Author(s): Rodrigues, Ana Carolina Félix ; Fernandes, Natanael ; Diogo, Duarte de Melo ; Ferreira, Paula ; Correia, I.J. ; Moreira, André
Date: 2021
Persistent ID: http://hdl.handle.net/10400.6/12582
Origin: uBibliorum
Subject(s): Photothermal therapy; Polyethylenimine; Hyaluronic acid; Gold-core silica shell nanoparticles; Acridine orange; Cancer
Aims: To develop a tumor-targeted chemo-photothermal nanomedicine through the functionalization of acridine orange (AO)-loaded gold-core mesoporous silica shell (AuMSS) nanorods with polyethylenimine (PEI) and hyaluronic acid (HA). Methods: Functionalization of the AuMSS nanorods was achieved through the chemical linkage of PEI followed by electrostatic adsorption of HA. Results: HA functionalization improved AuMSS' cytocompatibility by decreasing blood hemolysis, and PEI-HA inclusion promoted a controlled and sustained AO release. In vitro assays revealed that HA functionalization increased the internalization of nanoparticles by human negroid cervix epithelioid carcinoma cancer (HeLa) cells, and the combinatorial treatment mediated by AuMSS/PEI/HA_AO nanorods presented an enhanced effect, with >95% of cellular death. Conclusion: AuMSS/PEI/HA_AO formulations can act as tumor-targeted chemo-photothermal nanomedicines for the combinatorial therapy of cervical cancer.
