Autor(es):
Esteves, Filipa ; Vilhena Catarino Brito, David ; Rajado, Ana Teresa ; Silva, Nádia ; Apolónio, Joana ; Roberto, Vânia ; Andrade, Raquel ; Calado, Sofia ; Faleiro, Maria Leonor ; Albuquerque Andrade de Matos, Carlos Adriano ; Marques, Nuno ; Marreiros, Ana ; Nzwalo, Hipólito ; Pais, Sandra ; Palmeirim, Isabel ; Simãoa, Sónia ; Joaquim, Natércia ; Miranda, Rui ; Pêgas, António ; Raposo, Daniela Marques ; Sardo, Ana ; Araújo, Inês ; Nóbrega, Clévio ; Castelo-Branco, Pedro ; Bragança, José
Data: 2024
Identificador Persistente: http://hdl.handle.net/10400.1/26482
Origem: Sapientia - Universidade do Algarve
Descrição
Human induced pluripotent stem cells (hiPSCs) hold promises to model and understand human diseases, including those associated with ageing. Here, we describe ABCRIi001-A, a hiPSC line generated from peripheral blood mononuclear cells (PBMCs) of a 79-year-old female enrolled in a study for development of an ageing score (ALFA Score). PBMCs were reprogrammed using three Sendai virus-based reprogramming vectors (hKOS, hc-Myc, and hKlf4). ABCRIi001-A showed normal morphology and karyotype, viral clearance, absence of genomic aberrations, and their pluripotency was confirmed by expression of pluripotency-related markers and their ability to differentiate into the three germ layers. ABCRIi001-A is valuable for ageing-related studies.
