Author(s): Sousa,Lorena R. F. de ; Ramalho,Suelem D. ; Fernandes,João B. ; Silva,Maria Fátima das G. F. da ; Iemma,Mônica R. da C. ; Corrêa,Caroindes J. ; Souza,Dulce H. F. de ; Lima,Maria I. S. ; Vieira,Paulo C.
Date: 2014
Origin: Oasisbr
Subject(s): arginase; Leishmania; galloylquinic acids; Byrsonima coccolobifolia
Leishmanicidal galloylquinic acids were isolated from the ethyl acetate extract of Byrsonima coccolobifolia. These phenols and gallic acid showed to be a new class of potent noncompetitive inhibitors of arginase ARG (Ki ranging from 0.10 to 0.68 µmol L-1) from Leishmania amazonensis. Quinic acid did not exhibit significant inhibition of ARG, indicating that galloyl moiety has important features that allows the enzyme-inhibitor interactions. The significant inhibitory activity of gallic acid on ARG can be a clue to understand the immune response previously observed on L. donovani, since ARG activity is associated with the decrease of the levels of NO in Leishmania infection.
