jäguer, Alessandro ; Jäguer, Eliezer ; Sanchez-Lopez, Elena ; Nalone, Luciana ; Souto, Eliana B. ; Severino, Patrícia
Data: 2019
Identificador Persistente: https://hdl.handle.net/1822/61697
Origem: RepositóriUM - Universidade do Minho
Assunto(s): polymeric nanocapsules; poly(e-caprolactone); benzophenone-3; sunscreen; cytotoxicity; ocular tolerance; poly(epsilon-caprolactone); Poly(ε-caprolactone)
Descrição
The objective of this work was to characterize the toxicological profile of a newly developed sunscreen formulation based on polymeric nanocapsules (NCs) loading benzophenone-3 (BZP3). NCs composed of poly(ε-caprolactone) carrot oil and Pluronic® F68 were produced by emulsification-diffusion method. Their mean particle size (Z-Ave) ranged from 280 to 420 nm, polydispersity index (PDI) was below 0.37, while zeta potential (ZP) reached about |+11 mV|. No cytotoxic effects were observed in L929 fibroblast cell line for the blank (i.e., non-loaded) NCs and BZP3-loaded NCs (BZP3-NCs). The semi-solid sunscreen formulation was stable over time (centrifugation testing) and exhibited non-Newtonian pseudoplastic behavior, which is typical of products for topical application onto the skin. The sun protection factor (SPF) value reached 8.84, when incorporating BZP3-NCs (SPF of 8.64) into the semi-solid formulation. A synergistic effect was also observed when combining the formulation ingredients of nanocapsules, i.e., SPF of carrot oil was 6.82, blank NCs was 6.84, and BZP3-loaded NCs was 8.64. From the hen’s egg-chorioallantoic membrane test (HET-CAM) test, the non-irritation profile of the developed formulations could also be confirmed. The obtained results show a promising use of poly(ε-caprolactone) nanocapsules to be loaded with lipophilic sunscreens as benzophenone-3.
